Task 3: How could Thin Layer Chromatography (TLC) have been used to prepare a pure sample of aspirin? The method of using thin layer chromatography is easy‚ quick and relatively cheap to carry out this makes it ideal for preparing pure samples of aspirin. Equipment Required: • Thin Layer Chromatography Paper • Ruler • Pencil • Solvent • Beaker • Micro Capillary • Access to a fume cupboard • UV light • Clingfilm • Individuals sample of aspirin • Pure sample of aspirin • Sample of salicylic acid Method:
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Lab #3: Ion Exchange Chromatography Objective The purpose of this experiment was to separate proteins on the basis of their net charge at a particular pH. In cation exchange chromatography positively charged molecules are attracted to a negatively charged column. Conversely‚ in anion exchange chromatography‚ negatively charged molecules are attracted to a positively charged column. Experimental results could be monitored in a predictable way by controlling running pH‚ salt concentration‚ and by
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wavelengths of light for pigments present in Coleus plants Joseph Yung (King Yung) 212831426 Adrian Ionescu Section M 11 February 5‚ 2014 Absorbance Table Absorbance Spectra Figure 1: Absorption spectra of pigments found‚ through chromatography‚ within Coleus plants. The different wavelengths of light were determined by the use of a spectrophotometer Questions 1. The “total pigment” absorption spectrum
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Solubility as a Property of Matter A Lab of Chemicals‚ Chromatography‚ and Crime! Chemistry is a natural science that deals with the composition of matter and the changes it undergoes. At crime scenes‚ investigators often find unknown materials that need to be identified. If an unknown material is a mixture‚ an investigator may want to know one or two things about it: What are the ingredients of the mixture? Is the mixture found at the scene the same as a known mixture? A mixture is a collection
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in the BIOL 1F90 Laboratory Manual (Martin‚ 2013)‚ Experiment # 1‚ pages 2-5. Results: Table 1: Culture Sample Chromatography Rf Values Sample of Spot Distance from Origin (mm) Solvent Front (mm) Rf Value Color A 6.0 57.0 0.22 Bluish green B 17.5 56.0 0.105 Dark green blue C 4.0 57.0 0.070 Greenish blue D 4.0 47.0 0.085 Green E 10.5 56.5 0.186 Light blue Figure 1: Chromatography- TLC sheet From left to right- acetic acid‚ citric acid‚
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FEASIBILITY OF PRUSSIAN BLUE POWDER (TINA) AS PIGMENT FOR WHITEBOARD INK An Investigatory Project Presented to the Science Department Paliparan II National High School – Congressional Annex Brgy. Paliparan II‚ Dasmarinas City‚ Cavite By: Hidalgo‚ Briggs Lansang‚ Marinella Ranoco‚ Jessa Lonosa‚ Jamaicah Lumapas‚ Christopher Lawrence March 2014 Acknowledgement Abstract TABLE OF CONTENTS LIST OF APPENDICES Appendix 1. Sample Research Instrument Appendix
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1 TOPIC 4 Course Learning Outcomes Able to : 1. Explain the fundamental concepts & theories of separation techniques in SFC & SFE. 2. Sketch‚ label the schematic diagrams & discuss the function of each component in SFC & SFE. 3. Identify the strength & limitations of SFC & SFE technique. 4. Suggest and justify the most suitable & efficient separation technique to be employed for an analysis. 2 What is supercritical fluid? 3 Critical temperature (Tc) for any substance is a temperature
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5.05 Mixtures and solutions Lab report Ink Chromatography Chromatography is also used to compare and describe chemical substances. The chromatographic sequence of sorbed substances is related to their atomic and molecular structures. A change in a chemical substance produced by a chemical or biological reaction often alters the solubility and migration rate. With this knowledge‚ alterations or changes can be detected in the substance. Chromatography serves mainly as a tool for the examination and
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In order to purify CelB2 protein bound to maltose-binding-protein‚ amylose affinity chromatography was performed. The amylose resin present in 20% ethanol was first diluted by adding 10 mL of 20 mM TrisHCl‚ pH 7.4‚ 0.2 M NaCl‚ 1 mM EDTA and centrifuged at 700 rpm for 5 minutes. After decanting the buffer‚ another 10 mL of TrisHCl‚ pH 7.4‚ 0.2 M NaCl‚ 1 mM EDTA was added to this resin solution and centrifuged at 700 rpm for another 5 minutes to further dilute the ethanol concentration in the resin
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Gas chromatography is the common method used for the biogasoline analysis. Biogasoline catalytic cracking process requires detailed qualitative and quantitative analysis of the hydrocarbon composition of its oil and fraction. In this analysis‚ their fractional composition‚ saturated vapor pressure‚ density and antiknock rating will be investigated. By knowing the qualitative and quantitative composition of the gasolines‚ the content of n-paraffin‚ isoparaffin‚ aromatic‚ naphthene‚ and olefin (PIANO)
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