contain protein and which do not? How do you know? Egg white and pepsin contained protein as indicated by the appearance of a purple color in solution‚ whereas sugar and distilled water did not‚ as indicated by no color change from the blue biuret reagent. Questions A. Which of the test tubes are the positive and negative controls? The experiment illustrated that: The Positive controls were Test tube #1 Egg white and #2 Pepsin. The Negative controls were Test tube #4 Distilled water and #3
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–NH2 to give a purple colour. There are three samples in the experiment: sample A‚ Sample B and Sample C. The sample A contains 2 distilled water and ninhydrin reagent. There are egg white and ninhydrin reagent in sample B. Succinylated egg white and ninhydrin reagent were in sample C. Because distilled water cannot react with ninhydrin reagent‚ the sample A was as a blank in the experiment. We measured the absorbance of the egg solution at 570nm. In sample B‚ the native egg white was added. The data
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Title: Double Replacement Reactions (Data and Calculations) Objective: Classify the chemical reaction through observation‚ which each reagent produce when mixed with another reagent. After careful observation‚ be able to prove each observation using the net ionic equation. Background: First‚ a double-replacement reaction is when two cations in different compound switch anions‚ AX + BZ → BY. If either compounds are insoluble a precipitate occurs‚ and if there is no precipitate formed there is
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Materials: * Test tubes * Solutions : ( A‚B‚C‚D‚E‚F‚ and G) * Reagents: ( Iodine‚ Biuret‚ Benedict‚ Ethanol) * Spatula * Heating plate * A test tube cleaning brush * Tray * Beaker * Distilled water * Pipette ( comes with reagents since they are in bottles) Method/Procedure: Step #1: poor the solution into four different test tubes Step #2: In the first test tube put a few drops of a reagent called "biuret". If the solution turns purple‚ then the solution contains
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aluminum chloride‚ fuming sulfuric acid‚ acetyl chloride‚ chromic acid‚ iodoform‚ Lucas’ test‚ Anilide formation‚ ester formation‚ hydrolysis‚ hydroxamic acid‚ Benedict’s test‚ Chromic test‚ 2‚4-Dinitrophenylhydrazine‚ Fehling’s solution‚ Schiff’s reagent‚ Tollen’s test‚ bromine water‚ cerric ammonium nitrate‚ ferric chloride‚ esterification‚ sodium bicarbonate‚ silver nitrate‚ Barfoed’s test‚ Borax Test‚ Bial test‚ Osazones‚ Seliwanoff’s test‚ Molisch test‚ Beilstein test‚ hydroxamic test‚ sodium
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TEST | REAGENTS | PROCEDURE | RATIONALE/PRINCIPLE | POSITIVE REACTION | PHENYLHYDRAZINE | phenylhydrazine | | The aldehyde groups of simple carbohydrates will react with phenylhydrazine but instead of yielding typical phenylhydrazones they make what is termed osazones where the OH group immediately adjacent to the keto group is oxidized to a keto group and it too adds phenylhydrazine to form the yellow to pale orange osazones that have definite melting points. So assignment of presumptive identity
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BIOMOLECULES 1.What are macromolecules? Give examples. Macromolecules are large complex molecules that occur in colloidal state in intercellular fluid. They are formed by the polymerization of low molecular weight micromolecules. Polysaccharides‚ proteins‚ and nucleic acids are common examples of macromolecules. 2. Illustrate a glycosidic‚ peptide and a phospho-diester bond. (a) Glycosidic bond is formed normally between carbon atoms‚ 1 and 4‚ of neighbouring monosaccharide units. (b) Peptide
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different substances. Introduction Macromolecules are in all forms of life. These organic compounds are carbohydrates‚ lipids‚ proteins‚ and nucleic acids. These are monomers and they link together into long chains that form polymers. Different reagents can be used to find the presence of these macromolecules. Proteins are made of carbon‚ hydrogen‚ oxygen‚ nitrogen‚ and sometimes sulfur. Proteins are covalently bonded in chains as polymers of amino acids. These bonds are called peptide bonds.
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Course: Principles of Biochemistry I & Biochemistry I Class: BS- Bioinformatics & BS-Biosciences Lab Instructor: Ms. Sadia Jabeen Lab Protocol #
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The limiting reagent it CuCl2 because it is the one that will run out first compared to the .009 moles of Na2Cl3‚ which it the excess reagent because it is a higher amount of moles compared to the .007 moles of the CuCl2. The amount of excess reagent in grams that should remain in solution if the theoretical yield of CuCO3 is produced is: 1 mole of CuCl2 (63.55) +(2*35.45) =134
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