The Kirby-Bauer Antimicrobial Susceptibility Test Read section II of chapter 26 in your lecture textbook for background on chemical antimicrobial control (for background)—pages 762-763. Introduction Today you will perform a frequently used procedure called the Kirby-Bauer Antimicrobial Susceptibility test (disc diffusion technique). Each group will inoculate his/her own plate of Mueller-Hinton agar with an assigned culture. To that inoculated plate‚ you will then aseptically add sterile
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QUEENSLAND ACADEMIES FOR SCIENCE‚ MATHEMATICS AND TECHNOLOGY International Baccalaureate Diploma Program Extended Essay in Biology Is Tea-tree Oil effective in controlling and minimising the growth of the Escherichia coli Bacteria? A study of the effectiveness of a Natural Antiseptic Candidate Name: Lois Adrienne Dianne Soriano Dulay Candidate Session Number: 003072-032 Word Count: 3997 Supervisor: Ms Kate Barry Date of Submission: 25th July 2011 Acknowledgments Firstly I would
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PREVALENCE OF ESCHERICHIA COLI ON PHILIPPINE PESO BILL WITHIN THE VICINITY OF ILOILO CITY 2012-2013 A Research Paper Presented to The Faculty College of Medical Laboratory Science Department Central Philippine University Iloilo City In Partial Fulfillment of the Requirement for the Research 1 JUSTINE GRACE G. JIMENEA ISAAC JAMES T. LEDESMA MAJA HANNAH LOU L. LOJA ROBERTO R. MANALO JR. JIRAH L. PEDROSA MARCH 2013 CHAPTER 1 INTRODUCTION Background of the Study A classic
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INFECTION CONTROL Pathogen- Infectious agent Colonization- If microorganism is present or invades host‚ grows and/or multiplies but does not cause infection. Communicable disease- if infectious disease can be transmitted from one person to another. -Symptomatic-if pathogens multiply and cause clinical signs/symptoms. -Asymptomatic- if no s/s are present *CHAIN OF INFECTION: 1. An infectious agent or pathogen 2. A reservoir or source for pathogen growth 3. A portal of exit from the
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2.1 Sanitation Methods There are Four Methods that conducted on the laboratories in order to detect the presence of microorganisms. There are Rodac Method‚ Swab Method‚ Rinse Method‚ and lastly Open Dish Method and it will be discussed in detail below. 2.1.1 Rodac Method The purpose of this Standard Operating Procedure is to describe a program that will adequately measure the efficacy of disinfection of Rooms and equipment in each laboratory‚ RODAC plates can detect the presence or absence of
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biochemical tests will be conducted on this unknown bacterium to identify it out of 6 possible bacteria; Escherichia coli(E. coli)‚ Enterobacter aerogenes (E.aerogenes)‚ Klebsiella pneumoniae (K.pneumoniae)‚ Proteus mirabilis(P.miranilis)‚ Pseudomonas aeruginosa(P.aeruginosa)‚ and Salmonella typhimurium (S.typhimurium). The biochemical tests utilized were; Triple Sugar Iron Agar (TSIA) test‚ Sulfur Indole Motility (SIM) test‚ Methyl red test and Voges-Proskauer (MR-VP) test‚ Citrate test‚ Urea test
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Biotechnol. Bioprocess Eng. 2002‚ 7: 57-66 Hydrolysis of Oils by Using Immobilized Lipase Enzyme: A Review V Ramachandra Murty*‚ Jayadev Bhat‚ and P. K. A. Muniswaran . Department of Chemical Engineering‚ Manipal Institute of Technology‚ A Constituent Institution of Manipal Academy of Higher Education (Deemed University)‚ Manipal-576119‚ India Abstract This review focuses on the use of immobilized lipase technology for the hydrolysis of oils. The importance of lipase catalyzed fat splitting
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Review all chapters in your exercise manual beginning with the introduction INTRODUCTION: (Covered 9.4.14 II Week 1) Biosafety levels1: basic level of containment. Hand washing or wearing gloves 2: Appropriate for working with human body fluids. Autoclave‚ sharps containers‚ lab coats 3: appropriate for working with pathogens that can be transmitted via respiratory route. Self-closing‚ double doors and sealed windows 4: Highest level. Aerosol pathogens; pathogens with no vaccine/treatment. Separate
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locates generally inside the specialized nodular structures of root cells. The bacterial genera such as Agrobacterium‚ Arthrobacter‚ Azotobacter‚ Azospirillum‚ ‚ Bacillus‚Burkholderia‚Caulobacter‚Chromobacterium‚Erwinia‚Flavobacterium‚Micrococcous‚Pseudomonas and Serrata belongs to ePGPR.The iPGPR belongs to the family of Rhizobiaceae includes Allorhizobium‚Bradyrhizobium‚Mesorhizobium and Rhizobium.Plant growth promoting rhizobacteria promote plant growth either directly or indirectly. In direct method
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et al in 2008. They checked the salt stress tolerance in both and proved kabuli cultivar were more resistant. The study on bacterial interactions with legumes by Deepak K.M. et al 2011 using Pseudomonas isolates resulted in stunted development of root and shoot due to production of IAA. Pseudomonas coinoculation with Mesorhizobium species resulted in enhanced growth and nodulation in chick pea. Mayuri S.R. et al 2011 also reported the additive Lead detoxification effect in legume when in combination
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