Read in your lab manual about the following agar mediums: Blood Agar (pg 168)‚ EMB Agar (pg 170)‚ Mannitol Salt Agar (MSA)(pg 172) )‚ MacConkey Agar (pg 174)‚ and PEA Agar (pg 176) to answer the following: 1. What does the blood agar select for? Blood agar allows distinction among bacteria based on their ability to lyse red blood cells (hemolytic activity). 2. What color is the blood agar? Blood red color. 3. What are the 3 types of blood agar results and how can you recognize them?
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“Super clean” and “Magic power”. Principal: Amalyse can catalyse the breakdown of starch into maltose. In this practical‚ solutions of the 2 washing powders will be filled into 2 identical wells on the starch agar plate separately. Starch will be broken down by the amylase disused to the star-agar. A clean zone will be formed around the wells when iodine solution is added and flushed. The higher the amylase activity‚ the more the starch will be broken down. Hence‚ a larger and clearer zone will be observed
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suspension of T4 bacteriophage. Materials: 18 24 hour broth culture of Escherichia coli B. 2 ml suspension of T4 bacteriophages with a titer of at least 10‚000 phages/ml 5 trypticase soy agar (TSA) plates. These should be warmed to 37c before use 5 tubes of soft agar (0.7% agar). Prior to use‚ melt and hold at 50c in a water bath 5 tubes of 9.0 ml trypticase soy (TS) broth 1 ml sterile pipettes Pipette aids Methods: 1. We began the experiment by marking the plates
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The plate count agar‚ CFC agar and cetrimide agar are used and provided different evidences on the isolation of Pseudomonas from the soil experiment. Firstly‚ the plate count agar is a medium for the enumeration of viable organisms in food‚ water‚ waste water and also from clinical samples‚ and thus it is non-selective to any species. Whereas the CFC agar is a selective agar which contains reduced amounts of cetrimide but also cephaloridine and fucidin are added to allow the isolation of most Pseudomonas
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is ironical that his greatness has not been adequately acknowledged or recognized even after 7 years of his death. I would like to begin by quoting from one of Kolatkar’s Marathi poems. main bhAbhiiko bola kya bhAisAbke dyuTipe main a jAu? bhaRak gayi SAli rahmAn bolA goli chalAungA mai bolA ek raNDike wAste? chalao goli.. He translated the poem into English himself as “Three cups of Tea” allow me beautiful I said to my sister in law to step in my brother’s booties you had it coming said
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In each tube a different kind of unknown bacteria was growing on the agar slant. Having the slanted agar allows for more surface area‚ therefore‚ more room for bacteria to grow. For each test there is a general set of rules to follow when transferring bacteria from one culture to another. Disinfect the table surface. Sterilize the transfer
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(in different amounts)‚ which can speed up the breakdown of proteins. As milk-agar plate is a milk protein‚ so when it is incubated with fruit juices containing proteases‚ the milk protein will be broken down and clear zones will appear around the wells containing different fruit juices. Thus‚ the higher the protease activity‚ the larger the diameter of the clear zones. Equation: Control: One well in the milk-agar plate is filled with distilled water to act as a control to show that the formation
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3 antibiotic dicks | Distilled water | Culture of Serratia marcesans | Sterile filter-paper disks | Transparent tape | 1 sterile nutrient agar plate | Metric ruler | Forceps | 1 sterile cotton swab | Safety goggles | Lab apron | | Materials: Procedure: In the Antibiotic Resistant Bacteria lab‚ the effect of different antibiotics on the zone of inhibition on bacteria‚ Serratia marcesans‚ was measured in millimeters. The safety equipment‚ lab apron and goggles were worn at all times
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volume ratio. As the surface area of the Agar cube increases‚ the rate of conductivity will steadily increase‚ too. The trend line of the graph shows as an exponential graph because at one point there will be no more conductivity due to too small or too big Agar cubes. A difference can be seen in the average rate of conductivity depending on surface area because the Agar cubes with a larger total surface area are able to diffuse the ions faster. When the Agar cube has larger surface area‚ more area
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Some food in this world are made using microorganism to produce a desire flavour‚ taste and texture of the food. For examples: yogurt‚ tapai‚ cheese‚ bread and others. Starter cultures is used in these food production. A starter culture is a microbiological culture which actually perform fermentation. These starters usually consist of a cultivation medium‚ such as grains‚ seeds‚ or nutrient liquids that have been well colonized by the microorganisms used for the fermentation. These starters are formed
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