= 0.05 The 2nd graph was between concentration and absorbance. This is a direct relationship because as the concentration increased‚ the absorbance also increased. For this graph‚ the line should touch the origin because it is a positive slope going from lower values to higher values. Also it passes through the origin because direct variation relationships are in the form of y = mx‚ where y and m are constant variables. For the absorbance value to zero‚ the concentration must be also be zero.
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production of sugar molecules. However‚ I was only able to determine which of the Tubes experimented had the most or least sugar present‚ by color intensity. By operating a spectrophotometer‚ I was able to create a standard curve‚ which gave the absorbance values of glucose/maltose concentrations in the Tubes tested. From that‚ using the standard curve results and calculations (7.8 x 10^16) I then determined the µmoles of sugar present in each Tube. Finding that‚ in at least 2 minutes‚ enzyme amylase
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Student number: 1701974 Unit code: BHS004-1 Assessment number: 2/5 Word count: You were given five samples of patients’ urine and some Uristix® dipstick. Describe how you used the dipsticks to determine if urine sample contained amounts of protein and / or glucose. To test the given samples for glucose or protein‚ dipsticks were immersed into each of the urine samples for 60 seconds. The colour change was compared with the colour chart on the Uristix® bottle. The detection of glucose in urine
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a cuvette‚ which was placed into the spectrophotometer. The absorbance value was recorded at 590 nm. One hundred twenty five microliters of bromophenol blue and 2875 uL of deionized water (for a solution of 4.167% bromophenol blue) were added to a test tube and were mixed using a vortex. After cleaning the cuvette‚ 1 mL of solution was pipetted out using a p1000 into the cuvette‚ which was placed into the spectrophotometer. The absorbance value was recorded at 590 nm. In the same original test tube
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cyanide. Its reaction is its oxidation to ferricyanide: [Fe (CN)6]4− [Fe(CN)6]3− + e− Spectroscopically the conversion can be followed Now the main Aim of the procedure includes: 1. Determination of absorbance maximum ((max) of potassium ferricyanide in the range 350 nm to 550 nm‚ potassium ferricyanide has the chemical compound with the formula K3[Fe(CN)6] . The salt contains the octahedrally coordinated [Fe(CN)6]3− ion which is bright red in color and
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Enzyme Kinetics and Protein Determination: How Enzyme Catalase Concentration Affects Reaction Rate and Determining the Identity of Unknown Proteins through Absorbance By: Alexander Mak 7238991 Partner: Yasmin Ismail BIO2137 Section A7 Corrector: Chieu Anh Ta September 18‚ 2014 Introduction: The first lab’s primary objective is to observe the different reactions rates amongst the five different catalse concentrations of parsnip. The rate at which the enzyme catalyzes increases in
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photometer which is used in molecular biology. It is used for determining the amount of substance in solution. Basic principle of the spectrophotometer is light transmittance with specific spectrums through the solution and determining the amount of absorbance. As amount of substance in solution increases‚ so more light is absorbed by solution. Spectrophotometer gives quantitative information about amount of substance in solution by determining intensity of not absorbing light. For example‚ to obtain
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spectroscopy is used to determine the components and concentrations of a solution‚ describe various types of spectroscopy‚ describe the visible and ultraviolet regions of the electromagnetic spectrum‚ define Beer’s law and define the relationship between absorbance and transmittance. Other learning objectives are to create a Beer’s law plot for a series of samples with known concentrations‚ collect spectrophotomic data from unknown and known FDC blue dye samples‚ perform serial dilutions‚ calculate concentrations
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Abstract: In this Lab we used the chemical DPIP to detect the rate of succinate broken down by the mitochondrial solution. We detected the amount of DPIP in the solution with a spectrophotometer and measuring the absorbance of light at the 600nm range. DPIP is a useful chemical to use in this experiment because it goes from a blue color when oxidized to a colorless liquid (Ogura‚ 281)‚ this is due to the hydrogen ions and electrons released during the transitional step between succinate and fumarate
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substance to be tested are prepared. * The absorbances of each of the solutions are measured (using the appropriate discharge lamp). * A standard plot is produced of absorbance verses concentration. * When an “unknown” solution is tested the absorbance is noted and applied to the standard graph with the concentration being read directly off the graph. Eg. Plot of absorbance verses concentration: If the unknown solution has an absorbance of x‚ then the concentration will be y: Describe
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