For this experiment‚ we started off by taking tubes numbered 1-4 and started adding one scoop of our enzyme catalyst‚ in this case‚ the yeast. We then proceeded to measure and add 1 mL of distilled water to test tubes A-D. To get a more accurate measure of 1 mL of distilled water‚ we used the dropper labeled “W” to drop distilled water into the 5 mL graduated cylinder until we saw that the bottom of the water line reached closely to 1 mL. Next‚ we took the four tubes with the scoop of yeast and
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Objective: The purpose of this lab is to allow you to become familiar with a procedure for extracting DNA‚ collecting DNA samples‚ and to observe the physical characteristics of DNA Background: The wild strawberry is a diploid because it only has two sets of chromosomes. While the grocery store kind is an octoploidy because it has eight sets of chromosomes. The reason we chose the grocery store strawberry is because we’d be able to extract more DNA. Ripe strawberries make enzymes which help break down the
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EXTRACTING AND PURIFYING GENOMIC DNA FROM A RAT LIVER FOR ELECTROPHORESIS Deoxyribonucleic acid (DNA) is a molecule that encodes the genetic instructions used in the development and functioning of all known living organisms and many viruses. Along with RNA and proteins‚ DNA is one of the three major macromolecules essential for all known forms of life. Genomic DNA is the DNA that holds the complete set of genetic data for an organism. In humans‚ the genomic DNA spans 46 chromosomes‚ providing a complete
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Lab Report (Effect of concentration on enzyme activity) Biology Noor Alawadhi 11- KC Introduction: An Enzyme is a protein‚ which is capable of starting a chemical reaction‚ which involves the formation or breakage of chemical bonds. A substrate is the surface or material on or from which an organism lives‚ grows‚ or obtains its nourishment. In this case it is hydrogen peroxide. This lab report will be explaining the experiment held to understand the effects of the changes in the amount of
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Roy Levin Bio 11 Lab Dr.Izquierdo Analysis of Macromolecules in Tissue Homogenates of Bos taurusMaterials and Methods The homogenates provided were made by homogenizing tissues in a sucrose phosphate buffer in a 1:20 ratio. The protein concentration in bovine cells was measured by diluting the homogenate with a 1:5 ratio; 50 microliters of homogenate and 200 microliters of water. Then 5 known protein concentration samples which were 0.4‚ 0.8‚ 1.2‚ 1.6‚ 2.0 mg/ml of bovine serum were used to
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Cavanaugh Lab: Tuesdays at 1pm Enzyme Kinetics Lab Introduction: Enzymes are proteins that will catalyze reactions to make the rate of the reaction occur faster than it would without. It can also make the reaction occur in the first place. Tyrosinase is an enzyme that has a variety of functions and activities. It produces pigments like melanin and others that would be apparent when a fruit is cut in half and it browns. (Bien-etre 3).There is that one function that stands out and the enzyme is continuously
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this‚ forensic scientists are forced to "bring out the big guns." Researchers can compare DNA samples from bodies to those taken directly from the victim: from hair‚ a toothbrush‚ a family member‚ and etcetera (Whitfield 6). As a result of the terrorist attacks‚ forensic scientists have come up with innovative tests and ways to interpret the resulting identification data (Whitfield 1). According to forensic DNA expert John Butler (National Institute of Standards and Technology in the city of Gathersburg
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The purpose of the DNA extraction lab was not only to inform students on how DNA is present in humans and all organisms‚ but to also educate them on how DNA can be extracted using common household materials. Also‚ the lab was very efficient as it introduced the students to extracting their own DNA found on their cheek cells as well as letting them take an observation on how DNA appears or how it is formed. Additionally‚ students were instructed through a very clear and simple procedure‚ which guided
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Enzymes are proteins or nucleic acids that catalyze reactions. They are able to speed up reactions by reducing the activation energy of a reaction. Each kind of enzyme has a specific shape that matches its substrate so it can bind to its active site. Enzymes convert their substrates into a product. Enzyme activity are affected by factors such as temperature‚ pH‚ and time. If an enzyme is exposed to extreme heat‚ it will become denatured‚ that is‚ to become deformed and lose its original shape which
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Enzyme assay lab report Health and safety: 4-nitrophenol is harmful. Introduction: Enzymes are quaternary structured proteins that are specific biological catalysts that speed up a reaction without being used up. They contain an active site that allows substrate to bind to a specific area on the enzyme which is of a complimentary shape of the substrate. There are two models of enzyme action‚ the Lock and Key model and the Induced Fit model. The Lock and Key model states that the enzyme has a specific
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