787122 Received 3 October 2002/Accepted 21 November 2002 Downloaded from http://aem.asm.org/ on June 6‚ 2013 by UNIVERSITY OF DELHI Despite their commercial importance‚ there are relatively few facile methods for genomic manipulation of the lactic acid bacteria. Here‚ the lactococcal group II intron‚ Ll.ltrB‚ was targeted to
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Acid-Base Titrations A Titration of Potassium Hydroxide Phthalate and Sodium Hydroxide Hanna Piper Department of Chemistry‚ SUNY College at Brockport‚ Brockport‚ NY 14420 Chemistry 205.06 Abstract Titrations are used to find the molarity of an unknown solution. A titration begins with an analyte and titrant being used to measure the unknown molarity of the analyte. In the following experiment‚ sodium hydroxide was used as the titrant and potassium hydroxide phthalate was used as the
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As the assistant to Michael Brune‚ the executive director of the Sierra Club‚ I am in full support of The Exploratory Fracking and Extraction act. I would like to express concerns associated with hydraulic fracturing‚ or fracking‚ on behalf of the Sierra Club. Critical problems that are commonly brought to attention are: contamination of water supplies‚ seismic activity caused by fracking‚ and it worsens the current condition of the climate. Fracking wells inject water‚ sand‚ and chemicals deep
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Objective: The objective of this experiment is to use acid-base extraction techniques to separate a mixture of organic compounds based on acidity and/or basicity. After the three compounds are separated we will recover them into their salt forms and then purify them by recrystallization and identify them by their melting points. Procedure: Extraction of Carboxylic Acid A pre-weighed (0.315g) mixture of Carboxylic acid‚ a phenol‚ and neutral substance was placed into a reaction tube (tube 1)
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microfuge tube. Place this tube directly into liquid N2 to freeze plant tissue. 2) Remove tubes from the liquid N2 and quickly grind the plant tissue with a pestle. Add 300 L of Methanol 1%HCl and continue to grind the plant tissue. Allow extraction to occur overnight in a dark refrigerator. 3) Add 200 L Milli-Q H20 to each tube. Next‚ go to the fume hood and add 500 L of chloroform to each tube. Spin the tubes in a centrifuge set at the highest rpm for 2-5 minutes. 4) Return to
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Acids and Bases Shane Purpose- To study the relationship between pH and metabolism. 10/21/13 Materials- see attached Per. D Biology Procedure- see attached Data and Results- see attached Theory- PH stands for the power of hydrogen. People refer to the pH scale as a tool for finding out if a substance is acidic‚ basic‚ or neutral. This scale goes from zero to fourteen. The number seven is neutral. Any number higher than seven on this scale is basic. Any number lower is acidic
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There has been much debate in recent years among politicians‚ military officials‚ and civilians on whether or not the U.S. should be fully withdrawn from Afghanistan‚ regardless of the country’s economic and military capabilities. There has also been a proposed timetable‚ which President Obama says is in effect and currently being executed‚ to have up to ninety percent of our military personnel out of the country by no later than 2014. It is imperative that we extract our forces out of Afghanistan
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Extraction of Caffeine from Tealeaves Perez‚ Albert Solomon K.*; Quiambao‚ Marie Angela C;.Pascua‚ Hanna Harriette R.; Patricio‚ Maria Roxanne DC.; 2-Chemistry‚ Department of Chemistry‚ College of Science University of Santo Tomas España St.‚ 1015‚ Manila Abstract Caffeine is soluble in both water and organic solvents‚ solid-liquid extraction and liquid-liquid extraction were used in the experimentation process. Caffeine was extracted using hot water‚ however‚ due to its
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Laboratory #16: Analysis of Grease in Water by Solvent Extraction Experiment Date: 2/19/10 Due Date: 2/26/10 II. Objective The goal of this lab was to determine the amount of impurity‚ in the form of oil‚ in water using the method of solvent extraction‚ also known as liquid-liquid extraction‚ a method of separating two materials with different physical and/or chemical properties. Two methods of evaporation will be used to finally determine the concentration of impurity- one using an explosion-proof
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Extraction of Caffeine from Lipton Yellow Label Teal Leaves Arlie Bamiano‚ Jealine Bernabe‚ Petrenne Caimbon*‚ Jhia Caso Department of Biology‚ University of Santo Tomas Abstract In order to extract pure caffeine from Lipton Yellow Label tea leaves‚ several extractions and phase transition techniques were employed to 6.5029 grams of sample. Initially‚ the tea leaves were boiled in water to extract tea from the leaves (Solid-Liquid Extraction). After extracting the tea‚ several steps of
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