light reaction rate determines the photosynthetic reaction rate. Experiment Data showed that at 650 nm and a light intensity of 35 m2/sec was a peak. The peak represented the highest points of absorbance. at those points. The absorbance determines was an optimum. Biological concepts of light absorbance support the experimental results. The wavelength at 650 nm is energetically stable and it would be able to efficiently deliver energy for the transfer of electrons. While with higher light intensities
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buffer‚ and chloroplast will be prepared in a number of cuvettes. The cuvettes were tested individually at different wavelengths and intensities to find the optimal rate of photosynthesis by using a spectrophotometer‚ measuring the greatest change in absorbance. From this experiment‚ two data charts and four graphs were obtained. The hypothesis was set from graphs obtained in this lab‚ and the optimal reaction rate was found at a wavelength of 650 nm and an intensity of 50 uEinsteins/m^2/sec. Introduction
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by finding the gradient of a graph of absorbance of the iron complex‚ A‚ against varying values of c‚ concentration of salicylic acid. The value of ε is then taken up to be 1226 M-1cm-1. Calculated results and the graph are as shown below: Table 1: Absorbance against the varying concentrations of Salicylic Acid Standard Solution c‚ [salicylic acid] x 10-4/ M A‚ Absorbance 1 0.733 0.088 2 1.47 0.180 3 2.20 0.277 4 2.93 0.355 Figure 1: Graph of Absorbance of the Iron Complex‚ A = εcl However
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lamberts law and briefly explain why absorbance has no units. In Spectroscopy‚ Beer-Lambert’s Law describes the quantitative relationship between the absorbance of light energy‚ the concentration of the sample solution and the length of the path through the sample. This means that the amount of light at a given wavelength which is absorbed by a solution is proportional to the concentration of the solution. Thus: A= εcl A – measured absorbance ε – wavelength dependent molar
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BIOLOGY DCN PHOTOSYNTHESIS LAB # 6 Effect of varying coloured filters on the photosynthetic rate of spinach chloroplasts The data below is just representative of what trends and relationships you were supposed to see. Numbers can vary. Absorbance at 620 nm for each treatment DCPIP + chloroplasts t=0 min. 0.93 0.945 0.905 0.915 t=3 min. 0.95 0.731 0.83 0.816 change in A620 -0.02 0.214 0.075 0.099 t=0 min. t=3 min. change in A620 t=0 min. t=3 min. change
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L/mol-cm at the wavelength of maximum absorbance intensity‚ λmax = 508 nm. This large value indicates the complex absorbs very strongly. The intensity of the color is independent of pH in the range 2 to 9. The complex is very stable and the color intensity does not change appreciably long periods of time. Beer’s law is obeyed‚ over about 1.5-2 orders of magnitude of iron concentration. Beer’s Law is a very simple relationship: A = εbc where A is the absorbance of a substance at a specified wavelength
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an indicator for many acid-base titrations. When adding different solutions within the indicator it is to react and change colors‚ in this experiment the different colors were blue‚ green‚ and yellow. In the following experiment‚ obtaining the absorbance levels for each one makes it possible to calculate the equilibrium constant. Materials and Methods For this specific experiment there are a few materials that are crucial to finish the experiment. First and foremost a Spectrophotometer will be
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oxoglutarate and NADH in the presence of GLDH to yield glutamate and NAD. Urea + H2O + 2H+ 2NH4+ + CO2 NH4+ + 2-Oxoglutarate +NADH H2O +NAD+ + Glutamate The decrease in absorbance due to the decrease of NADH concentration in unit time is proportional to the urea concentration. Ammonia which is produced by different decomposition processes is also determined by this method. REAGENTS COMPOSITION 5 volume of Reagent 1 contains
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Examination of the Effects of Inhibitory and Non-Inhibitory Competition‚ Enzyme-Substrate Concentration‚ Along with Varying Temperature and pH-Balanced Environments on the Enzyme-Catalyzed Reaction of pNPP Abstract: Introduction: Many of the chemical reactions‚ which take place in in living things are controlled by enzymes. In such cases‚ the enzyme is a protein in the cell which lowers the activation energy of a catalyzed reaction‚ which serves to increase the rate of the reaction. Alkaline
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measured‚ using a graduated cylinder and few of the dissolved Cobalt (II) Chloride Hexahydrate‚ was poured into test tube. The test tube was labeled with a tape‚ to signify 25 mL and the spectrophotometer was used to record the U-V visible ABS (absorbance spectrum) with the wavelength of 515. ● The 75 mL remaining solution was diluted with water‚ filling it to a 100 mL. Also‚ with a graduated cylinder 20 mL of these solution was measured and little was poured into a test tube. The test tube was
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