Microbiology 197 Prepared Bacteria Gram Stains (F12) Materials required: * Microscope; clean and properly set up * Immersion oil * Lens paper * Lens cleaning fluid * Microscope drawing forms * Specimens: 1. Bacillus subtilis 2. Staphylococcus aureus. 3. Escherichia coli Procedure: 1. Observe each of slides listed in “Specimens” above. 2. Make your observations using oil immersion (1000X). 3. Using a drawing form draw the organisms
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Chapter 1 Humans and the Microbial World A Glimpse of History Science of microbiology born in 1674 Antony van Leeuwenhoek (1632–1723) • Made simple magnifying glass • Studied lake water • Observed ‘animalcules’ Robert Hooke • Also credited with discovery • Described ‘microscopical mushroom’ (common bread mold) in 1665 1 Importance of Microorganisms Microorganisms are foundation for all life on earth Have existed for ~3.5 billion years Plants‚ animals‚ modern
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MICROBIOLOGY SECTION The microbiology section aims at studying yu ailments and diseases from the isolation of suspected causative organisms. The processes involved in the isolation of these organisms include; culturing‚ staining‚ microscopy and sensitivity tests. Samples collected for examination include; stool‚ blood‚ sputum‚ urine‚ vaginal swab‚ wound swab and wound biopsy. Bacterial Culturing and sensitivity Cultures are carried out to isolate suspected organisms from a sample. There are different
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Unknown Lab Report Microbiology Unknown A Sonia Kabra November 26‚ 2014 Introduction There are numerous reasons for identifying unknown bacteria. Some of these organisms have distinct qualities that set them apart from one another‚ such as the exposure to certain environments. Through out the semester in the laboratory‚ we are able to encounter some of the few microorganisms that we as humans have come into contact with. With the knowledge gained from the sessions in the laboratory‚ we can now
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cause eye infections in men and women which can lead to blindness. It can also be passed from mom to baby during birth in the form of eye infections and pneumonia. HOW WAS IT DISCOVERED? c. Trachamotis was first discovered in 1907 by Giemsa staining by Halberstaedter and von Prowazek who took scrapings from individuals infected with trachoma and inoculated orangutans eyes with the samples. This demonstrated how the disease could be spread between infected individuals. In 1929‚ there was an
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research paper of the effects of tetanus upon the human body; Be careful where you step and get vaccinated! Works Cited: 26. Wells‚ C. L.‚ Wilkins‚ T. D. (1996). "Clostridia: Sporeforming Anaerobic Bacilli". In Baron‚ S.; et al. Baron’s Medical Microbiology. Univ of Texas Medical Branch. ISBN 0-9631172-1-1. 8. "Tetanus" (PDF). CDC Pink Book. Retrieved 2007-01-26. 1 . https://en.wikipedia.org/wiki/Tetnus 2. https://en.wikipedia.org/wiki/Tetanus 3. https://en.wikipedia.org/wiki/Tetnis 4. https://en
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become directly stained. In direct staining‚ the organisms must be fixed by a process such as heat. Fixing the slide prevents the organism form washing off the slide before visualization. This is accomplished by passing a smear of the bacteria through flame. The heat sets the proteins of the organism thus causing the bacteria to attach to the slide. The organism can become damaged from the setting process and the use of heat prior to staining. In indirect staining‚ the negatively charged colored portion
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appearance was observed and recorded. There was growth that appeared pinkish in color. The first test we did was basic stain using a heat fixed emulsion which kills the bacteria and allows them to adhere to the slide and thickens their protein for better staining. I then covered the heat fixed emulsion with crystal violet for one minute. The stain was then rinsed with distilled water. The stain was then pat dry with bibulous paper then observed under oil immersion to determine morphology. Upon completion
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Chapter 17 Louis Pasteur’s experiment illustrated that: microbes will not grow in a nutrient broth that has been sterilized unless air is allowed to enter the vessel through an opening. Living organisms can spontaneously generate from nonliving matter. True or False? False The scientists usually given credit for disproving the theory of spontaneous generation of bacteria are: Tyndall & Pasteur Miller and Urey’s experiments attempting to recreate the prebiotic environment produced a rich mixture
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sputum sample and will be testing for one of the following genera: Bacillus‚ Escherichia and Mycoplasma. In order to determine which one of the three may be the source of the infection we have to recognize the characteristics of each one and the staining protocol needed to identify them. All three of the above mentioned genera are considered to be members of the Prokaryotic cell family which includes bacteria. They have certain identifiable features that distinguishes them from Eukaryotes such as:
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