of Methods: To accomplish this objective‚ each pair of lab partners received an E. Coli lysate sample which we had to thaw by warming it with our hands and centrifuge it. We extracted a total of 12 samples with known concentrations of bovine serum albumin and 10 samples of different amounts of lysate put into them. Using the different assays explained later in this report‚ we could plot a standard curve with the different amount of BSA concentrations on the x-axis as the independent variable and
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nutrient Metabolism Dietary Chromium Supplementation with or without Somatotropin Treatment Alters Serum Hormones and Metabolites in Growing Pigs without Affecting Growth Performance1«2 CHRISTINA M. EVOCK-CLOVER‚3 MARILYN M. POLANSKY‚* RICHARD A. ANDERSON* AND NORMAN C. STEELE Nonruminant Animal Nutrition Laboratory and *Vitamin and Mineral Nutrition Laboratory‚ USDA-Agricultural Research Service‚ Beltsuille‚ MD 20705 increase in insulin internalization in rat muscle cells (Evans and Bowman 1992)
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to detect or chek presence of cancers generaly. these biochemical indicators are abnormal molecules secreted by the tumor celssand they are included surface anitigens‚ cytoplasmic proteins‚ enzymes and hormones. These markers are measured from the serum and are used in screening tests of the population to evaluate presence of cancer. These markers are also important in supporting diagnosis of cancers. And also in addition they are used in monitoring response of the therapies and the detection of recurrence
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CELL TRANSPORT MECHANISMS AND PERMEABILITY Zack Johnson Human Anatomy & Physiology Lab 2101 Instructor: Alan Byboth‚ M.S. 10/4/12 Abstract- The cell is invaluable building block of all biological life on this planet‚ and one of its most important and unique characteristics is its ability to be selectively permeable with its plasma membrane. This outer membrane’s sophisticated mechanisms of transport through its bilayer are vital in maintaining homeostasis in the cell and the entire
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Title: Immunology Aim: To visit a microbiology department at the university hospital and acquire detailed information of various antigen-antibody tests eg. ASO‚ haemotoglutination‚ ELISA etc. Method: To listen to presentation made and thus formulate a concise report on that which was discussed and demonstrated. (Please see BC34D Laboratory Manual for more information) Concise Report/Summery: The term serology refers to both the study of general antigen-antibody reactions in a laboratory
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and antibody outside the body can be used to determine if patient has an infectious or an autoimmune disease. In this particular exercise‚ the sample blood is collected from three different patients. And after‚ separating the red blood cells and serum‚ a serum was used to perform the ELISA test. a. ELISA Test ELISA is an abbreviation for “Enzyme-linked Immunosorbent Assay." An ELISA test uses components of the immune system and chemicals to detect immune responses in the body‚ such as infectious
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This is done by injecting the patient’s serum or stool into the peritoneal cavity of mice. An equal amount of serum or stool from the patient is treated with multivalent antitoxin and injected in other mice. If the antitoxin-treated serum- or stool-injected mice live while those injected with untreated serum or stool die‚ then this is a positive test for botulism and is called the mouse inoculation test. The bacteria can also
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(43 kDa) overexpression was remarkably observed (Fig 2A). In order to further characterize LIC13341‚ polyclonal antibodies were generated in BALB/c mice against r-LIC13341. To check its antigenic property‚ ELISA was performed with the mouse immune serum raised against r-LIC13341. The immune response against r-LIC13341 showed a titre of around 1:40000 (Fig 2B). This suggests that LIC13341 is an antigenic protein and there is a host
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In our experiment‚ we will be testing three different proteins to see if they denature at the same temperature. A protein is a molecule containing amino acids created in ribosomes that have many functions‚ including being an enzyme and transporter‚ structuring‚ translating genetic information‚ and serving as a channel for charged molecules to pass through the cell membrane. As a molecule‚ a protein is shaped like a coil (Food Science Basics 2014). Denaturation is when a protein’s shape changes due
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Blood Analysis: Computer Simulation Introduction Blood is a combination of plasma (watery liquid) and red and white blood cells as well as platelets. It is a specialized bodily fluid that supplies essential substances and nutrients‚ such as sugar‚ oxygen‚ and hormones to our cells. Blood analysis can determine how functions of the body are working and if they are working properly. The purpose of this lab is to understand how various ailments‚ locations‚ and blood types can affect blood analysis
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