Alyse Rose Microbiology Lab Bacterial Unknown March 25‚ 2013 Bacterial Unknown Report Each student was given an unknown bacteria to figure out. I was given the unknown bacteria S38. Everybody is supposed to do all sorts of test to identify the bacteria. The first thing I did was smear my bacteria on a liquid medium. I then proceeded to incubate the medium for 24-48 hours. 1. GRAM STAIN The next step I took in finding my unknown bacteria was to gram stain it. This is used to differentiate
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the formation of iminium ion which can be easily hydrolysed forming the original carbonyl group. in summary the overall acylation reaction of a carbonyl compound follows the route of first synthesising the enamine‚ the acylation and finally the hydrolysis of the iminium ion to
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4-(hydroxymethyl)phenol the second is treating the 4-(hydroxymethyl)phenol compound with NaCN in hot DMF to form the product of 2-(4-hydroxyphenyl)acetonitrile. Then 2-(4-hydroxyphenyl)acetonitrile will be refluxed with H2O and Ethanol. After the hydrolysis with water and ethanol‚ this compound will be catalysed using a Pt catalyst which forms the product of acetamide then condensed with hot piperidine gives 2-[4-(2-oxiranylmethoxy)phenyl]acetamide. Then the oxirane ring of 2-[4-(2-oxiranylmethoxy)phenyl]acetamide
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which substrates it binds with to the active site.(AS biology‚ 2008). There is a lock and key hypothesis‚ as shown in diagram 1: (http://www.skinnersbiology.co.uk/enzyme.htm) the aim of this practical is to determine the activation energy of hydrolysis of
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travels down the T-tubules of the sarcolemma‚ which then activates the release of Ca++ . The Ca++then binds to troponin triggering the movement of tropomyosin away from its blocking position at the myosin and actin binding sites. The binding and hydrolysis of ATP to the myosin head produces changes‚ causing the filament to slide against the myosin filament and the sarcomere to shorten (Johnson 37-38).
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Of HCl = 1.376 x 10-2L x 1.084×10-1molL-1 =1.492 x 10-3 moles Number of moles of A.S.A can be found by the difference between the mean number of moles of NaOH added for hydrolysis and the mean number of moles of HCl. Avg.vol=sample volumes# of samples Avg. vol = (42.83 + 43.40) 2 = 43.12 mL # of moles of NaOH = Mean vol of NaOH x Molar Conc. Of NaOH = 4.312 x 10-2L ×0.09085molL-1
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rxn‚ Wolff‚ H-based nucleophile carbonyl reduction‚ imine formation‚ reductive amination (rxn only)‚ ketal/acetal formation‚ dithiane chemistry (rxn only)‚ alpha-bromination of ketones‚ -COOH properties (the trends on HW4)‚ Fischer‚ acidic/basic hydrolysis of esters/amides/nitriles‚ CH2N2‚ acid chloride formation (rxn only)‚ acid chloride rxns with esters and amides. 2 Rxn of carboxyllic acid derivatives w/organometallic reagents‚ Cuprate preparation and rxn‚ Reduction of carboxyllic acid derivatives
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18) What is the difference between an aqueous solution and a non aqueous solution (or vehicle) (refer to pg 439 if necessary). Aqueous - preferred for injection Non-aqueous – used when there is poor drug sol. In water or a drug is susc. To hydrolysis pharmaceutical formulators will develop a nonaq. Vehicle (ex. Veg oil‚ glycerin‚ PEG) Like water‚ must not exert pharmacological activity nor adversely affect activity of drug
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Digestive Chemicals 2. What is the general function of digestive enzymes? Do most of the works in chemical digestion 3. Identify other chemicals needed for digestion. a. Water c. Bile b. Gastric acid d. Bicarbonate 4. Hydrolysis is the breakdown of larger molecules to form smaller molecules. Water molecules are used in this process. a. Define substrate. When water and enzyme are used to break down a large nutrient molecule b. Define products. Is the result of
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nExperiment C: Kinetics of the Hydrolysis of T-butyl Chloride Post-Lab Ch202 Postlab Assignment Nov 5/2014 L10 Radwan Ahmed 130770850 1. What was green about this experiment was that the solvents used (acetone and water)‚ and even the tert butyl chloride were fairly safe to handle with respect to the other chemicals used in the lab. This is why gloves were not worn‚ and one reason why this experiment follows GCP # 3 where chemical products should be designed to preserve efficacy of the function
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