Record Absorbance Time 2A-Potato extract + cathecol 2B- Potato extract + water 2C- Catechol + water After 10 min 1- What were the substrate‚ enzyme and product of the enzymatic reaction? 2- What is the purpose of tubes 2B and 2C? Enzyme Specificity Table 2 – Specificity of Cathecol oxidase for different substrates Prediction: ____________________________________________________________________________ Absorbance 540nm
Premium Enzyme
clean the cuvette‚ using similar steps earlier. First‚ we rinse with water‚ then with the solution‚ and then we fill it up and test. We tested for the wavelength standard 5 at which there was maximum absorption. At this wavelength‚ we tested the absorbance of all the standards. Table 1: Volumes and Concentrations of Standards and Analysis Standard in ml | | | 0.002M FeCl3 | 0.2M KSCN | 0.1 M HCl | 0 | 5 | 5 | 1 | 5 | 4 | 2 | 5 | 3 | 3 | 5 | 2 | 4 | 5 | 1 | 5 | 5 | 0 |
Premium Chemical reaction Light Concentration
PRACTICAL 7 TITLE : DETERMINATION OF ERYTHROSINE CONCENTRATION USING UV- VISIBLE SPECTROPHOTOMETER INTRODUCTION In chemistry‚ spectrophotometry is the quantitative measurement of the reflection or transmission properties of a material as a function of wavelength. It is more specific than the general term electromagnetic spectroscopy in that spectrophotometry deals with visible light‚ near-ultraviolet‚ and near-infrared‚ but does not cover time-resolved spectroscopic techniques. Spectrophotometry
Premium Spectroscopy Chemistry Light
molecules elute last and then collected in test tubes for examination by spectroscopic techniques. The red/brown proteins‚ in particular‚ will be observed closely as they can either by haemoglobin or cytochrome c‚ using the spectrometer their absorbance readings will determine if they are haemoglobin or cytochrome c. Background research: A technique widely used by scientists for over 30 years gel filtration chromatography purifies biological samples according to their molecular size and
Premium Molecular biology Protein Chromatography
Pre-Lab 1 Assignment Quantitative Analysis of Biomolecules Biochemical analysis involves the characterization of biomolecules within a sample using appropriate laboratory techniques. There are two principal approaches: 1. Qualitative analysis – where a sample is analyzed to determine whether a biomolecule is present or absent. As an example‚ a blood sample might be analyzed for a specific antibody or a bacterial cell might be probed for a nucleic acid sequence. 2. Quantitative analysis
Premium Analytical chemistry Concentration Glucose tolerance test
The Effect of Solvent Exposure and Temperature on Beet Cell Membrane Integrity Introduction: • Cell membranes are vital to proper cellular function • Cell membranes consist of a lipid bilayer made up of phospholipids • Phospholipids are amphipathic molecules meaning they have a dual nature in that they show both hydrophilic (water-loving) and hydrophobic (water-fearing) properties • The amphipathic nature of phospholipids causes them to spontaneously form bilayers in water
Premium Cell membrane Solvent Ethanol
Title Biochemical Analysis of Blood Abstract Introduction After blood is collected from a patient it is analysed to check the fragments present and the fluid which will either be plasma or serum. After plasma is separated from blood it recollects the fibrinogen which in turn helps in clotting however serum is the portion of blood which remains after the fibrinogen is removed. Serum contains approximately 6-8% of the proteins which make up blood however plasma creates up to 55% of blood
Premium Blood Liver Red blood cell
Lab 6 Prelab Spectrophotometry This is due at the beginning of lab 6. Read the lab protocol for this week and type your individual answers to the following questions. Each student should turn in answers at the beginning of class. You may consult other resources (textbook‚ library‚ etc.) Remember to cite information and put responses in your own words to avoid plagiarism (and loss of credit on the assignment). 1. Refer to step 2 in the protocol for lab 6. Calculate the concentration of the sample
Premium Light Chemistry Spectroscopy
calculate the equilibrium constant Kc of formation of FeSCN2+ solution. FeSCN2+(complex ions) solution was able to absorb light due to the properties of complex ions‚ and that property helped us to determine the absorbance of different concentrations of that ions. We saw that absorbance increased as the concentration increased. Finally‚ we found the value of Kc‚ average value of different Kci and were 264.937M-1. The unit depends on the degree of species concentration. 2 Introductions: Chemical
Premium Chemical reaction Chemistry Concentration
spectrophotometric analysis of a penny lab‚ we will be conducting three experiments. The purpose of the first experiment is to find the maximum absorbance on the spectrometer to obtain the optimum wavelength. Once we have gathered that information‚ we will use the results in the other two experiments. The purpose of
Premium Chemistry Chemical reaction Experiment