closely related to eukaryotes.| ____ 4. Bacteria that cause disease are called a.|viruses.| b.|pathogens.| c.|endospores.| d.|antibiotics.| ____ 5. Where are you likely to find a photoautotroph? a.|in your refrigerator| b.|in the darkness of the ocean| c.|in your digestive system| d.|near the surfaces of lakes‚ streams‚ and oceans| ____ 6. A method called Gram staining is used to tell a.|what shape a prokaryote has.| b.|how a prokaryote obtains energy.| c.|what kind of cell wall
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and 100°C for 2‚ 5‚ 30 and 90 minutes. As the same in experiment one‚ these cultures were then left for a week to incubate‚ then upon completion they were observed for distinguishable changes‚ such as the turbidity‚ clarity and the formation of endospores to show the bacteria’s survivability at particular temperatures. Information was then collated from other work benches to show the results from all five species. Results Table 1. Table of results showing the effect temperature has on the growth
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Introduction: The purpose of this lab is to use staining techniques and biochemical testing to identify an unknown bacteria using Bergey’s manual. Bergey’s manual of Systematic Bacteriology is a dichotomous key primarily used to identify a bacterial species. Biochemical tests are used to differentiate different species of bacteria. These tests are effective in determining the characteristics of the microbe being tested. Such characteristics include citrate utilization‚ gelatin hydrolysis‚ nitrate
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observations. It was hard to tell what I was looking at. There were a number of amoeba shaped cells of varying sizes. There were five darker areas circular in nature. There were also three very large‚ odd shaped areas with distinct edges. Part 4: Direct Staining: Record your observations for each sample. Slide One: There were two distinct clusters that were easily noted. All the cells were cocci. Some of the cells were huge while others were practically nonexistent. Slide Two: There were layers of cells
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inhabit the intestine of humans. It is normally a Gram-negative‚ rod-shaped bacterium. It is about to survive in physiologically high temperatures that allows up to excess of 50oC. (Daniel C‚ 2008). However it is not able to resist temperature like endospore. Therefore‚ the amount of bacteria would decrease as the heating persists. Germicides are disinfectants or chemicals used to kill germs which are mainly pathogenic microorganisms present in the environment. (Farlex‚ 2012) Germicides are formulated
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John Franklin Farrar Department of Biology and Microbiology and Biology Address BOX 22750 BOWEN HALL‚ RALEIGH‚ NC‚ 27607 Abstract: Isolation and characterization of microorganisms is a practice that aids in Increasing ones knowledge of a laboratory setting and it helps improve on Using sterile technique. Isolates of soil microbes can be categorized and Characterized based on a number of criteria ranging from gram-staining Which is done for this project to enumeration which
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most important tests done was gram staining because it helped to limit the possible bacterium that it could have been. To be sure which bacterium was worked with we referred to the Bergey’s manual and compared the results to the possible bacterium. Results:
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LABORATORY EXERCISE 8-A: Preparation of Bacterial Smear and Simple Staining I. INTRODUCTION Bacterial smears are prepared for the purpose of viewing microorganisms under the microscope. Visualization of microorganisms in the living state is very difficult‚ not just because they are minute‚ but because they are transparent and almost colorless when suspended in an aqueous medium. A bacterial smear is a dried preparation of bacterial cells on a glass slide. Smears may be made from a dry culture
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in the staining methods‚ the cells and their borders were very distinct while the one in the wet mounts were not as distinct. It was a bit challenging to determine the figures in the cheek smear‚ but the bacteria form in the yeast wet mount were easily recognized. The direct staining was the following technique used. The cells were very easy to assess‚ well demarcated and had a very distinctive color. Instead of the background and around the cell in the cheek smear‚ the indirect staining way seemed
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Procedure: After setting up the incubator‚ I aseptically transferred S. epidermidis and L. acidophilus. to generate liquid broth cultures. After waiting for 24-48 to observe growth‚ I recorded my observations. Then‚ I prepared wet mount slides and direct staining slides of both S. epidermidis and L. acidophilus. to observe them microscopically using oil immersion lens. When I was done‚ I stored them in the refrigerator for future use. Observations/ Data Tables: Bacteria 40x 100x L. acidophilus (wet mount)
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