2007) was utilised to measure the photosynthetic activity in silver beet chloroplasts. The rate of DCPIP reduction was measured at varying distances from the light source. Assays with a spectrophotometer were performed to find and compare the absorbance‚ and hence quantitative effects of light intensity on the chloroplasts. The aim was to determine how the quantity of light affects the rate of photosynthetic activity in silver beet (beta vulgaris) chloroplasts. The greatest rate of photosynthetic
Premium Photosynthesis Light Oxygen
Light‚ Color and Solutions Effects of Concentration and Cell Path Length on a Solutions Absorbance To examine the relationship between color of a solution and the light it absorbs. When it comes down to it our eyes do a accurate job of depicting and distinguishing colors. The purpose of this experiment is to take that a little farther‚ become more precise. With the use of a spectrophotometer one can determine the wavelength and intensity of the light absorbed. By adding a light source to the
Premium Color Chemistry Color wheel
to read. This calibration plot is Abs vs. concentration of ferroin (M) (y-axis vs. x-axis) Use the online resources if you need help figuring out how to plot a graph in Excel. Title the graph and label the axis‚ including the correct units (Absorbance data is unitless). Be sure to double check your units and formatting once you print the report. Add a Trendline to show the linear fit of your data. Choose a linear line andchoose the options that will "display the equation on the chart"‚ including
Premium Analytic geometry
unknown. A spectrophotometer is an apparatus for measuring the intensity of light in a part of the spectrum‚ and is transmitted or emitted on a particular substance. After the number retrieved from the spectrophotometer Beer’s law was used to get the absorbance. The formula was 2-log (% T). This is used in life every day; for example drug companies need this information to see how it affects people. Molarity is moles of solute/liters of solution. In the lab that formula was used when distributing the
Premium Laboratory glassware Concentration Spectroscopy
Enzymes lower the activation energy of chemical reactions but they themselves are not consumed or altered when doing so. These catalysts work best at optimum temperatures and pH’s. The temperature and pH at which the reaction occurs the quickest is the ideal condition for the enzymatic reaction. Alpha amylase converts starch into glucose and when starch is combined with I2KI indicator a dark purple solution forms. As the enzyme breaks down the starch the absorbency will decrease. The absorbency
Premium Enzyme Chemical reaction Catalysis
AN INVESTIGATION OF PHOTOSYNTHETIC ELECTRON TRANSPORT OF CHLOROPLASTS FROM _SILVERBEET LEAVES_ Introduction Photosynthesis is the process by which plants use the energy from sunlight to produce sugar‚ which cellular respiration converts into ATP‚ the "fuel" used by all living things. The conversion of unusable sunlight energy into usable chemical energy is associated with the actions of the green pigment‚ chlorophyll. Light Dependent Reactions are the initial stage of photosynthesis‚ in which solar
Free Photosynthesis Energy
Beer Lambert law‚ the concentration of the solution is directly proportional to the absorbance. The extinction coefficient relates the absorbance to concentration. It is specific for each individual compound and can be determined from the standard calibration curve by solving for ε‚ as I know the absorbance and concentration and can measure the width of the cuvette. Abs = ε bc In this equation‚ the absorbance is equal to the concentration of the CaCl2 (c)‚ multiply by the length of the path
Premium Chemistry Concentration Temperature
place stress on cell membranes‚ detergents make lipids more water-soluble and disrupt membranes‚ and acidic and basic pH’s denature proteins and change the color of the betacyanin. The spectrophotometer will record the absorbance of the betacyanin leakage. The more betacyanin leakage the more the solution will absorb light. Membrane damage increases as the solution gets treated with higher pH and salt concentrations. The experiment supported our
Premium Cell membrane Cell Cell wall
spectrophotometer‚ and then measured the absorbance of Cu(NH3)42+ at different wavelengths‚ in order to determine the optimal one. In our group‚ we determined 600nm was the optimat wavelength with an absorption of 1.32. The chart below shows our absorption rates: We then went on to part two‚ where we used the optimal wavelength to determine a calibration curve for the absorbance of Cu(NH3)42+. We took varying levels of a Cu2+ solution and added it to ammonia an looked at the absorbance amounts‚ which are seen
Premium Chemistry Spectroscopy Ammonia
Group No. 10 Date Started: June 11‚ 2013 Tongco‚ Val Justin DM. Date Finished: June 18‚ 2013 Santos‚ Erickson S. Vasquez‚ Jona Candace P. Wong‚ Victor Lorenzo E. Experiment 1 Use of Micropipettor and Spectrophotometer Introduction In scientific experiments‚ correct measurements are required to achieve precise and accurate data. Precision is the degree to which several measurements provide answers very close to each other. Accuracy describes the nearness of a measurement to the
Premium Accuracy and precision Measurement