CONFIDENTIAL AS/OCT2010/MIC455 UNIVERSITI TEKNOLOGI MARA FINAL EXAMINATION COURSE COURSE CODE EXAMINATION TIME GENERAL MICROBIOLOGY MIC455 OCTOBER 2010 3 HOURS INSTRUCTIONS TO CANDIDATES 1. 2. This paper consists of ten (10) questions. Answer ALL questions in the Answer Booklet. Start each answer on a new page. Do not bring any material into the examination room unless permission is given by the invigilator. Please check to make sure that this examination pack consists of: i) ii) the
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Bacteria Prokaryotic & Eukaryotic Cells 1-7.Bacteria cells reproduce ASEXUALLY BY BINARY FISSION. Asexually means the offspring comes from one parent Draw what Binary Fission of a bacteria looks like: 8. Label the parts of this prokaryotic cell. [pic] 9. Name several structures that are found in eukaryotic‚ but NOT prokaryotic cells. 10. What type of cells are the most numerous on Earth? 11. What are the most common type of prokaryotic cells? 12. How old are the earliest
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Bacteria can be distinguished from one another by their morphology (size‚ shape‚ and staining characteristics). In this lab experiment‚ bacterial morphology was examined by observing both stained and unstained organisms. A wet mount is a preparation process where a live specimen in culture fluid is placed on a slide and the organism is free to move about. In the wet mount slides provided via LabPaq software with cheek‚ dental plaque‚ and yeast specimens were observed. The wet mount preparations
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temperature required to destroy many species of bacteria‚ fungi‚ viruses and yeasts is approximately 60C (van Doornmalen‚ 2008). However‚ some bacterial species contain endospores that are known to be thermoduric. When a bacteria is thermoduric‚ it is able to withstand prolonged exposure to temperatures up to an around 100C. Many endospore-forming bacteria are well known for the possible pathological dangers they hold. Therefore‚ it is vital develop methods by which these bacteria are effectively and
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this experiment was to identify a specific unknown bacterium that was isolated from a multitude of bacteria in a sample of contaminated dirt by running it through a series of identification techniques. Some of these techniques were simple staining‚ gram staining‚ and isolation techniques. The hypothesis of this experiment was that the bacterium is of the bacillus family due to the rod shape. Due to multiple complications with the PCR‚ a new hypothesis couldn’t be formulated. Methods: In this experiment
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Isolation and identification of an unknown bacterium Pillay‚ Esmerelda (209504371) School of Biochemistry‚ Genetics and Microbiology Department of Microbiology University of Kwa-zulu Natal 25 October 2010 ABSTRACT Different types of bacteria in various forms are found all around us‚ and it is a microbiologist’s job to be able to identify these bacteria. Using various staining techniques and physiological tests‚ an isolated bacterium can be identified. In this experiment‚ a single bacterial colony
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Virtual Lab: Classifying Using Biotechnology Worksheet NOTE: As you read the information in the Microbiology Handbook‚ there may be some terms you are not familiar with – such as 16s ribosomal RNA and Polymerase Chain Reaction. Refer to your text to read background material explaining any terms or processes with which you are not familiar. Record the results of your investigations of each unknown in Table 1 by completing the following steps: 1) Apply the stain to your first
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Bacterial Smears Are Fixed before Staining to? Answer It is important to heat fix the bacterial smear before staining so as to‚ kill the bacteria‚ firmly adhere the smear on to the microscopic slide to prevent washing off during staining‚ and to allow the sample to readily take up the stain. Reference: www2.hendrix.edu What is the purpose of heat- fixing the smear? It helps the cells adhere to the slide so that they can be stained. The purpose of heat fixing is to kill the organisms without
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Study guide for Microbiology Chapter 2 Name________________ 1. List and describe the sequence steps routinely used to identify bacteria. These are referred to as the “five I’s” in your text. 2. Define / describe each of the following as they apply to microbiology: a. Culture f. pure culture b. Inoculum g. contaminated culture c. Inoculation h. mixed culture d. Colony 3. Microbiologists employee a number of approached to acquiring a pure culture
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cytochrome oxidase test on both organisms; again‚ organism A was oxidase positive while organism B was oxidase negative. Then I decided I would run the biochemical tests on organism B. I observed that organism B was non-motile and did not produce endospores. With these results‚ I narrowed down the possible species to Enterococcus faecalis‚ Enterococcus faecium‚ Lactococcus lactis‚ Leuconostoc mesenteroides‚ Pediococcus parvulus‚ and Streptococcus mitis. After obtaining some background information
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