coccoid forms. Bacterial morphology was determined by gram staining via light microscopy (Camera Digital DP 72-BX 51‚ Soft imaging system‚ Olympus‚ Japan). When coccoid forms of H. pylori isolates reached to more than 90%‚ bacteria went for other processing as well as evaluating their viability. The same approach was repeated for reference strain as control group with and without antibiotic. Coccoid form suspensions of H. pylori were centrifuged and washed by PBS. Then‚ bacterial sediments were stained
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How do people "catch pneumonia"? Some cases of pneumonia are contracted by breathing in small droplets that contain the organisms that can cause pneumonia. These droplets get into the air when a person infected with these germs coughs or sneezes. In other cases‚ pneumonia is caused when bacteria or viruses that are normally present in the mouth‚ throat‚ or nose inadvertently enter the lung. During sleep‚ it is quite common for people to aspirate secretions from the mouth‚ throat‚ or nose. Normally
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------------------------------------------------- ------------------------------------------------- EVALUATION OF EFFECTIVITY OF SOAPS AND DETEGENTS AGAINST BACTERIA BACTARIUM EVALUATION
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gives cells a vibrating look. This does not show proper motility. Correct motility is movement in a specific constant direction that shows winding and spinning. What morphological structure is responsible for bacterial motility? Flagella are the morphological structure responsible for bacterial motility. These are semi-rigid structures used in moving microbial cells. They are attached to the cell through intricate proteins named the hook and basal
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Review all chapters in your exercise manual beginning with the introduction INTRODUCTION: (Covered 9.4.14 II Week 1) Biosafety levels1: basic level of containment. Hand washing or wearing gloves 2: Appropriate for working with human body fluids. Autoclave‚ sharps containers‚ lab coats 3: appropriate for working with pathogens that can be transmitted via respiratory route. Self-closing‚ double doors and sealed windows 4: Highest level. Aerosol pathogens; pathogens with no vaccine/treatment. Separate
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Experiment 1- Title: Observing Bacteria and Blood Purpose: The purpose of this experiment is to learn how to use a compound microscope and an oil immersion lens while observing prepared bacterial slides. Additionally‚ it will be necessary to prepare slides so as to observe bacterial cultures from yogurt as well as to observe the composition of blood (i.e. red blood cells‚ white blood cells‚ and platelets). Procedure: Exercise 1: Viewing Prepared Slides To begin this lab experiment I
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can be used to observe the active Streptobacillus bacteria in yogurt. Purpose: The purpose of this lab was to display knowledge of the use of a compound microscope with and without an oil immersion lens while observing and identifying various bacterial shapes and arrangements‚ including a self prepared yogurt culture. Procedure: Exercise 1: Viewing Prepared Slides 1. After setting up and cleaning all lenses‚ place the prepared e slide properly on the stage. 2. View the slide under
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Identification of Bacterial Pathogens basic skills in diagnostic bacteriology Dr.T.V.Rao MD Dr.T.V.Rao MD 1 Identification of Microorganisms • For many students and professionals the most pressing topic in microbiology is how to identify unknown specimens. • Why is this important? • Labs can grow‚ isolate and identify most routinely encountered bacteria within 48 hrs of sampling. • The methods microbiologist use fall into three categories: ♣Phenotypic- morphology (micro and macroscopic)
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blood cells (Large+++) found in the urine. The bacterium identified in this specimen is Pseudomonas aeruginosa. This is confirmed by involving many tests and observation‚ such as Gram stain‚ oxidase test‚ catalase test and the observation of colony morphology on Horse Blood Agar‚ MacConkey agar and Tryptic Soy Agar. Pseudomonas aeruginosa is an opportunistic pathogen‚ known as the third most common pathogen associated with nosocomial infections‚ mainly
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Microbiology Module 02 Homework Assignment Use the information presented in this module along with additional outside research to answer the questions: 1) Compare and contrast prokaryotic and eukaryotic. a) Prokaryotes and eukaryotes are two types of cells that are very different but share some certain properties such as methods of reproduction‚ protein synthesis‚ an organized metabolism‚ response to stimuli‚ and plasma membranes. One significant difference is that prokaryotes are without a cell
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