overdose‚ people also overdose on common over-the-counter (OTC) drugs‚ like aspirin‚ when attempting to take their own lives. Thin-layer chromatography (TLC) is one technique used to identify unknown drugs. Chromatography is simple to perform‚ is straightforward to interpret‚ and works equally well for legal and illegal substances. The experiment uses TLC to identify the active ingredients in some common OTC painkillers. Introduction: The majority of evidence submitted
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the separation. Thin layer chromatography (TLC) is a chromatographic technique that we use to separate the components of a mixture using a thin stationary phase such as silica plate supported by an inert backing. It applies mainly in medical‚ biological‚ chemical and pharmaceutical sciences because it is easy to be done and saves time because the reaction is fast. This method used for identifying mixtures by separating the compounds in the mixture. TLC is also used to help determining the number
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recorded in lab. However‚ spot results were recorded. The TLC plates 1-3 showed the presence of starting material (SM) in the crude product (CP) lane. TLC plate 1 was taken after adding the initial addition of 0.25mL of bleach. TLC plate 2 was taken after adding an additional 0.08mL of bleach because plate 1 still showed presence of SM in the CP lane. TLC plate 3 was taken after adding an additional 1mL of bleach for the same reason. However‚ TLC plate 4 did not show any SM in the CP lane and this was
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Acetylsalicylic acid is the active pharmaceutical ingredient in aspirin and can be synthesized by the esterification reaction of salicylic acid and acetic anhydride in the presence of an acid catalyst. An esterification reaction is when an acid is converted into an ester by combining with an alcohol and removing a water molecule. When heating the salicylic acid mixture in the warm water bath‚ the mixture should be removed from the bath within 8 minutes‚ to reduce the chance of the acetylsalicylic
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ISOLATION OF TRIMYRISTIN FROM NUTMEG ABSTRACT The purpose if the experiment was to extract the Trimyristan from ground nutmeg using extraction‚ distillation and recrystallization procedures. This experiment involves extraction and separation techniques that yield a purified product. The product was extracted and a distillation process was used to remove any remaining solvent. The recrystallization of the sample in turn produced a pure sample‚ which was proven using the known melting
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different kinds of functional groups and number of functional groups of molecules will determine the separation. Depending on what kind of solute‚ solvent‚ and adsorbent used‚ molecules will experience the interaction during dynamic equilibrium. On a TLC plate‚ capillary tube is used to transfer o- and p- hydroxyacetopheone‚ taking advantage of capillary force to make small spotting. A 30:70 ether acetate: petroleum ether is used as a solvent‚ which will not interact with surface of the silica gel due
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Prepare a TLC chamber by cutting a large circle of filter paper in half and placing it in a beaker. Add ~ 6 mL of methylene chloride (or 30% acetone in heptanes-needs to be fresh‚ otherwise acetone will evaporate) and cover the beaker with a watchglass. For each TLC run‚ you will need a silica gel plate and two clean capillary tubes for spotting the plate. Draw a horizontal line near the bottom of each TLC plate (the line must be higher than the level of solvent in the TLC chamber). Draw three
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analyze the components of the spinach extract. This was done using a TLC plate and spotting each of the components on to the plate as well as spotting the standard spinach extract. A beaker was used with the developing solvent‚ filter paper‚ and TLC plate inside and a watch glass over top of the beaker to perform the thin layer chromatography. After the solvent front (30% ethyl acetate in hexane) reached about an inch from the top‚ the TLC plate was removed from the beaker and the developing spots were
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aspirin collected. d. Weight of unknown compound isolated e. Weight of recrystallized unknown compound f. Melting point range of recrystallized unknown compound. g. TLC sketches with appropriate data for Rf calculations (Clearly label each column of spots on the page so I know what it is) h. Mixed melting point based on information from TLC Final Report
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Preparation of an Ester Acetylsalicylic Acid (Aspirin) OBJECTIVE: To become familiar with the techniques and principle of esterification. DISCUSSION: Aspirin is a drug widely used as an antipyretic agent (to reduce fever)‚ as an analgesic agent (to reduce pain)‚ and/or as an anti-inflammatory agent (to reduce redness‚ heat or swelling in tissues). Chemically‚ aspirin is an ester. Esters are the products of reaction of acids with alcohols‚ as shown in the following equation using
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