CHAPTER I INTRODUCTION 1.1 Background Food is one of human sources of calorie‚ protein‚ fats‚ and nutrition. Yet‚ because of the highly nutritious content‚ food is susceptible to growth of microorganisms. By the presence of microorganisms in food‚ the food is more likely to have shorter shelf life. Thus‚ mostly it is resolved by the addition of antimicrobial substances to food‚ such as condiments and preservatives. Condiments and preservatives could inhibit the growth of microorganisms
Premium Bacteria Microbiology
Sarcina lutea‚ Pseudomonas fragi‚ Micrococcus luteus‚ Alcaligenes faecalis‚ Clostridium sporogenes‚ and Micrococcus roseus. There were several qualitative tests that could be conducted to determine the identity of the unknown species‚ for example‚ Gram staining‚ Fermentation‚ Catalase‚ Oxidase‚ Starch Hydrolysis‚ Litmus Milk‚ MOI medium‚ and the Gelatin Test. All tests and techniques used were performed in accordance with The Microbiology Lab Manual. Materials and Method: A stock broth culture of
Premium Bacteria Microbiology Escherichia coli
produce a magnified image? How does a lens produce a magnified image? 3) What are some ways to increase contrast in microscopy? 4) Define: simple stain‚ differential stain and special stain. 5) What are the steps in a gram stain? 6) How can staining aid in the diagnosis of TB? 7) In general‚ can viruses be imaged with a light microscope? 8) Are all bacteria microscopic (beyond the ability to see with the naked eye)? Prokaryotic structure and function 1) What
Premium Bacteria
The cell wall maintains the integrity of the cell. The cell wall helps protect the cell against the environmental changes it it allows most molecules to pass through it. This layer is composed of molecules called peptidoglycans. Just inside the cell wall is a less rigid envelope called the plasma membrane also known as the cytoplasmic membrane. In bacterial cells the plasma membrane has two primary functions: it serves as a selective barrier to molecules that are penetrating the cell wall allowing
Premium Bacteria Cell Cell membrane
Exercise 5 Introduction: Many different species of bacteria look similar under the microscope and also have the same staining results (ex. Gram stain). To be able to differentiate between the different species‚ one can look at the metabolic differences (fermentation)‚ as well as the environmental condition differences (temperature‚ pH‚ oxygen requirements). Being able to manipulate these conditions in a controlled environment can help to correctly identify the exact bacteria. Different media
Premium Bacteria Microbiology Gram staining
Question 1 | | 1 / 1 point | Microbes are not responsible for ________. | | synthesis of acetone and alcohol | | | breaking down wastes | | | synthesis of vitamins | | | antibody production | | | oxygen generation via photosynthesis | View Feedback | | Correct! | Question 2 | | 1 / 1 point | The cell theory states that ________ are made up of cells. | | protozoa | | | archaea | | | plants | | | all living things | | | animals | Question
Premium Bacteria
categories‚ such as‚ morphology‚ physiology‚ antibacterial susceptibility‚ selective media‚ and biochemical provide results. Both the unknown isolate and members of the Micrococcus genus were shown to be obligate aerobes. By using staining methods‚ this proved that the organism is gram positive. Morphology‚ such as‚ orange pigmentation and coccus shape provide similarities to the Micrococcus genus. Physiological tests were shown to be obligate aerobe‚ mesophile‚ neutrophile‚ and osmotolerant organism. Biochemical
Free Bacteria
MacConkey Agar test This test is used for isolating the lactose fermenting bacteria from lactose non-fermenting bacteria while inhibiting the growth of gram positive bacteria. The lack of growth from my bacteria indicates that my bacterium has negative result. Mannitol Salt Agar This test is used to observe the difference between gram positive staphylococci bacteria and to differentiate for mannitol fermentation. If a yellow media change is observed then the bacteria has the presence of
Premium Bacteria Gram staining Staining
The tests used to confirm the fermentation were a gas production test‚ pH test‚ Gram stain‚ and turbidity test. The results showed during the conversion of milk to Kefir there was gas produced‚ a decrease in pH‚ more bacteria present‚ and an increase in absorbency. The results proved that fermentation occurred with three positive confirmatory results from the gas production‚ pH‚ and turbidity tests‚ while the Gram stain test acted as a negative confirmatory test. Introduction: The main goal of
Premium Carbon dioxide Kefir Gram staining
purpose of this lab is to: 1. Collect a sample of soil from (your school) on October 27th 2011 and collect a sample of pond water from (the name of your lake or pond) on October 27th and record the data results for both samples. 2. Using the Gram stain‚ and light microscope identify at least 2 Prokaryotes (bacteria) found in the water samples that are isolated on the MacConkey agar plates and the nutrient agar plate. Using the Identification Lab manual‚ identify at least 2 Eukaryotes (fungus)
Premium Gram staining Bacteria Fungus