Determining the Osmolarity of a Potato Mishal Hasan Abstract Students in Biology find it difficult to understand the concept of tonicity and osmolarity in a real time situation. In this investigation‚ several concentrations of sucrose were used to determine the osmolarity of a potato. It was found that the concentration of sucrose was close to 3.6 in the potato used showing that it was isotonic. Anything above caused the potato to gain weight showing that it was a hypotonic solution and anything
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an experiment from which I should be able to draw a firm conclusion that will either prove or disprove any predictions I make. This essay aims to assess and investigate the effect of various solution concentrations on the activity of osmosis in plant tissue. Background scientific theory: Plants exchange gases (CO2 and O2) in maintaining vital respiratory processes and in carrying out photosynthesis; they absorb certain minerals and sugars so to use as a source of energy and eradicate wastes in
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Unit 4 Lab – Effects of Osmosis on Living Tissue Assignment Total: 35 Points Course Weight: 6 Pre-Lab Preparation - Review the following resources to complete this assignment: Textbook: Chapter 5‚ pg. 83-86 Mastering Biology / Study Area / Chapter 05 – The Working Cell / Student Home Key Concepts Video: Plasmolysis Video: Turgid Elodea Activity: Membrane Structure Activity: Diffusion Activity: Osmosis and Water Balance in Cells Bioflix: Membrane Transport Additional Resources Blast Animation: Diffusion
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Experiment: Turgor in Potato Tissue 1. We labelled three test tubes A‚ B‚ C‚ D and added initials of people in our group. 2. Used a syringe or graduated pipette to put 20 cm3water in tube A and 10 cm3 in tube B. 3. Put 20 cm3 of 17% solution in tube C and 10 cm3 in tube B; put 20 cm3 pure sucrose solutions in tube D. 4. Working on the dissecting board I cut the ends off large potato. I was careful with the knife and cut it onto the dissecting board away from myself. 5. Used a cork
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Osmosis in Potato Tissue Experiment Background Information Osmosis can be defined as the movement of water across a semi-permeable membrane from a region of high water concentration to a region of low water concentration. The semi-permeable membrane allows small particles through it but does not allow large particles such as sodium chloride. Osmosis will continue until a state of equilibrium is reached i.e. there is no area with a higher or lower concentration than another area
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Osmotic concentration‚ formerly known as osmolarity‚[1] is the measure of solute concentration‚ defined as the number of osmoles (Osm) of solute per litre (L) of solution (osmol/L or Osm/L). The osmolarity of a solution is usually expressed as Osm/L (pronounced "osmolar")‚ in the same way that the molarity of a solution is expressed as "M" (pronounced "molar"). Whereas molarity measures the number of moles of solute per unit volume of solution‚ osmolarity measures the number of osmoles of solute
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concentrations of 0.0 mol dm3‚ 0.2 mol dm3‚ 0.4 mol dm3‚ 0.6 mol dm3 and 0.8 mol dm3 affect the osmolarity point of potato cells‚ where it is investigated how osmosis changes the weight of the potato cells‚ measured by the percentage change between the initial and final mass of the potato? Independent Variable: Increasing sucrose concentrations Dependant Variable: Mean percentage change in mass of potato cells All cells have a protective barrier‚ one which selectively allows substances to enter
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Estimating Osmolarity by change in weight Abstract Water flows in and out of cells in an attempt to attain a state of equilibrium. The concentration of solutes to solvent in the cells environment is the cause of the water flow. Plant and animal cells can be negatively affected or positively affected due to the concentration balance in their environment. Potato cells were used to see the affects of sucrose in different concentrations. In some
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Based on the result‚ what is known now is when osmolarity is low‚ the hemolysis is high; while when osmolarity is high‚ the hemolysis is low. This supports the hypothesis if the erythrocytes were placed into a high osmotic solution‚ then the erythrocytes would have a lower hemolysis percentage. As shown in graph 1‚ when the erythrocytes were placed into the tube containing 250 to 390 osmolarity of solution‚ the erythrocytes results in below 26 hemolysis percentage. When the erythrocytes were placed
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Osmolarity TITTLE: practical of estimation of osmolarity in tissues by bathing samples in hypertonic and hypotonic solutions. INTRODUCTION: Osmolarity is the osmolar concentration of plasma and is proportional to the number of particles per liter of solutions shown as (mmol/l). It is derived from the measures Na+ and K+‚ urea and glucose concentrations. Since the volume of solution changes with the amount of solute added also it change in temperature and pressure‚ osmolarity we can say it’s
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