Purpose: To find how pH and temperature levels affect the enzyme Background: Enzymes are a type of protein vital to sustaining life‚ it works with vitamins and minerals as a biological catalyst‚ which lowers the activation energy for a reaction to occur. Each individual type of enzyme does a specific job‚ and they do not die‚ they are reused. For example‚ catalase is an enzyme found in almost all living cells that will break down hydrogen peroxide and turn it into water and oxygen. The
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Title: Enzyme Activity Aim: To investigate the activity of the enzyme catalase in liver and potatoes‚ and to investigate the effect of temperature‚ surface area‚ pH and certain chemicals on the activity of catalase. Equipment: Dilute hydrochloric acid solution x 1ml 10 volume hydrogen peroxide x 100ml Copper sulphate solution x 1ml Aluminum nitrate solution x 1ml Zinc nitrate solution x 1ml dilute NaOH solution x 1ml Mortar and pestle x 1 25ml Beaker x 1 Hot plate x1
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____________________ Immobilizing an enzyme provides various analytical benefits‚ and can be done in a myriad of ways‚ with the most common being entrapment. For this study peroxidase (from horseradish)‚ an enzyme that catalyzes the cleavage of hydrogen peroxide into water‚ was entrapped within a polyacrylamide gel matrix. The gel matrix was formed by the addition of methylene bis-acrylamide (a cross linking agent) to acrylamide. The immobilized enzyme was then tested via spectrophotometric assay
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FALL 2011 Psychology 100 Final Exam Notes Greta Epstein Chapter 2: RESEARCH METHODOLOGY What is Scientific Inquiry? * A way of finding answers to empirical questions- questions that are answered by observing and measuring * 4 basic goals: * Describing what happens * Predicting when it happens * Controlling what causes it to happen * Explaining why it happens
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LAB 9: DATE: 25TH January‚ 2011. FORM CLASS: L6 3 SUBJECT: Biology TITLE: Enzymes AIM: To investigate the effect of temperature on the enzyme lipase INTRODUCTION: The phenomenon of catalysis makes possible biochemical reactions necessary for all life processes. Catalysis is defined as the acceleration of a chemical reaction by some substance which itself undergoes no permanent chemical change. The catalysts of biochemical reactions are enzymes and are responsible for bringing about almost
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Week 3 Physical Science Lab 2-Doppler Effect XXX-XXXX Course Number: SCI 110 Professor: Karma Pace-McDuffy Due Date: 01/27/2011 Doppler Effect 1 Objectives Measure the detector frequency for waves emitted from a slowly moving source as that source is approaching the detector. (Exploration 1) Calculate the detector frequency for waves emitted from a slowly moving source as that source is moving away from the detector. (Exploration 2) Sketch the wave-front patterns
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SCHOOL OF BIOLOGICAL SCIENCES BS1005 BIOCHEMISTRY 1 Name: Kingston Tan Lee Kang Matriculation Number: U1440619E Tutorial Group: 8 LAB REPORT Practical 2- Macromolecule Structure Study School of Biological Science‚ NTU BS1005 Practical: Macromolecule Structure Study Results & Discussion of Problems Peptide I – PDB I (β – Sheet) Figure 1. ‘Cartoon view’ of Peptide I showing its secondary structure Figure 1 illustrates the secondary structure of PDB file 1 which shows a β – Sheet. 3 hydrogen bond
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are placed into 2 plungers and placed under a lamp the CO2 rate will speed up the process of photosynthesis. Materials: -Fluorescent lamp - Hole punch - 1 Sharpie marker -20 leaf discs -Syringes -12 mL cups Data Table Start 2 4 6 8 10 12 14 16 18 20 High (Bakin g Soda) 1 2 4 4 5 6 6 7 9 9 Low (Water ) 0 0 0 1 2 2 2 3 3 4 Analysis: By increasing the CO2 concentration it will increase the rate of photosynthesis until other factors become limited. 2. The reason a vacuum
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this experiment is to find the width of a piece of hair using diffraction pattern created by a thin film. Hypothesis: If the hair is human it will have a width of 10-4m. Procedure: 1. Place two pieces of glass flat against each other. 2. Obtain a piece of long hair‚ most likely from one group member’s head. 3. Place the hair between the sheets of glass on one edge. 4. Place a rubber band around the pieces of glass on the side opposite the hair for stability. 5. Measure the
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TDDC17 -‐ Lab 3 Part 2 Q5 P (Meltdown) = 0‚02578 P(Meltdown | Ica weather) = 0.03472 b) Suppose that both warning sensors indicate failure. What is the risk of a meltdown in that case? Compare this result with the risk of a melt-‐down when there is an actual pump failure and water leak. What is the
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