concentrations containing different volumes of BSA stock for two trials. Sample | Blank | 1 | 2 | 3 | 4 | 5 | Protein( mg/mL ) | 0.0 | 0.2 | 0.4 | 0.6 | 0.8 | 1.0 | Absorbance( 595 nm )Run #1 | 0.000 | 0.570 | 0.796 | 1.140 | 1.580 | 1.760 | Absorbance( 595 nm )Run #2 | 0.000 | 0.488 | 0.710 | 0.985 | 1.240 | 1.580 | Average Absorbance | 0.000 | 0.529 | 0.753 | 1.063 | 1.410 | 1.670 | Figure 1: Trend showing gradual increase of average absorbencies between two trials in
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that the contents are thoroughly mixed. 4. Set the wavelength on the spectrophotometer at the absorption peak for blue solution (620 nm). Using tube No 1. (i.e. ”zero” or “reagent blank”) zero (or standardize) the instrument‚ and then record the absorbance of each solution. 5. Using the spectrometer (wavelength 620 nm) analyse blank‚ standard solutions and unknown concentration CuSO 4 solution. Record absorption of each solution. Note: The unit of wavelength (λ) is nm. 6. You are also provided
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photosynthesis‚ it was possible to observe the reaction as it took place. Samples were created with dark‚ 24 cm‚ 30 cm‚ and 49 cm light treatments. The absorbance was measured in five-minute intervals for each sample and recorded in an appropriate table. When analyzing the data‚ it was observed that the 30 cm and 49 cm samples experienced a dramatic absorbance value decrease after the first five minutes‚ thus showing that the farther the distance from the light‚ the slower the reaction will take place
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The objective of this lab was to recreate the color profile of a given solution. In this case‚ the solution was Powerade. The final solution should match the absorbance values at the peak wavelengths (420nm and 628nm) in Powerade. This lab was done using deionized water‚ FD&C Blue #1‚ FD&C Yellow #5‚ FD&C Red #40‚ and a spectrometer. To obtain the correct color profile‚ FD&C Blue #1 and FD&C Yellow #5 were utilized in the sample solutions. The experiment was conducted over two days; the first day
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cerevisiae (Serrano‚ 1977) We want to know does adding higher concentrations of azide more effectively block dye transport? We tested the transport of dye in yeast cells with a metabolic inhibitor. When we did this we showed no difference in the absorbance between different azide solutions‚ and our control. From this we concluded that azide has no effect on the transport through a yeast cell membrane. Introduction Every cell has a layer of protection called the cell membrane. This cell membrane
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Absorbance of Non-diabetic and Diabetic “Blood Samples” In the experiment‚ I tested the blood glucose level of diabetic and non-diabetic people every 30 minutes starting from right before a meal was eaten up until two hours. I did this by measuring the amount of light absorbed by the solutions through a spectrophotometer. By doing so I found that for diabetics the maximum blood glucose increased more quickly than non-diabetics‚ which increased and decreased at a steady rate. With this information
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banana and reaction rate of this was established with various concentrations of catechol‚ the substrate‚ using the Michaelis-Menten‚ Lineweaver-Burk‚ Hanes-Woolf and Eadie-Hofstee plots. The plots were generated using the slope of absorbance readings against time plots. Absorbance can be used to detect reaction rate as this notes color intensity signaling product formation. The inhibition of o-diphenol oxidase by p-hydroxybenzoic acid is a competitive inhibitor‚ however‚ results in the experiment were
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Lab Report Introduction This lab has the following two concepts: synthesis of acetylsalicylic acid and analysis of acetylsalicylic acid. Synthesis is a purposeful execution of chemical reactions to obtain a product. This concept is used in the first part of the lab; when we have to produce crystals of aspirin. Analysis is the separation‚ identification‚ and quantification of the chemical components of natural and artificial materials. This concept is used throughout the lab when we are analyzing
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enzyme has a region on its surface‚ called the active site‚ which recognizes a specific substrate molecule. The substrate is chemically attached to the active site and binds an enzyme-substrate complex. With the use of a spectrophotometer‚ the absorbance is recorded and the rate of reaction is observed when differing amounts of substrate and
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BIOL2220 Lab 3: Hill Reaction Introduction In 1937‚ Robert Hill discovered that isolated chloroplasts can generate oxygen when they are illuminated in the presence of a suitable electron acceptor‚ even in the absence of carbon dioxide. This finding was a landmark in the study of photosynthesis because it established that the source of the electrons used in the light reactions is water. It also confirmed that the released oxygen is derived from water instead of carbon dioxide. In chloroplasts
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