ESTIMATION OF IRON IN IRON ORE-SPECTROPHOTOMETRIC METHOD By: Taylor Villari Experiment conducted on 7/22/13 Components of each test tube examined in the spectrophotometer Trial | Volume of Iron solution (mL) | Micrograms of Iron | Volume of 10% sodium acetate | Volume of 0.1% o-phenanthroline | Volume of water (mL) | 1(blank) | 0.0 | 0.0 | 1.0 mL | 1.0 mL | 8.0 mL | 2 | 1.0 mL | 10 | 1.0 mL | 1.0 mL | 7.0 mL | 3 | 3.0 mL | 30 | 1.0 mL | 1.0 mL | 5.0 mL | 4 | 5.0 mL | 50 | 1.0 mL
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Halobacterium Lab Investigation Mason Pirio 12/12/13 Period 5 Table Group 3 PS 2- Experiment Design PS 3- Conclusion Writing PS 13- Nitrogen Cycle Abstract- In this experiment we tested to see how well halobacterium grows in different levels of salinity. We found out that the higher salinity in the growth medium the better the halobacterium grows. Background Information- Halobacterium is a bacteria that is found in the great salt lake. Halobacterium is an extremeophile which means it thrives
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Abstract: In this Lab we used the chemical DPIP to detect the rate of succinate broken down by the mitochondrial solution. We detected the amount of DPIP in the solution with a spectrophotometer and measuring the absorbance of light at the 600nm range. DPIP is a useful chemical to use in this experiment because it goes from a blue color when oxidized to a colorless liquid (Ogura‚ 281)‚ this is due to the hydrogen ions and electrons released during the transitional step between succinate and fumarate
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Purpose: To find out the densities and to find out the name of the unknown metals. (Based on the extensive and intensive properties) Check up the words mass‚ volume‚ density‚ extensive properties‚ and intensive properties. Where do the units for mass and volume) come from and what do they mean? What is the density of distilled water? What is Archimedes principle? Does temperature affect the density of a solid? Liquid? Gas? Materials: Safety glasses‚ 10‚ 25 or 50 mL graduated cylinders
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Biology‚ Bio 110; October 1‚ 2014 Observing Membrane Structure and Observing Effects of Chemical stress on Membrane Crystal Eve Lopez‚ Dr. Barua Madhabi Keywords: beet root model system‚ spectrophotometer‚ betacyanin‚ cellular membrane‚ phospholipid Abstract The cellular membrane separates and protects the cell acting almost as a wall. Depending on what stressors there are the cellular membrane can become damaged. The objective of this experiment was to examine the struc
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In class‚ we observed this happening using a lamp and a spectrophotometer to measure the amount of light being transmitted through a test tube. Chloroplasts were placed into test tubes along with water‚ DPIP‚ and a buffer to neutralize the pH of the solution‚ and then placed under a lamp with a standard 40-watt light bulb. One test tube was used to calibrate the spectrophotometer so that each transmittance reading would be
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not exist (Lab Manual 3 pg. 1). They help in many different ways that are useful to the body of living organisms. Enzyme are used to speed up chemical reactions (Lab Manual 3 pg. 1). Through this process‚ they are considered very unique because they are not altered or consumed within the reaction (Lab Manual 3 pg. 1). This is why enzymes are considered biological catalysts. They also do not alter the equilibrium of a chemical reaction nor the amount of free energy that is released (Lab Manual 3 pg
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Laboratory Exercise #2 Pre Lab This lab will teach me how to use a spectrophotometer. The use of the spectrophotometer is to measure the concentration of solute. The solute being measured must be colored and is determined based on the adsorption of light photons on a wavelength. The spectrophotometer uses a beam of light that strikes the diffraction grating that basically forms of prism of light. Then only a specific wavelength of light shines through the spectrophotometer and interacts with the
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The spectrophotometer is an essential tool for biologists and chemists in analyzing chemical and biological samples. Gaining familiarity with its operating protocols and understanding what its outputs mean are very important in the development of lab technique for students of cell biology. This experiment will help laboratory students gain experience in using the spectrophotometer. Basic Laws of Light Absorption - For a uniform absorbing medium (solution: solvent and solute molecules that absorb
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catalyzes the substrate molecule into a product. Enzymes have the capability to break or build compounds which keep cell systems functioning. For example‚ our digestive tract has catabolic enzymes which break apart food for storage and reuse. Our lab experiment studied the relationship of benzoquinone production when altering the pH and temperature of the reaction‚ as well as enzyme and substrate concentrations. We used a Spec 20 to evaluate absorbance at 540 nm. Absorbance represents rate of
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