Methyl Red and Voges-Proskauer Test The Methyl Red test shows which bacteria are creating stable acids through mixed acid fermentation of glucose. This helps to identify enteric bacteria by examining how they metabolise glucose. Every enteric bacteria first produces pyruvic acid from metabolism of glucose. A methyl red positive enteric bacteria‚ uses the mixed acid pathway when breaking down pyruvic acid to different acids‚ like lactic‚ acetic‚ and formic acids. The Voges-Proskauer test determines
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Performed: September 9 ‚2011 SPECTROPHOTOMETRIC DETERMINATION OF THE ACID DISSOCIATION CONSTANT OF METHYL RED M.C. Caligagan ‚M.N.Q. Tolentino and M.Q. Clores Institute of Chemistry‚ College of Science University of the Philippines‚ Diliman‚ Quezon City‚ Philippines Received :September 21‚2011 ABSTRACT This experiment aims to determine the acid dissociation constant value of Methyl Red by means of spectrophotometry. Ten samples were spectrophotometrically analyzed under a UV-Vis spectrophotometer
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Abstract:The Wittig Reaction is a nucleophilic addition in which an alkene is formed as a product. Both the E and Z isomers of the alkene result. Substituents on the aromatic aldehyde affect the E/Z ratio of products that form. In this experiment‚ a nitro group was used as the substituent in the ortho‚ meta and para positions‚ with benzaldehyde as the control. Each of the four aldehydes reacted with (carbethoxymethylene) triphenylphosphorane to produce ethyl cinnamate‚ ethyl-3-(2-nitrophenyl)acrylate
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Simple Distillation of Ethyl Acetate contaminated with Methyl Violet Operational OrganicChemistry A Problem Solving Approach to the Laboratory Course‚ 4th edition‚ by John Lehman‚ Experiment 4‚ OP-30 pg 710-719 I. Introduction The students will use the distillation method to extract pure Ethyl acetate by using the distillation equipment. The distillation is the process of vaporizing a liquid mixture in one vessel and condensing the vapors into the receiver. II. Experimental procedures. 1
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present in a sample. Primarily‚ the aim of Experiment 1 was to measure the absorption spectrum of a particular coloured substance (in this case Bromophenol Blue and Methyl Orange) at varying wavelengths of light. The goal of this experiment was to measure the given amounts of a given chemical substance that was present in a given sample of Methyl Orange and in blood. We measured the optical density at wavelengths 400 to 700 in intervals of 20 mu. For Experiment 2‚ the process of the experiment focuses
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5. ENERGY BALANCE Basis:- 1 Hr. Operation ENERGY BALANCES ACROSS THE FEED PREHEATING EQUIPMENT:The heating of cold 2-butanol feed is to be completed in three stages in order to avoid large heat losses. The cold feed is first preheated to its boiling point using steam as heating medium and then vaporized in a thermosyphon reboiler utilizing the heat contained in the reaction products. The vapor‚ which has entrained liquid is removed from the knock out drum‚ and then heated to reaction temperature
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manufacturer to determine if sodium benzoate‚ a common food preservative‚ undergoes significant chemical changes at the pH level encountered in the acidic environment of the stomach. Experimental Method Sodium benzoate was prepared by addition of an equimolar amount of aqueous sodium hydroxide (3 M‚ ~ 20 ml) to a benzoic acid (1.256 g‚ 10.3 mmol) /water mixture (10 ml). Benzoic acid is insoluble in water. The hydroxide base rapidly deprotonates the acid‚ producing the benzoate anion which is readily soluble
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M. Ashrafuzzaman et al / Research Journal of Biology (2012)‚ Vol. 02‚ Issue 03‚ pp. 98-103 ISSN 2049-1727 Research Paper Comparative Study on Regeneration Potentiality of Aromatic Indica Rice (Oryza sativa L.) of Bangaldesh Golam Gaus Mohiuddin Chowdhury1‚ M. Ashrafuzzaman*1‚ F. Raihan2 and Shamsul H. Prodhan1 1 Department of Genetic Engineering and Biotechmology‚ School of Life Sciences‚ Shahjalal University of Science and Technology‚ Sylhet-3114‚ Bangladesh 2 Department of Forestry
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Global And China Methyl Isopropyl Ketone Industry 2014 Market Research Report The report firstly introduced Methyl Isopropyl Ketone basic information included Methyl Isopropyl Ketone definition classification application industry chain structure industry overview; international market analysis‚ China domestic market analysis‚ Macroeconomic environment and economic situation analysis‚ Methyl Isopropyl Ketone industry policy and plan‚ Methyl Isopropyl Ketone product specification‚ manufacturing
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Preparation of 4-bromoaniline Introduction Aromatic compounds tend to undergo electrophilic aromatic substitutions rather than addition reactions. Substitution of a new group for a hydrogen atom takes place via a resonance-stabilized carbocation. As the benzene ring is quite electron-rich‚ it almost always behaves as a nucleophile in a reaction which means the substitution on benzene occurs by the addition of an electrophile. Substituted benzenes tend to react at predictable positions. Alkyl groups
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