LAB EXERCISE: Diffusion and Osmosis Laboratory Objectives After completing this lab topic‚ you should be able to: 1. Describe the mechanism of diffusion at the molecular level. 2. List several factors that influence the rate of diffusion. 3. Explain why diffusion is important to cells. 4. Describe a selectively permeable membrane‚ and explain its role in osmosis. 5. Define hypotonic‚ hypertonic‚ and isotonic in terms of relative concentrations of osmotically active substances. 6. Discuss
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Colligative Properties & Osmotic Pressure (Lab – Chapter 13) Introduction: In this lab‚ we are going to observe the difference in freezing point between pure water and salt water. We will also observe the permeability of an egg shell membrane and dialysis tubing‚ acting in place of a human cell‚ when placed into a hypertonic or hypotonic solution. We will observe the changes in size‚ shape‚ and characteristic. Materials and Methods: First we begin this experiment by making an ice bath in the 100mL
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cells. In experiment 5.3 we test the osmolarity of plant cells through the usage of potato tuber cells. In experiment 5.1 in order to observe diffusion we examine the Brownian movement by observing carmine on a wet mount slide‚ and by using dialysis tubing‚ we investigate the selective permeability to reducing sugar‚ glucose‚ starch‚ and iodine potassium iodine. In experiment 5.2 we use samples of ox blood and Elodea plant to observe the net flow of water from the surrounding solution into the cells
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with 3 atoms‚ then glucose with 24 atoms‚ than sucrose with about 48 atoms‚ and then finally starch. Materials: Refer to lab book Procedure:ia Refer to lab book Data: Refer to lab book Analysis: The purpose of the dialysis bag containing only water in a cup of water was to display an isotonic system. It was also to display osmosis in the water and it was used as a selectively permeable membrane to model the cell membrane. . This first system could be considered the
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1A: Diffusion The materials include a 30-cm piece of 2.5-cm dialysis tubing‚ 15-mL of the 15% glucose/1% starch solution‚ 250-mL beaker‚ distilled water‚ 4-mL Lugol’s solution‚ and string. EXERCISE 1B: Osmosis The materials used include 25-mL of these solutions: distilled water‚ 0.2M sucrose‚ 0.4M sucrose‚ 0.6M sucrose‚ 0.8M sucrose‚ and 1.0M sucrose‚ scissors‚ string‚ a balance‚ six 250-mL cups‚ and six 30cm strips of dialysis tubing. EXERCISE 1C: Water Potential The materials that were
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Material and Methods * Osmosis : -thistle funnel tube -dialysis tubing sack -saturated NaCl w/food coloring -beaker with distilled H2O The experiments begins with the construction of a simple osmometer by obtaining pre-soaked length of tubing ‚opened and tied at the end. The tube is filled with saturated NaCl solution (with added food coloring) and the thistle is inserted in the dialysis tubing. The dialysis tubing is sealed to the thistle funnel with dental floss and placed in a beaker
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Measure and pour 50 mL of water into a 100 mL beaker. Cut a piece of dialysis tubing 15.0 cm long. Submerge the dialysis tubing in the water for at least 10 minutes. I measured and poured 82 mL water into a second 100 mL beaker. While the dialysis bag was soaking‚ made the glucose/sucrose mixture. Using a graduated pipette to add five mL of glucose solution to a third beaker and labeled it “Dialysis bag solution”. Used a different graduated pipette to add five mL of starch solution
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free water across a membrane. The purpose of the study was to calculate the rate of osmosis in artificial cells containing different concentrations of sucrose and water. We studied the rate of osmosis in artificial cells by creating five different dialysis bags with different concentrations of both sucrose and water and calculating the cumulative change in weight ever 10 minutes for 90 minutes. Our results for the artificial cells showed different concentrations moved from high to low concentrations-
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selectively permeable membrane moves from an area of higher concentration to an area of lower concentration. In this lab‚ we can determine the direction of diffusion of small molecules by measuring the diffusion of small molecules through dialysis tubing. This tubing acts as a selectively permeable membrane‚ and allow to pass larger molecules slowly. Introduction: Cells have kinetic energy and it causes the molecules of the cell to move around and bump into each other. Diffusion is one result of
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concentrations of sucrose on the mass of a potato cell. Results for Part One suggested that the molecular weight of albumin and starch was too large to pass through the dialysis tube‚ but glucose and sodium sulfate molecules were small enough to pass through the dialysis tube. Also‚ a decrease in water weight occurred due the dialysis tube being placed in a hypertonic solution. Results for Par Two showed the potato cell having a molar concentration of 0.2734‚ which caused sucrose concentrations above
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