“Enzyme X is a highly pigmented protein that imparts the characteristic color to certain blue-green algae. It also facilitates a reaction necessary to the survival of this species; we can follow the kinetics of this reaction by measuring the conversion of Substance X to Substance Y at various times during purification.” Techniques and methodologies for extracting the proteins and purifying Enzyme X are as follows: The blue-green tinted proteins in Enzyme X give a distinct color to algae. The proteins
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[pic] Faculty of Science Undergraduate Modular Scheme Session 2010/2011 Semester 2 Level 4 MODULE: LS1180: Genetics and Molecular Biology DATE: 31 May 2011 TIME: 09.30 – 11.30 Instructions to Candidates This paper contains THIRTY-EIGHT questions in FIVE sections: Sections A‚ B‚ C‚ D and E. Answer THIRTY-FOUR questions only Section A: THIS SECTION HAS BEEN WITHHELD BY THE FACULTY Answer ALL THIRTY questions on the MCQ sheet provided Section
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lysis was present; therefore‚ P. larvae was isolated. Isolating DNA from the Pure Colonies Grown In addition‚ DNA were isolated from the colonies grown and its concentration was 33.6 ng/uL and the A260/280 value was 1.84. Next‚ an agarose gel electrophoresis was conducted to see if any DNA fragments were present. A band was visible and that validates that genomic DNA has been isolated. PCR Amplification to Determine Strain of P. larvae with ERIC-Specific Primers ERIC-specific primers were used
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identify even very low levels of bovine presence in the sample. According to the study done by Ilhak and Arslan (2007)‚ it was identified that DNA concentrations of even 0.1% of the sample can be detected and amplified via PCR and can be visualized on gel
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AP Biology Midterm Study Guide Ecology‚ Biochemistry‚ Cells‚ Genetics‚ and Evolution Midterm Date: Part I: Multiple Choice Questions (60) VOCAB TO KNOW: Element Diffusion Homozygous Lytic Cycle Compound Isotonic Heterozygous Lysogenic Cycle Neutrons Hypertonic Phenotype Transformation Protons Hypotonic Genotype Transduction Electrons Osmosis Codominance Conjugation Atomic Number Turgid Wild type Plasmid Mass Number Flaccid Mutant Evolution
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Genes Review 1) Selective breeding – chosen based on what can be seen and detected (favourable phenotype) Genetic engineering – selected based on genes 2) - DNA are found in nucleus (where most DNA are found)‚ mitochondria‚ chloroplast 3) - Translation requires ribosome‚ mRNA‚ tRNA (anticodon) and amino acids - DNA template‚ polymerase for transcription 4) Mitosis generates daughter cells that are identical Meiosis – homologous recombination 5) DNA coding sequence: 5` GGGCCCTTTAAA
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supercoiled inside the cell‚ isolation of chromosomal DNA almost always leads to breakage of the strands and consequent loss of supercoiling. Separation can then proceed by a variety of techniques‚ including ultracentrifugation and electrophoresis on agarose gels. Among the most widespread and well-studied groups of plasmids are the resistance plasmids which confer resistance to antibiotics and various other inhibitors of growth. Today‚ there are are number of well established techniques commercially
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Unit 5 Biology notes Contents Section 9.1 – Sensory Reception 3 Section 9.2 – Nervous Control 4 Section 9.3 – Control of heart rate 5 Section 9.4 – Role of receptors 6 Section 10.1 – Coordination 8 Section 10.2 – Neurons 10 Section 10.3 – The nerve impulse 11 Section 10.5 – The speed of a nerve impulse 12 Section 10.6/10.7 – Structure and function of the synapse / Transmission across
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prioritize‚ or coordinate biological lab activities. Molecular biologists must prepare reports‚ manuscripts‚ and meeting presentations. Molecular biologists also perform laboratory procedures following protocols including cloning and extraction‚ gel electrophoresis‚ ribonucleic acid purification‚ or deoxyribonucleic acid sequencing. Salary Range: The average median wage for a molecular biologist in 2011 was $70‚790. The projected growth of molecular biologists from 2012-2020 is expected to be around
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Chromatography (HIC) is used to purify the foreign protein. Protein gel electrophoresis is used to check and analyze the pure protein. Research scientists use Green Fluorescent Protein (GFP) as a master or tag to learn about the biology of individual cells and multicultural organisms. This lab introduces a rapid method to purify recombinant GFP using HIC. Once the protein is purified‚ it may be analyzed using polysaccharide gel electrophoresis (PAGE). Purpose: To illustrate the process of transformation
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