making changes in the DNA code of a living organism‚ working almost the same. It has many purposes including clones‚ perfect humans and cures for genetic diseases. Gel electrophoresis is a powerful tool used for separation and analysis of macromolecules and their fragments‚ based on their size and charge. You place the DNA in the gel. DNA is a long‚ strand-like molecule where genes are written in genetic code. Extracted enzymes recognize and extract the DNA at a specific sequence. Transgenic
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A. Fermentation Lab- The basic process Prepared 3 beakers with contents listed below. ( a. Beaker 1: glucose only b. Beaker 2: Starch only c. Beaker 3: Starch + amylase). Poured contents of each beaker into its respective fermentation tube‚ ensuring the tail portion of the tube was filled with liquid. Placed tubes in an incubator at 37 degrees‚ measuring distance between tip of tube tail to fluid level at 20‚ 40‚ and 60 minute intervals. Calculated gas volume using this distance along with radius
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control plant was digested with EcoRI‚ and then separated by electrophoresis on 0.8% agarose gel. Digestion profile was checked with 1µg DNA‚ with single cutter enzymes EcoRI (10U) in T-DNA region. For southern experiment‚ 20µg DNA was digested overnight. Restricted fragments were separated on 0.8% agarose gel at 40V for 8 h. De-purination (0.25N HCl) was done in glass pot for 15 min with shaking at room temperature. Subsequently rinse the gel in autoclaved distill water two times for 10 min interval
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Name ______________________________________ Virtual Lab Report: Part I Due by: 11:59 PM PST on the second Saturday of class Virtual Lab 1: Virtual Microscopy A. Estimate the size (length and width) of these microscopic objects in micrometers (microns): 1. An E. Coli cell. 3 x 0.6 um =1.8 um 2 A mitochondrion. 4 x 0.8 um = 3.2 um 3. A Red blood cell. 8 um 4. A virus. _Hepatitis 45 nm = .045 um 5. A water molecule. 275 pm =.275 um B. 1 Describe three differences between prokaryotic and eukaryotic
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Most lipid-anchored proteins are attached to the inner side of a plasma membrane by covalent linkage to a fatty acid or prenyl group. • A useful technique for studying membrane proteins is SDS-PAGE ( sodium dodecyl sulfate- polyacrylamide gel electrophoresis). In this method‚ the role of SDS is to coat the proteins with a negative charge. • Lectins are proteins that bind sugars and have been useful in the study of glycoproteins. • You discover an integral membrane protein that has amino acid residues
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handle the microbiological components of these processes. In this study‚ the composition and structure of microbial communities in acid mineral bioleaching systems have been studied using a PCR-based cloning approach. Denaturing gradient gel electrophoresis (DGGE) analysis of PCRamplified 16S rRNA gene fragments from bacteria was used to evaluate the changes in the bacterial community in the process of chalcopyrite bioleaching in a shaken flask system. The results revealed that the bacterial
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infection. The agglutination test involves taking the culture of antigens mixed with antibodies and examining it under a microscope to see if clumping occurs. The precipitation test determines the similarity of antigens. The antibodies are placed in agar gel with the antigens. A line forms where the two interact. Antigens are microorganisms that have the potential to cause infection in the body. When the body is exposed to an antigen‚ it produces antibodies that are designed to fight the specific antigen
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University‚ Savar‚ Dhaka 1342‚ Bangladesh Abbreviations: Endo H‚ Endoglycosidase H; PR protein‚ pathogenesis related protein; P. pastoris‚ Pichia pastoris; VTS‚ vacuolar targeting signal; PCR‚ polymerase chain reaction; PAGE‚ polyacrylamide gel electrophoresis; SDS‚ sodium dodecyl sulfate Abstract A yam (Dioscorea opposita Thunb) class IV chitinase‚ whose genomic DNA was cloned by Mitsunaga et al. (2004)‚ was produced by the recombinant Pichia pastoris X-33 yields remarkably‚ such as 66 mg/ L of
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Polymerase chain reaction The Polymerase chain reaction (PCR) is a biochemical technology in molecular biology to amplify a single or a few copies of a piece of DNA across several orders of magnitude‚ generating thousands to millions of copies of a particular DNA sequence. Developed in 1983 by Kary Mullis‚ PCR is now a common and often indispensable technique used in medical and biological research labs for a variety of applications. These include DNA cloning for sequencing‚ DNA-based phylogeny
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DNA Profiling and Ethics Reflection Journal Vanshika Khemka 14th October 2012 "33 autorad off" On 10th September 1984‚ geneticist Alec Jeffrey’s wrote these three words in his red desk diary. This marked the completion of an experiment‚ which studied how inherited illnesses pass through families. The experiment failed entirely. (McKie‚ 2009) However‚ this led to the most profound discovery: the world’s first DNA fingerprint. Now‚ the smallest swab of blood or sweat can determine
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