since they are readily carried about by air currents. Septa = hyphal cross walls which divide the filaments into separate cells. Petri Plate (dish) = a special covered dish in which mold is cultured. Medium = a solid nutrient used for culturing. Agar = a non-nutrient thickening agent which is thicker than gelatin but still quite soft. Smear = a thin film of microbial cells on a microscope slide. Fixing = passing the smear through the flame of the laboratory burner three times in rapid succession
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• Eye protection • 250 mL beaker • Timing device • Scoopula • Ruler • Scalpel • Sodium hydroxide solution • 3 different sized cubes of phenolphthalein agar • Paper towels Purpose 1. Put on eye protection 2. With the scalpel cut the block of phenolphthalein agar in to a 1x1x1 cm cube (Cube A)‚ a 2x2x2 cm cube (Cube B) and a 3x3x3 cm cube (Cube C). 3. Pour enough sodium hydroxide solution in to the beaker to cover the
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INTRODUCTION Cassia fistula Linn. (Leguminosae) is a very common plant and is widely known for its medicinal properties. In the Indian literature‚ this plant has been described to be useful against skin diseases‚ liver troubles‚ tuberculous glands and its use in the treatment of rheumatism‚ hematemesis‚ pruritus‚ leucoderma‚ and diabetes.[1‚2] Besides‚ it has been found to exhibit anti-inflammatory and hypoglycemic activity and widely used as a mild laxative suitable for children and pregnant women
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Your Full Name: Lauren Sinay UMUC Biology 102/103 Lab 1: Introduction to Science INSTRUCTIONS: On your own and without assistance‚ complete this Lab 1 Answer Sheet electronically and submit it via the Assignments Folder by the date listed in the Course Schedule (under Syllabus). To conduct your laboratory exercises‚ use the Laboratory Manual located under Course Content. Read the introduction and the directions for each exercise/experiment carefully before completing the exercises/experiments
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the 2 tests: the glass tube setup and the water agar-gel setup. In the glass tube setup‚ two cotton balls were soaked in the solutions of hydrochloric acid (HCl) and ammonium hydroxide (NH4OH) and were simultaneously placed on both ends of the tubing.NH4OH had a lighter molecular weight of 35 g/mole which diffused at a faster rate of 24.8 cm and formed a white smoke near the HCl end that had the molecular weight of 36 g/mole. The water agar-gel setup was made up of a petri dish containing
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the glass tube test and the agar-water gel test. In the glass tube set-up‚ two cotton plugs soaked in two different substances (HCl and NH4OH) were inserted into the two ends of the glass tube. The substance with the lighter molecular weight value (NH4OH‚ M = 35.0459 g/mole) diffused at a faster rate (dAve = 25.8cm)‚ resulting in the formation of a white ring around the glass closer to the side of the heavier substance (HCl‚ M = 36.4611 g/mole; dAve = 10.8 cm). The agar-water gel set up was composed
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for specific organisms. For i.e. Mackonkey agar only grows on gran(-). If a sample is negative it grows and if it is positive it doesn’t grow. Last but not least Differential media relies on color change of the media itself. This color change highlights the bacteria if it has a specific trait. For i.e. If sugar ferments there will be a color change. III. Reagents: 1.Tryptone 2.Yeast extract 3.NaCl 4.KCl 5.MgCl2 6. MgSO4 7.Glucose/ Dextrose 8.H20 9.Agar( for solid) IV. Methods Grab an Erlenhymer
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General Purpose media is designed to grow most organisms and do not contain growth inhibitors. Standard Methods Agar and Blood Agar Bases are examples of general purpose media. Differential Media Differential media contain a component that allow an observable change when a specific chemical reaction takes place. Simmons Citrate Agar is an example of a differential medium. In Simmons Citrate Agar‚ there is a pH indicator that turns from green to blue when citrate is utilized as the sole carbon source
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Lab 11 Methodology By using aseptic‚ a little cultured bacteria was inoculated on the TSA agar. A quadric streak was making. Inoculation loop was heated and keep it cold for a while before the next quadratic streak. Six agar plates were observed for 24 hour at temperature of 30ºC. Choose one from the dense colony and make a sub-culture on the new agar plate. The step was repeated to get a single colony‚ which is pure colony. a) Sequestration of bacteria from fish organs Methodology
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Laguna. B. Maintenance of Microbial Cultures Bacterial isolated were subculture on slants of Nutrient Agar (NA). Incubation of the subcultured specimens for bacteria was done for 7-14 days in Isolation Room. C. Media Preparation for Assay Three tubes with nutrient agar were prepared. The cultures were inoculated onto their respective media by placing 0.2ml of each. The inoculated agar was then poured to individual sterile plates. D. Extraction from Leaves The extraction was done through
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