patients and health-care workers in the workplace. Why is it becoming more prevalent in Australian health-care sectors. What is Vancomycin-resistant enterococci (VRE)? Vancomycin-resistant enterococci are enterococcus strains‚ such as Staphylococcus faecalis‚ now called Enterococcus faecalis that are resistant to the antibiotic vancomycin. Enterococci infections are the most common type acquired by hospitalised patients. Vancomycin was the last-line antibiotic for hospital treatment of serious
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Lab Session 8: Selective and Differential Media‚ Unknown ID (Enzyme Based Tests) Oct. 25-31st (To be turned in PRIOR to start of recitation for lab 8) Name: _____ ________ Objective: Analyze microbes from last week. Understand the use of antibiotics on microorganisms. Gain more knowledge about selective media and differential media. Practice use of the catalase test‚ coagulase and the oxidase test. Observe microbial flora of the nose. Significance: Understand the use of Mannitol
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of the two experiments was to determine the fundamental effects that temperature has on the growth and survival of bacteria. During the first experiment five different bacterial broth cultures of Escherichia coli‚ Pseudomonas fluorescens‚ Enterococcus faecalis‚ Bacillus subtilis and Bacillus stearothermophilus were individually incubated at temperatures of 5‚ 25‚ 37‚ 45 and 55°C for one week in an aim to distinguish the effect temperature has on growth and survival of the five different species.
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via the casease. Materials and Methods Bile Esculin Hydrolysis The organisms Lactococcus lactis and Enterococcus faecalis were spot-inoculated on a bile esculin agar plate. The bile esculin agar plate is a both selective and differential medium contains primarily esculin. The plate was then inverted and incubated at 37 oC for 24 hours. Bile salts‚ the selective agent‚ can allow only Enterococcus and group d streptococcus to hydrolyse esculin in the presence of bile salts‚ and inhibit growth of other
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tests indicated that the unknown #4 was Streptococcus pyogenes. All seven tests on the unknown matched S. pyogenes perfectly. The blood agar plate proved the unknown to be β hemolytic‚ meaning the unknown bacteria was capable of complete hemolysis. This test separated the unknown into the β Streptococcus group‚ narrowing the possible bacteria to S. aureus or S. pyogenes. The Catalase test was used to determine if the unknown could break down hydrogen peroxide. The unknown bacteria was unable to break
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3% H2O2 solution‚ swabs. Micrococcus luteus‚ Enterococcus faecalis‚ patient G Procedure: 1) Scrape some cells off from each bateria to the slant and place them on glass slide. 2) Place one or two drops of H2O2. Watch for bubbling as an indication of O2 production. 3) Discard the used slide container. Results: Organisms Bubbles formation Catalase Patient G Bubbles Positive Micrococcus luteus Bubbles/O2 formed Positive Enterococcus faecalis No bubbles/No O2 Negative Observations: When
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Makati Medical Center College of Nursing DRUG STUDY Patient’s Name: M.D.C. Age: 42 y/o Medical Diagnosis: Leptospirosis Attending Physician/s: M.T Allergies: none Allergic Responses: none Prepared by: Shiela Guiquing |DRUG NAME |MECHANISM OF ACTION |RATIONALE FOR THIS PATIENT |ADVERSE DRUG EFFECT |NURSING IMPLICATIONS | |
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Background: Systemic embolism is a common complication of infective endocarditis‚ most frequently involving the central nervous system‚ spleen‚ kidney‚ liver‚ and iliac or mesenteric arteries but embolisation to coronary artery causing sudden cardiac death is infrequently encountered. Case presentation: A case of 45 years old male who had coiling for anterior communicating
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hydrogen will combine with oxygen present in an anaerobic jar to produce water. This system can reproducibly attain oxygen levels in the parts per million range if used correctly. This is the best method for determining the oxygen requirements of unknown organisms. A candle jar is useful for culturing organisms which prefer reduced oxygen levels and increased carbon dioxide levels. The candle jar is not an oxygen free system. This is the best method of culturing a microaerophile. An obligate
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use a systematic battery of tube tests and plates designed to lead to identification of two unknown bacterial species‚ from the combination of all results. A sample of bacteria was used‚ labeled “Sample 4”‚ from which both species was to be obtained‚ one gram positive and one gram negative. Table 1 is a list of the possible bacteria to be identified; the basic ideas and practice of identification of an unknown sample of bacteria are important for a microbiologist to develop. Not only is proper procedural
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