Principles of Biology Lab Exercise Enzymes: Catalysts of Life Instructor: Professor Alcendor By Shahid Rana Date: March 7th‚ 2013 Abstract: In this experiment we have demonstrated the function of enzymes. The whole experiment was devoted to understand how enzymes work as a catalysts and increase the chemical reaction without being used themselves. In general‚ enzymes are proteins that function as biological catalysts. These enzymes adhere to lower to amount of energy required for
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I. Title. Restriction Enzyme Mapping of pBR322 Using Agarose Gel Electrophoresis. II. Authors. Author: Partner: Section: Thursday‚ 1:10 pm Date of Experiment: October 25‚ 2012 III. Introduction. Restriction enzymes (or restriction endonucleases)‚ originally isolated from Haemophilus influenzae in 1970‚ are enzymes within a cell that cleave foreign DNA within a specific and predictable nucleotide sequence (known as a restriction site) regardless of the source of such DNA. Such restriction
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Exploring Enzymes - Ground-Up Tissue Activity Abstract Our experiment looked at how increasing the surface area of a substance affects the amount of bubbles created due to the presence of the enzyme catalase. The experiment used two pieces of fish‚ one whole and one ground up‚ which were then covered in hydrogen peroxide. This method allowed us to observe the catalase in ground up fish break down the hydrogen peroxide at a quicker rate than in the piece of fish left intact. This was determined
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Enzyme Catalysis Maltose sugar is broken apart by maltase enzyme Substrate are molecules enclosed in the enzyme Catalase: found in every living thing Takes two molecules of hydrogen peroxide and converts it irreversibly to create oxygen gas and water 2H2O2O2+2H2O Question: What variable affects the rate of enzyme catalysis most? Variables Tested: Hydrogen Peroxide concentration‚ yeast concentration‚ heat and pH Materials: 10% glucose mixture 1.5 %‚ 3% and 6% peroxide mixture Yeast
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Review for Bio 103 Lab Midterm Read the lab manual and study your worksheets. Vocabulary: Metric system‚ percent error‚ systematic error‚ random error‚ accuracy‚ precision Cylinder‚ beaker‚ pippette‚ balance‚ meniscus Field of view‚ inversion‚ total magnification‚ depth of field‚ par focal Microscope parts: eye piece‚ body tube‚ arm‚ nosepiece‚ objectives‚ stage clips‚ mechanical stage‚ diaphragm‚ condenser‚ fine and course adjustments‚ light stage‚ base Protein‚ peptide bond‚ simple sugar
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In the BIO 14 Lab‚ the organismal system used for the last of three labs this semester is the Mimosa pudica plant. In these three weeks the stimulus-response of these plants will used to conduct experiments on the phenotypic plasticity of Mimosa pudica. Phenotypic plasticity is the ability of an organism to change its phenotypic traits in response to changes in the environment. And while very beneficial in some ways such as by allowing organisms to adapt to a changing environment quickly‚ it also
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Environmental Systems Bio-cylinder Lab Report Planning: The question that we‚ as a class‚ were trying to answer was: How long can the organisms survive within a sample-closed ecosystem? To answer this three separate classes had the opportunity to create their own bio-cylinders‚ place a specified number of organisms‚ seal it‚ and observe the cylinder for however long the organisms were able to survive. Each class made small modifications to the number of fish and snails that placed within the
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The enzyme tyrosinase was successively extracted by combining a homogenate of a potato and sodium sulfate with ammonium sulfate. Tyrosinase was successfully extracted by taking advantage of solubility properties of certain proteins. A standard curve was generated indicating dopachrome absorbance values through the use of a spectrophotometer and a computer graphing program. A spectrophotometer was used to measure either the amount of light that passed through a solution (transmittance) of the amount
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string to a 50 g weight hanger and drape the string over the pulley. The string length should be such that‚ when one hanger hits the floor‚ the upper part of the other hanger is near the pulley‚ without touching the pulley. (You may find that the lab assistant has already set up the apparatus as described here. If so‚ double check the setup.) 2. Place equal masses of approximately 1000 g on each weight hanger. These masses should include four 5 g masses at the top of the left hanger. Hold back
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Reminder: All post labs need to be 1- typed (not handwritten) ‚ 2- original (not copied from a classmate)‚ 3- answered using complete statements and 4- turned in at the beginning of the lab. Post-lab questions for Topic 5 – Enzymes Name: Date: Group: T W R Formation and Detection of Benzoquinone Table 1. Formation and Detection of Benzoquinone: Record Absorbance Time 2A-Potato extract + cathecol 2B- Potato extract + water 2C- Catechol + water After 10 min 1- What were the substrate
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