Lab Report 1708 Mrs. Himler Ryan Nelson 9/20/2024. Lab Report INTRODUCTION: During this lab‚ the group used 3 food thickeners. These thickeners are used to thicken‚ stabilize‚ and prevent separation for our daily products such as ranch‚ ice cream‚ and other dairy products. The group used modified food starch‚ corn starch‚ and guar gum. They all are different from each other‚ each in a unique way. Firstly‚ guar gum is like pectin‚ a soluble fiber and polysaccharide. Furthermore‚ the body cannot digest
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Purpose: The purpose of this lab was to observe and understand the effects of changes in temperature‚ pH‚ enzyme concentration‚ and substrate concentration on the reaction rate of an enzyme-catalyzed reaction. Another purpose of the lab was to explain how environmental factors affect the rate of enzyme-catalyzed reactions. Hypothesis: I believe that if there is an increase in enzyme concentration‚ an increase in temperature‚ or an increase in pH‚ then the intensity of the reaction will
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shows RFU’s (left) and total protein in ug (right) for each wash and elution. Once the rGFP had been purified in the Ni2+-agarose column‚ a breaking buffer (10mM Tris‚ pH 8.0; 150mM NaCl) was used to create the washes by pipetting the buffer in 0.5ml increments and collecting each in separate tubes labeling them W1-W6. Then‚ an elution buffer (10mM Tris‚ pH 8.0; 150mM NaCl; 300mM imidazole) was collected in increments of 0.5ml and labeled E1-E6. We could measure the amount of RFU’s each sample had using
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Abstract A buffer is a solution that resists changes in pH when H+‚ OH-‚ or H20 is added. By using standard lab equipment‚ a lab pro diagnostic tool‚ and acidic and basic solutions‚ the pH can be found. By recording the pH while adding a base or an acid gradually to a buffer solution you can find the capacity of each buffer to resist drastic changes in pH. The best buffers will keep a solution from becoming either too acidic or basic with the addition of a strong base or acid. Introduction The
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reaction in which catechol‚ then catechol becomes the product called benzoquinone‚ which is a reddish-brown color‚ which make it easier to determine the quantity that has been formed. (Dickey). Moreover‚ the enzyme activity is persuaded by ph‚ plus temperature. Change in ph will have effect on hydrogen bonding that can modify the form of protein. Increase in the temperature will spread the reactions rate; nevertheless‚ if the temperature is unreasonably high the enzymes will denatures. For this experiment
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Lab Report: pH Name: _________________________________________________________ Materials Needed You will need the following materials for this lab. • • • • • • • • • • • • • Red cabbage Coffee filter or paper towel Container for water (at least 250 ml or 1 / 2 pint) Three transparent cups (about 100 ml or 3 ounces) or other similar containers Hot water (e.g.‚ from a faucet‚ heated in a microwave oven‚ etc.) Thermometer Vinegar Baking soda Safety goggles Tongs or fork Eyedropper or drinking
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dark reactions affect the rate of photosynthesis in chloroplasts. We will also be using a reference solution made of water‚ phosphate buffer‚ and active chloroplasts. The purpose of this solution will be used to set the transmittance level for the experiment. The control solution‚ which is different than the reference solution‚ is comprised of water‚ phosphate buffer‚ and DPIP. It will be
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[Name] [Teacher] [Course] [Date] Catalase Lab Report An enzyme is something that helps to speed up a chemical reaction. The enzyme changes from reaction to reaction‚ but it always has the same impact. However‚ certain variables may cause the enzyme to have a more or less significant impact on the speed of each reaction. One of these variables that changes the effectiveness of an enzyme is temperature. There is an optimal functioning temperature for each enzyme in each reaction‚ depending on
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University of Texas at Tyler Lab 3C: Purification of L-Lactate Dehydrogenase By Affinity Chromatography on Cibacron-Blue Sepharose David Alexander 10-15-2014 Dr. Black Chem 4135.001 Abstract: Like the previous experiments‚ the ultimate goal of this lab was to purify the enzyme sample. However‚ this is the last lab for purification and high level techniques of purification were employed to achieve this. Dialysis was used first‚ lowering the small-molecule concentration within the sample. Finally
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In the first part of the lab‚ we prepared a salivary amylase solution and aggregated acetic acid to detect the presence of mucin. Then‚ 4 test tubes were made of the following: Tube 1 = 3 ml starch + water + 37 degrees water bath; Tube 2 = 3 ml starch + saliva in water bath; Tube
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