ANTIMICROBIAL ACTIVITY OF SOIL USED IN THE TREATMENT OF BACTERIAL DIARRHOEABYSITUMBEKO LIWELEYA(s213459531)Research Proposal Submitted in fulfilment of the requirements for the degree of BTECHBIOMEDICAL TECHNOLOGY | | FACULTY OF HEALTH SCIENCES | atNelson Mandela Metropolitan University | MODULE- RESEARCH METHODOLOGYDUE DATE- 16th MAY‚ 2013.SUPERVISOR- PROFESSOR N. SMITH | | Contents ANTIMICROBIAL PROPERTIES OF SOIL USED IN THE TREATMENT OF BACTERIAL DIARRHOEA
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Brain break- what type of medium is blood agar- COMPLEX Differential vs selective vs differential AND selective media Differential- ingredient added that can be changed by certain bacteria in a recognizable way Blood agar Hemolysis Alpha- green Beta- yellow surrounding strep throat Selective Hemolytic Ingredient added that inhibits growth f many unwanted organisms Antibiotic-containing medium Selective and differential MacConkey agar – gram neg that ferment lactose Bile
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utilizing procedures learnt during the semester. Procedures were followed as stated in the lab manual (1). Since the sample contained two unidentified bacteria‚ the first step was to isolate each bacterium using streak plate technique. Tryptic Soy Agar (TSA) plate‚ and differential media such as mannitol salt and Eosin methylene blue (EMB) were used for isolation streak technique. This step is imperative because the bacteria need to be separated and isolated before they can be identified. Moreover
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Frances Schultz West Civilizations II Professor Nardini April 15th‚ 2015 Mein Kampf‚ Adolf Hitler - Response Essay Adolf Hitler was born 1889 in Austria‚ and was a German politician. He was the leader of the Nazi Party; National Socialist German Workers Party (NSDAP). He was chancellor of Germany from 1933 - 1945 and in that time period‚ was also leader of ‘Nazi Germany’. He was an efficient and effective dictator‚ and was at the centre of WWII‚ and of course‚ the Holocaust. Previous to his
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sterilizing it with 70% ethanol. Then trypticase soy agar (TSA) was poured into six groups of 60 Petri dishes (See Appendix 1). The dishes were labeled based on the antibiotic used and were left to dry and solidify at room temperature. After an hour‚ the dishes were placed in a refrigerator at three degrees Celsius. If there was visible condensation on the agar plate‚ they were flipped to avoid the contact of heat that drove the moisture out of the agar dishes. The contents of the broth culture of E.
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CHAPTER I INTRODUCTION Background of the Study Mangrove swamps are forested intertidal ecosystems that occupy sediment-rich sheltered tropical coastal environments. By trapping and stabilizing fine sediments‚ mangroves control the quality of marine coastal waters. Aside from maintaining coastal food webs and populations of animals‚ mangroves have an important role in pollution control through their absorptive capacity for organic pollutants and nutrients‚ and they play an important role in storm
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experiment. In part A of the lab‚ diffusion was demonstrated with two solids and an agar gel petri dish. One crystal of potassium permanganate and one crystal of methylene blue were placed on either side of an agar gel petri dish. The purpose of this experiment was to determine which of the crystals would diffuse across the gel more. So the question is‚ which solid would have a higher rate of diffusion through the agar‚ methylene blue or potassium permanganate? Diffusion is the movement of particles
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antibiotic-producing microbes in the soil that was taken from a local sample. To do so‚ two different samples of bacteria have been taken and placed onto agar plates (labelled A and B). As we allow each sample to grow‚ our objective will be to see what‚ if any‚ antibiotics develop and inhibit the growth of the bacteria. We predict that antibiotics will form on the agar plates‚ thus inhibiting the growth
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of diffusion in agar means it will diffuse faster because it is more concentrated. We graphed the diffusion of chloride ions rather than sodium ions because while the sodium ions do diffuse in to the agar the chloride ions from the NaCl will combine with silver ions‚ from AgNO3 and create a white precipitate. The white precipitate allows us to measure the the white band through the agar at different points and determine the rate of diffusion of the chloride ions. If the the agar concentration was
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Background: In Jane Horack’s article “Staphylococcus epidermidis”‚ S. epidermidis is described as “gram-positive cocci bacteria that are part of the normal flora on the skin and nasal passages.” The article goes on to say that the species was originally named Staphylococcus Albus by microbiologist Rosenback in 1884. When viewed under a microscope S. epidermidis will appear in chains‚ pairs‚ or grape-like clusters (Horak 1). Taxonomically‚ the species S. epidermidis falls in the genus Staphylococcus
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