Experiment 4 – Effect of Temperature on Enzyme Activity Aim To study the effects of temperature on the activity of amylase enzyme on starch solution. Introduction Enzymes are widely known as biological catalyst. Almost all cellular reactions are controlled and guarded by enzymes. Virtually every metabolic reaction which takes place within a living organisms are catalyzed by enzymes. Enzymes are complex three-dimensional globular proteins. Some of the enzymes are built up off proteins and
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Chemistry Coursework Rates of Reaction Investigation Introduction In this investigation I am going to be investigating the reaction between marble chips and hydrochloric acid‚ and to see what differences in the rate of this reaction arise when different concentrations of acid are used. Below is the word equation for this reaction: Calcium Carbonate + Hydrochloric Acid = Calcium Chloride + Carbon Dioxide + Water Below is the fully balanced chemical equation for this reaction. CaCo3 + HCl
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1. Prepare a lactase enzyme solution by dissolving one lactase enzyme tablet in 200 ml of water in a clean 250 ml beaker. Stir until the tablet has dissolved. Use labeling tape to label the beaker: “Lactase Enzyme Solution.” 2. Prepare a “denatured” enzyme solution by pouring 20 ml of your enzyme solution into a heat resistant tube. The test tube must have the words “Kimax” or “Pyrex” on it. If it does not‚ it is not heat resistant and may break! Use labeling tape to label the test tube: “Denatured
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Enzymes lower the amount of energy needed in a chemical reaction. This happens because enzymes are catalysts‚ so they speed up the activation rates that occur in living organisms. Without enzymes‚ it would be difficult to break down particles like food in the digestion system. Enzymes are all very specific to what chemical reactions they will work with‚ and the temperature‚ pH‚ and salt concentration have to be a specific levels in order for the enzyme to function. The structure of each enzyme has
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Coursework-Rates of Reactions Introduction Investigating how the concentration of sodium-thiosulphate (STS) and hydrochloric acid (HCL) affects the rate of reaction in the experiment. In addition I am also checking how different variables affect my experiment and also how I can make my test more precise‚ reliable and accurate. Strategy According to my results‚ as the concentration of STS solution increased the rate of reaction of my experiment was increased. Also as the concentration of STS
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Light Reactions Lab This lab explores the effect of light intensity and light wavelength on photosynthesis. The quantity of energy [ATP] produced will change depending upon on these parameters. Use "Johnson Explorations: Photosynthesis" located at http://www.mhhe.com/biosci/genbio/biolink/j_explorations/ch09expl.htm Examine the diagram before clicking "skip intro" so you have an idea what is going on. The animation show the movement of photons‚ electrons‚ water‚ protons‚ ATP etc. relating to
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Effect of temperature on enzyme activity In this experiment… Independent variable: Temperature of the amylase Dependent variable: Enzyme activity which is measured by the time for disappearance of starch Controlled variables: Volume of amylase; volume of starch solution; concentration of amylase; concentration of starch solution Prediction of results i) At low temperature‚ the rate of amylase activity is very low. ii) At optimum (=best) temperature‚ the rate of amylase activity is the
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Purpose: To determine the effect of various factors on the rate of reaction between an enzyme andits substrate‚ and also to determine the optimal ranges under which the enzyme activity ismaximized. Also to determine whether saline and alcohol are inhibitors or activators Hypothesis: PH factor prediction: I predict that as the pH increases so the activity of the enzyme willincrease until it reaches optimum pH range (pH 7) because the enzyme is less denaturedwhen it reaches the preferred pH level
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Ayaan Nadeem SBI 4UO-A Tuesday‚ February 27‚ 2013 Ms. Balmer Pineapple Jelly Enzyme Lab Discussion After completing the Pineapple Jell-O Enzyme lab‚ the final results were that the canned pineapple formed the jelly while the fresh pineapple did not. Pineapple In order for this to have occurred‚ there has to be a comparison between fresh and canned pineapple in terms of their physical and chemical properties. The physical properties of fresh pineapple are that it is sweet‚ ripe and raw.
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Principles of Biology Lab Exercise Enzymes: Catalysts of Life Instructor: Professor Alcendor By Shahid Rana Date: March 7th‚ 2013 Abstract: In this experiment we have demonstrated the function of enzymes. The whole experiment was devoted to understand how enzymes work as a catalysts and increase the chemical reaction without being used themselves. In general‚ enzymes are proteins that function as biological catalysts. These enzymes adhere to lower to amount of energy required for
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