between the amount of substrate in the assay solution and the rate of the reaction when the enzyme and buffer in the assay are held constant were experimented. We analyzed the change in absorbencies over time for varying substrate concentrations. There were four experimental assays which contained 1% enzyme solution‚ substrate solution of 0%‚ 1%‚ 2%‚ and 3% concentrations‚ guaiacol‚ and pH 7 buffer. At 2% concentration there was a greater enzymatic activity and at 3% concentration enzymatic activity
Premium Enzyme Chemical reaction Chemistry
and O (Campbell Reese‚ 2008). The environment plays an important role in the reaction that enzymes have. In this experiment‚ enzymes were exposed to changes in temperature‚ pH‚ and concentration. Introduction In this experiment‚ the environment of the enzyme was altered‚ by increasing or decreasing the temperature‚ pH and concentration. The purpose was to observe if and how the enzyme would react to such changes. This experiment tested whether heating or cooling a catalase would increase
Premium PH Enzyme
Purpose Understanding how catalase activity is affected by pH is the purpose of this experiment. Introduction Enzymes play an important role in daily life because of the chemical reactions. Almost chemical reactions require the presence of enzymes to promote the metabolic process. They are known as the incredibly efficient and highly specific biological catalysts. Most enzymes are protein with the ability to enhance the rate of reaction between molecules. To catalyze a reaction‚ the enzymes
Premium Enzyme Metabolism Catalysis
find the pKa of an unknown‚ pH meters are used during titrations to measure the potential difference in a solution by measure the difference of hydrogen-ion activity in a solution and reporting the pH. The pH is then plotted against the volume of solution added which provides the titration curve of a substance. From the titration curve information can be gathered about how many protons were dissociated and the equivalence point. pH=pKa+log([H]/[OH] ) pKa=pH-log([H]/[OH] )
Premium Chemistry Acid Acid dissociation constant
An Experiment to Test the Effect of Temperature on the Rate of Photosynthesis in Green Algae Background Photosynthesis is an amazing process where plants are able to create their own food as well as oxygen using sunlight‚ carbon dioxide (co2)‚ and water. The part of the plant responsible for photosynthesis is the enzymes in the chlorophyll (structures that carry out photosynthesis located in leaves). Photosynthesis is the process of creating glucose. Water + Carbon Dioxide + Sunlight = Glucose
Premium Chemical reaction Temperature Light
Abstract This experiment investigated the kinetics of the enzyme glycogen phosphorylase b which is important to metabolism. AMP is an allosteric activator of the enzyme because it converts glycogen phosphorylase b from its T state to the R state which is the active form. Caffeine is an inhibitor because it binds the nucleoside inhibitor site. When it binds this site‚ it stabilizes the inactive T state and blocks the catalytic site which needs to be open for enzyme activity to occur. The glycogen
Premium Enzyme Metabolism Chemical reaction
Vicente Viloria CHM 130 LL Section 22258 Lab 12: Introduction to pH‚ Household Products and Buffers 12/9/14 Introduction In this experiment the students will be determining the pH of household products along with other solutions using several different indicators as well as a pH meter. The experiment also has the student determining what the buffer solution is in an aqueous solution. The student will also be testing the pH of milk of magnesia and will see how it affects the stomach acid. Lastly
Premium PH Acid PH indicator
This page intentionally left blank Copyright © 2008‚ New Age International (P) Ltd.‚ Publishers Published by New Age International (P) Ltd.‚ Publishers All rights reserved. No part of this ebook may be reproduced in any form‚ by photostat‚ microfilm‚ xerography‚ or any other means‚ or incorporated into any information retrieval system‚ electronic or mechanical‚ without the written permission of the publisher. All inquiries should be emailed to rights@newagepublishers.com ISBN (13) : 978-81-224-2652-6
Premium PH Water PH indicator
Table 1: Data Collection Table – Contains all of the primary data directly obtained from the lab. Indicator | Initial volume of NaOH in burette (ml) ±0.05 | Final Volume of NaOH in burette (ml) ±0.05 | Final – initial Burette Reading (Volume of NaOH used) (ml) ±0.1 | Qualitative Observations | Phenolphthalein | 0.00 | 0.90 | 0.9 | At first when the base was being dropped into the vinegar there wasn’t a color change‚ however when the solutions came close to full titration‚ the solution
Premium PH indicator Sodium hydroxide Titration
VIMAL SIEWNARINE - #52844 JASON MATHURA - #60927 FIDEL MENDOZA- #56834 BRAD NANDO- # VIMAL BALAY - #52555 CRISTINA LUTCHMAN -#52516 LAB #1‚ #2‚ #3‚ #4 CHEM 2006 -ANALYTICAL INSTRUMENTATION LECTURER – MRS. TRICIA JONES LAB 1 TITLE: Organic Compound Identification Using Infrared Spectroscopy Aim: To identify the functional groups in organic compounds using infrared spectra. APPARATUS AND REAGENTS: Nicolet 380FTIR‚ HATR Accessory
Premium PH Concentration Chemistry