reasons; detecting infections‚ diseases‚ and particular proteins in a tissue. When western blotting‚ first the proteins are extracted and undergo Gel Electrophoresis‚ next it goes through elecroblotting‚ and finally detects the proteins. When the results after every step is completed‚ the membrane is analyzed based on where the bands are present in the gel. It has been told that actin and myosin is present in fish‚ making it tougher to chew in most cases. To test the theory of actin and myosin being
Premium Molecular biology Western blot Gene expression
Examining the RNA Interference Mechanism in the dpy-13 Gene in C. Elegans Through Feeding Mehdi Misto Lab: Monday 1:00 – 4:50 PM 11 December 2012 Introduction: RNA interference‚ or RNAi‚ is a biological process in which RNA molecules reduce the gene expression of an organism. This is done typically by causing the destruction of specific mRNA molecules. RNAs are direct products of genes‚ these small RNAs can bind to other mRNA molecules to either increase or decrease their activity like in the example
Premium RNA DNA
Genetically Modified Organisms INTRODUCTION: The purpose of this lab was to identify if non-labeled food products are actually genetically modified foods. Before we could begin testing this theory we first had to gain an understanding about genetically modified organisms in general. This was rather easy because if you have been to any grocery store lately you have without a doubt seen products with labels saying "GMO-free" or even "contains only non-GMO ingredients." GMO actually stands for
Premium Polymerase chain reaction DNA Molecular biology
Polyacrylamide gel electrophoresis is then used to seperate and analyse the polynucleotide chains in sizes ranging between 15 and 1000 nucleotides on the basis of size. The four mixtures of radiolabelled polynucleotides are transferred into separate wells according to the nucleotide type. The nucleotides are run through the gel at a voltage of 1500 volts. Due to the fact that DNA strands are negatively charged they are attracted to the positive anode and therefore move down the gel towards the anode
Premium DNA Gene Genetics
CHROMOTOGRAPHY Chromatography is used to separate mixtures of substances into their components. All forms of chromatography work on the same principle that they all have a stationary phase (a solid or a liquid supported on a solid) and a mobile phase where liquid or a gas is involved. The mobile phase flows through the stationary phase and carries the components of the mixtures with it. Different components travel at different rates. In paper chromatography‚ the stationary phase is a very uniform
Premium High performance liquid chromatography Chromatography Analytical chemistry
and Wang 2011). The first objective of this experiment was to use two restriction endonucleases‚ EcoR1 and Xba1‚ to cut pUC19 and pBK-CMV. To ensure that the plasmids were successfully cut‚ analysis of the plasmid was carried out using gel electrophoresis. Gel
Premium DNA Molecular biology
DNA FINGERPRINTING DNA fingerprinting is a method of identification that compares fragments of deoxyribonucleic acid. It is a technique used to distinguish between individuals of the same species by using only samples of their DNA. It is also called DNA typing. DNA is the genetic material found within the cell nuclei of all living things. In mammals‚ the strands of DNA are grouped into structures called chromosomes. Unless dealing with identical twins‚ the complete DNA of each individual is unique
Premium DNA
460 basepair fragment of DNA from within the control region of the mitochondrial genome. This can be done using three water baths or‚ if one is available‚ a thermal cycler (PCR machine). After it has been amplified‚ the DNA is run on an electrophoresis gel. Note: This method has been adapted from one developed by the Dolan DNA Learning Center at Cold Spring Harbor Laboratory. More details are available from the DNA Learning Center’s Web site: http://vector.cshl.org/geneticorigins Equipment
Premium Polymerase chain reaction
The wild type and the mutant type strains of Halobacteria were used in this experiment to examine the phenotypic differences against each strain’s genotypes. The mutant strain (KBT-1) did not possess gas vesicles‚ which decreased its ability to float to the surface. The lack of gas vesicles in the mutant strain made the colonies a red color. The wild type strain (NRC-1) had gas vesicles and appeared a pink pigment color. Growth on the agar allowed one to examine the specific colonies. Inoculation
Premium Bacteria Microbiology DNA
enzyme digestion in DNA molecules of different individuals. Diverse mutations that might have occurred affect DNA molecules in different ways‚ producing fragments of variable lengths. These differences in fragment lengths can be seen after gel electrophoresis‚ hybridisation and visualisation. Isolating DNA Isolating DNA is the first step for many DNA-based technologies. DNA is found either in nuclear chromosomes or in organelles (mitochondria and chloroplasts). To extract DNA from its location
Premium DNA Molecular biology