34. Mixture for overlapping PCR: 1 μL of Herculase II Fusion DNA polymerase‚ 10 μL of 5X Herculase II reaction buffer‚ 0.5 μL of a 100mM solution of dNTPs (25 mM each)‚ 2 μL of a 10 μM solution of each primer‚ 100 ng of each upstream and downstream fragments‚ 200 ng of pyrG marker fragment and adjust to 50 μL of double-distilled water. 35. Lysis Buffer: to prepare 50 mL of buffer dissolve 23.6 g of Guanidine thiocyanate (118.16 g/L) in 25 mL of double-distilled water. Once dissolved add: 2.5 mL of
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Publication No. 11137 Enzyme Activity Guided Inquiry Lab Turnip Peroxidase Introduction Peroxidase enzymes are widely distributed in plants and animals‚ including bacteria‚ to protect cells against the effects of oxidative stress and cell damage due to hydrogen peroxide. Peroxidases are easily extracted from turnips and other root vegetables and provide a model enzyme for studying enzyme activity—how the rate of an enzyme-catalyzed reaction depends on biotic and abiotic factors. Enzyme activity
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Assess the likely success of a buffer stock scheme for natural rubber (25 marks) A buffer stock scheme is when the government seek to stabilise the market price of agricultural products e.g. purchasing supplies when the harvests are plentiful and selling stock when supplies are low. As stated in the study‚ the price of natural rubber has increased as a result of “responding to a combination of demand and supply factors”. The introduction of this scheme can help smoothen the fluctuation in prices
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SUPPLEMENTARY MATERIAL I Unit V: States of Matter 5.7 KINETIC ENERGY AND MOLECULAR SPEEDS Molecules of gases remain in continuous motion. While moving they collide with each other and with the walls of the container. This results in change of their speed and redistribution of energy. So the speed and energy of all the molecules of the gas at any instant are not the same. Thus‚ we can obtain only average value of speed of molecules. If there are n number of molecules in a sample and their individual
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through. The shorter molecules migrate faster than the longer molecules. The use of electrophoresis buffer in the making of the agarose gel is to establish a constant pH and to provide ions to support the conductivity. There are three common buffers used in agarose gel electrophoresis. They are Tris-Acetate-EDTA (TAE)‚ Tris-Borate-EDTA (TBE) and Tris-phosphate. For this experiment‚ the best choice of buffer to use is TAE. The result of our experiment showed no bands. The reasons can be whether the concentration
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int biosdisk(int cmd‚ int drive‚ int head‚ int track‚ int sector‚ int nsects‚ void *buffer); Description biosdisk accesses interrupt 0x13 for BIOS disk operations on the drive specified by the drive argument. For floppy drives‚ 0 identifies the first drive‚ 1 is for the second drive‚ and so forth. This function is not available under DOSX. cmd specifies the disk operation to perform. head‚ track‚ sector‚ nsects‚ and buffer provide further information for the command specified bycmd. The following
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Liquids & Solids Pre Lab Questions: 3pts 1. What are the learning goals of this lab? The learning goal of this experiment is to view and experience the melting point and boiling point of different substances. 2. Why is acetamide not allowed in contact with your skin? What precautions does one have to take to work with this chemical? Acetamide is a carcinogen‚ which can cause cancer. Therefore‚ it should not be allowed to come in contact with a person’s skin. Any person working with this chemical
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percentage (%) solutions and molar (M) solutions. We also have to understand osmotic concepts. Last but not least‚ we have to know about acid-base balance. pH‚ acid and bases‚ and buffer system. Acid is substance that dissociates into hydrogen ions (H+). Base is a substance that dissociates into hydroxyl ions (H-). Buffer is a solutions containing substance that have the ability to minimize change in the pH when an acid or base was added into it. Acid and base balance in human body is essential for
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PURPOSE The purpose of this experiment is to become familiar with the separation of mixtures of solid and learn separation techniques based on the chemical properties of a substance. PROCEDURES 1. Separating out the Iron a. Use your digital scale to determine the mass of your weighing dish. b. Empty the entire mixture of solids from the plastic bag into the weighing dish and determine the gross mass of the total mixture and weighing dish. Compute the net mass of the mixture: this is equal
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Background Information Part 1 In the first part of the enzyme lab‚ we mixed a substrate and an indicator with an enzyme. There was also a neutral buffer in each of the chemical mixtures. The neutral buffer regulated the pH to around 7. We got a color palette and once we mixed each together‚ we observed and saw a change in the color of the substance. The darker and more brown the substance got‚ the more oxygen produced by the reaction. Our results showed that amount of oxygen produced increased
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