Aim: To know the procedure of measuring the accurate mass of a solid To calculate the number of moles of an unknown mass from its mass and molecular mass To know how to dilute a solution and the effect of dilution on the solution’s absorbance Materials: Copper Sulfate Coloured solution – Potassium Manganate (KMnO4) Hotplate Method: Part A: The Formula of Hydrated Copper (II) Sulfate Firstly‚ about 1.0 g of hydrated copper (II) sulfate was put in the weighing bottle and the colour
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This experiment consisted of two parts. First‚ the absorption of each dye was evaluated by diluting (1 mg/mL standard) 2.0 mL of dye to 100 mL. The absorbance of each dye standard was measured and documented. The absorbance of the Gatorade was then measured by diluting the solution and pipetting 5.0 mL into a 25.0 mL volumetric flask. The absorption spectrum of the chosen drink was measured. Next‚ the purple Gatorade extraction procedure was carried out‚ beginning with pipetting 1 mL of 70% isopropanol
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In conclusion‚ the concentrations and absorbance of the Kool-Aid solution were found. The purpose of the experiment was to develop a deeper understanding of the effect that concentration has on the absorbance of light–which the objective was met. To add‚ another goal of the experiment was to determine the unknown solution’s concentration using the absorbance data recorded. This was concluded to be 0.25 M–most similar to Solution #1. Concentration‚ measured in molarity (M)‚ can be calculated using
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measuring the decrease in absorbance at the wavelength corresponding to the absorption maximum(λmax). The hydrolysis is carried out by using a large excess of NaOH (more than 10-fold) with respect to the substratecrystal violet such that the reaction (CV+ + OH-↔ CVOH) obey the pseudo-first order reaction rate equation -d[CV+]/dt = kw[CV+][OH-]=k[CV+] Where k=kw[OH-] is the pseudo-first order rate constant and kw is the second order rate constant. Assuming the absorbance at the λmax of the reaction
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concentration and absorbance of a solution. A standard curve was experimentally created in this experiment using 10mL solutions of phenol red with concentrations 10µM‚ 7.5 µM‚ 5.0 µM and 2.5 µM then the absorbance of each sample was measured using a spectrophotometer. This generated curve with resulting average absorbances of 1.273nm‚ 1.0275nm‚ 0.585nm‚ 0.285nm and 0.124nm provided a means to determine the phenol red concentrations of two unknown samples after experimentally identifying the absorbance of the
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affect the wavelength for absorbance to occur). Steps to improve experiment: Release the pipette filler slowly to avoid air bubbles. Use a stirring rod to mix the solution. Clean the vials with distilled water. Conclusion Throughout this experiment‚ we found the concentration of ions in sample of unknown solution of nitrite and phosphate. Using the spectrometer we have determined absorbance of various concentrations of nitrite and phosphate solutions. Graphs of absorbance against concentration were
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the highest rate of absorbance while the acid tube had the lowest rate of absorbance. In the following experiment‚ the effects of varying enzyme concentrations on the catalytic rate of ALP were tested. The hypothesis was that enzyme concentration will affect the catalytic rate of ALP. The hypothesis was accepted because the data supports the varying absorbance rates in each of the concentrations. In figure 3‚ cuvette 4a‚ which contained 500 microliters had the highest absorbance rate compared to cuvette
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another 10 mL graduated cylinder and measure out 10 mL of 2.0 x 10-4 c crystal violet solution. 3. Preapre the computer for data collection by opening “Exp 30” from the Chemistry with Computers experiment files of LoggerPro. The vertical axis has absorbance scaled from 0 to 0.35. The horizontal axis has scaled from 0 to 20 minutes. 4. Calibrate the colorimeter for 0% transmittance (no light) and 100% transmittance (maximum light). a) Fill a cuvette ¾ full of distilled water. This is called
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wavelength with maximum absorbance in a green food coloring solution. For this particular solution the wavelength was 629.7 nm. The system was then calibrated to that and was set to measure the food coloring and bleach solution. The measured visible light absorbance of the mixed solution was collected over a time of 200 seconds and eight points were then selected and placed into the Absorbance Spectrum Data Table. The table shows that over time the absorption is decreasing. Absorbance Spectrum Data Table:
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Methods We began this experiment by turning the Spectrophotometer to 605 nm absorbance‚ and setting up each of the tubes as stated in Table 7-2*‚ ignoring the DPIP until the rest of the sample was set up. Once all of the samples were set up accordingly‚ we blanked the spectrophotometer‚ and added the DPIP to the sample right before‚ inverting the sample and taking a reading at the Zero time mark‚ and placing the sample in the designated light apparatus‚ Sample 1 was wrapped in aluminum foil to
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