Thin Layer Chromatography Introduction Thin Layer Chromatography or TLC is a technique used as a separation and identification technique. There are many forms of chromatography‚ but one thing that remains constant throughout all of the types of chromatography is that there is a stationary phase and a mobile phase. In the case of TLC the stationary phase is the silica gel on the TLC tray. Procedure Chromatograph method is a method of separating mixtures of two or more compounds. Two phases
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comparison with chromatograms of aspirin‚ acetaminophen‚ ibuprofen‚ mefanemic acid‚ and caffeine through the use of UV chromatography then the Rf value was calculated for each solvent. INTRODUCTION In this experiment‚ thin-layer chromatography (TLC) will be used to determine the composition of various analgesics. Chromatography is a technique used to separate and identify individual components in a mixture. Chromatographic techniques make use of the fact that components of a mixture tend to move
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University of Santo Tomas‚ Manila‚ Philippines Abstract The group used five different analgesics in this experiment: aspirin‚ acetaminophen‚ ibuprofen‚ caffeine‚ and mefenamic acid. Six analgesics were spotted on the TLC plate including the unknown. After the development of the TLC plate‚ it was placed under ultraviolet light for the spots resulted to be traced. The distance from the origin to the solvent front and the distances from the origin to the center of each spot formed were measured to calculate
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Title Identification of Analgesic Compounds Using Thin Layer Chromatography Abstract The objective of laboratory experiment six was to identify an unknown analgesic tablet using Thin Layer Chromatography (TLC) analysis. The procedure involved preparing TLC plates‚ solubilizing standard analgesics (ibuprofen‚ aspirin‚ acetaminophen‚ caffeine) and the unknown in isopropyl alcohol (IPA)‚ and developing the plates in acetone. Rf values were calculated for each compound. The unknown tablet showed an Rf
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The Relationship between the Genotype and Phenotype of Drosophila melanogaster Eye Pigment Mariam Rahmanyar 100486882 T.A: Zahra Mortaji BIOL 2020 Results: Our TLC plate showed all colours expressed by the pteridine pigments for the wild-type Drosophila as expected (Figure 1). The sample A mutant which was a bright-red eye mutant also expressed the same pigments as our wild-type. Compared to the wild type‚ sample A pigment spots were lower intensity in colour under the UV
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This experiment involved a substitution reaction to perform synthesis of 2-phenylethanol to get (2-bromoethyl)benzene. Using NaBr in an acidic solvent of H2SO4 with H2O present‚ this synthesis was possible. Subsequent evaluations using TLC and then analyzed samples by Gas Chromatography were done to evaluate the results. Using SN2 reactions‚ primary alcohol is going to be converted to alkyl halide. Since the reaction can be reversed‚ the strong sulfuric acid was to make sure the product would indeed
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acceptance and availability in the market. Furthermore‚ this study applies thin layer chromatography (TLC) technique since it is the simplest‚ cheapest yet most effective approach in terms to the study’s objectives. The TLC method implies Rf (Rate of flow) values where it is given by the distance from origin to center of subtance spot over the distance from origin to solvent front. As a result‚ the TLC analysis of Biogesic® yields the following Rf values where the given distance from origin to solvent
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Experiment 5 Title : Thin Layer Chromatography Objectives: i. To distinguish polar and non-polar solvents. ii. To familiar with the analysis technique by using the thin layer chromatography. iii. To differentiate the retention factor‚ Rf for different compounds. [pic] Result: |Compound |Distance traveled by the compound | |o-nitroanaline |2.45
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Topic 8a – Carbonyl compounds Revision Notes 1. Introduction • Aldehydes and ketones are carbonyl compounds • They contain the carbonyl group C=O • The functional group in aldehydes is –CHO on the end of a chain e.g. ethanal CH3CHO • The functional group in ketones is C=O not at the end of a chain e.g. propanone CH3COCH3 2. AS Recap • Primary alcohols are oxidised by acidified potassium dichromate. An aldehyde is produced first and this can be further oxidised
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resulting in the formation of a hydroperoxide and eventually a ketone (after removal of water). Since the goal was to have a mixture of 50% fluorene conversion‚ Thin Layer Chromatography (TLC) was carried out several times to assess the rate of the reaction. In order to separate the aqueous composites from the solution
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