EXPERIMENT 2 ACID DISSOCIATION CONSTANT OF AN INDICATOR DYE OBJECTIVES Using spectrophotometric method: determine the wavelengths at which the acid and base forms of the dye in aqueous medium exhibit maximum absorption; determine the molar absorptivities of the acid and base forms of the dye and estimate an unknown concentration of the dye in solution using the Beer-Lambert’s Law; and determine the acid dissociation constant of the indicator dye. THEORY The absorption or reflection of
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expected melting point. After its temperature reached the plateau‚ the temperature increment was set at 1°C per minute‚ and the melting point range of 152°C-154°C was recorded. After standardizing the melting device‚ a small sample of unknown carboxylic acid was placed in a capillary tube‚ and an arbitrary plateau of 200°C was set. When the sample started to melt at 150°C‚ another capillary tube containing a small amount of unknown sample was placed in the melting device‚ and the plateau was set to 135°C
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This paper is an analysis conducted on Nucleic Acids through a variety of tests specifically‚ Dische‚ Murexide‚ Wheeler-Johnson and Phosphate Tests in order to exemplify structural features of nucleic acids as well as identify the principle involved in each chemical test. Different procedures and different test compounds were applied‚ and results were noted as for changes in colors of precipitates or solutions. For Dische Test‚ light blue was obtained for RNA and dark violet for DNA. For Murexide
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Affects of Hydrogen Peroxide Equivalents on Green Synthesis of Adipic Acid. Andrew R. Glessman*‚ Chase W. Turner‚ Audra Cokain‚ Jacob Kindred‚ Darryl Watkins Department of Chemistry and Chemical Biology‚ IUPU‚ 402 N. Blackford St‚ Indianapolis‚ IN 46202 aglessma@umail.iu.edu April 25‚ 2014 Figure _______________________________________________________________Abstract- The synthesis of adipic acid has been a growing topic of discussion due to the harmful impact it has on the environment
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Extraction of Caffeine from Tea Leaves Marc Tugaoen‚ Kristine Vanzuela‚ Rafael Villanueva‚ Justeen Wong Department of Chemistry‚ University of Santo Tomas‚ Manila‚ Philippines Abstract This experiment has been divided into 4 set-ups‚ first was the solid-liquid extraction‚ next was the liquid-liquid extraction‚ then the sublimation and last was the melting point determination. The solid-liquid and liquid-liquid extraction were both done during the first meeting‚ the DCM layer was filtered and
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EXTRACTION OF COPPER FROM OXIDISED COPPER ORE Aim: To analyse how copper is obtained from oxidised copper ore using the processes of leaching‚ solid-liquid separation‚ displacing and electrowinning. Method: Refer to ‘Extraction of Copper from oxidised Copper Ore’ document pages 1-2 Results: Leaching: The blue particles in the copper ore which is copper was dissolved in the sulphuric acid and the solution turned into a blue colour. There was also sediment that was floating around
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Jarrett Sears Dr. Hummel Chem 121 2/7/13 A Chemical Reaction between Tin and Nitric Acid Introduction: Finding the products made from the components of tin metal and nitric acid is the ultimate objective of this lab; the formula of the products will be found in the CRC handbook thereafter. Procedure: First‚ obtain a clean crucible‚ and heat it under a Bunsen burner until it is as hot as possible‚ about 15 minutes within a fume hood unit. The Bunsen burner will be used under the fume
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Punished by Acid Several people from the third world become a victim of an acid attack. The victim’s life has changed tremendously‚ which is what the photographer shows with his world-known pictures. On Ebrahim Noroozi’s photoseries you see a mom named Somayeh and her daughter‚ Rana and what acid has done to their faces. Now they most stay by themselves and take care of themselves. It is hard for me to understand some people will walk up to you to pour acid on you. Somayeh looked like an average
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Properties of Acids and Bases Diagram: A B C D E F Bromothymol Blue Universal Indicator Phenolphthalein Litmus Paper Analyze: 1) F‚ C‚ D‚ B‚ A‚ E 2) D is neutral because it turned to the colour of green when it came in contact with the indicator Bromothymol Blue. The acid or base is green between 6.0-7.6 pH. 3) Solution E is more alkaline (more basic) than solution A because when E came into contact with the indicator Bromothymol Blue it turned
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The purpose of this experiment was to study the effect acid rain has on seed germination and after performing this experiment is can be concluded that acid rain has a negative effect on seed germination. When seeds are soaked in an acidic solution the process of germination slows. Acidic solutions‚ while do slow growth of a seed‚ they do not kill the seed. A seed can be dormant for a majority of its lifespan‚ seeds watered with acid solutions stayed in a dormant condition. For example‚ trial three
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