colored colony. Gram-staining was utilized as a first step to differentiate between gram-positive and gram-negative. After determining that it was a Gram negative single bacillus‚ biochemical tests performed and their purpose and results are shown in table and flow chart form. Table 1: biochemical Test Results Test | Purpose | Reagents | Observations | Results | Gram stain | To determine the Gram reaction of the bacterium | Crystal violet‚ Iodine‚ Alcohol‚ Sarasin | Pink rod | Gram-negative rods
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Having the ability to collect and identify unknown microorganisms is vital in health and medicine. This capability is important for a variety of reasons‚ such as knowing the causative agent of disease‚ knowing if the microorganism obtains any beneficial properties and knowing the correct microorganism to use to create a successful antibiotic. Implementing the experimental methods learned thus far in the microbiology laboratory allowed an unknown bacterium to be identified as a result of this study
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The catalase test is used to differentiate staphylococci (catalase-positive) from streptococci (catalase-negative). The enzyme‚ catalase‚ protects the bacteria from the toxic by-products of oxygen metabolism. This enzyme is produced by bacteria that respires using oxygen. The catalase-positive bacteria include strict aerobes. Catalase-negative bacteria may be anaerobes‚ or they may be facultative anaerobes do not respire using oxygen as a terminal electron acceptor. The test reaction is very fast
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taken out of the Dixie cups and were individually massed again. The resulting data is as follows. Change in Mass Over 24 Hours Variable A B Q X Y Z Original Mass 1.93 grams 2.04 grams 2.20 grams 2.27 grams 2.73 grams 2.58 grams “After” Mass 1.55 grams 1.51 grams 2.35 grams 2.90 grams 1.88 grams 2.41 grams % Change -20 % -26% +7% +28% -31% -7% Percent Change in Concentration Variable Concentration X 0 Q 0.2 Z 0.4 A 0.6 B 0.8 Y 1.0 As the concentration
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dish and record the mass in grams. Add salt to the petri dish until the mass is about 75 g. Record the mass to the nearest tenth of a gram. Use the scoopula to add a small amount of salt from the petri dish(about 5 grams) to the 250 mL of water. Stir the salt in the water with a glass stirring rod until completely dissolved‚ about two minutes. After the previous salt has been dissolved(no settling at the bottom of the beaker)‚ add more about 5 more grams of salt and repeat step number
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athletes need 0.5-0.75 grams of protein daily for every per pound of body weight Competitive athletes need 0.6-0.9 grams per pound Teenage athletes need 0.8-0.9 grams per pound Athletes building muscle mass need 0.7-0.9 grams per pound The maximum amount of protein that most adults can use per day is 0.9 grams per pound of body weight. So if you’re an adult athlete who wants to build muscle mass‚ and you weigh about 175 pounds‚ the most protein you would need per day is 157.5 grams. That sounds like a
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characteristics. Afterwards‚ a Gram stain was performed from a sample of the agar plate and the slide was viewed under the microscope. Once‚ the microscopic visual was captured‚ a catalase test followed. Next‚ for further data‚ MSA results were recorded‚ along with antibiotic observations of demo plates. Lastly‚ with the information gathered‚ it was identified that the unknown sample was Streptococcus salivarius. Introduction: In this lab‚ the exploration of an unknown gram positive bacteria was conducted
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Trial 3 | Trial 4 | Trial 5 | Mass of cashew (in grams) | 1.00 g (± 0.01 g) | 1.00 g (± 0.01 g) | 1.70 g (± 0.01 g) | 1.40 g (± 0.01 g) | 1.50 g (± 0.01 g) | Mass of remaining material (in grams) | 0.60 g (± 0.01 g) | 0.30 g (± 0.01 g) | 0.30 g (± 0.01 g) | 0.50 g (± 0.01 g) | 0.80 g (± 0.01 g) | Mass of cashew that burned (in grams) | 0.40 g (± 0.01 g) | 0.70 g (± 0.01 g) | 1.40 g
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Michael Castelli Keegan Bauman Potato Lab Dr. Peano Block 1 Introduction: Potatoes are just your average food; they have been around for as long as the human race can remember. In this lab we will make them be a prime example of osmosis‚ which is a transport mechanism. In this lab the problem being tested was what environment affects the mass of the potatoes the most. The independent variable is the environments used salt‚ water and iodine solution. The dependent variable is the mass of
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every time and same tick rate of the ticker timer. The controlled variable will be controlled to create a fair test. Apparatus: * Power Pack * Ticker Timer * Ticker Tape * Trolley * 1 meter string * Pulley wheel * 50 gram weights x10 * Ruler * Calculator (recommended) * Sticky tape Method: To be done on bench 1. Plug the power pack into a power source. 2. Attach the power pack to the ticker timer‚ by attaching 1 plug from the black (negative)
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