Licania Arborea Leaves Lab Report
Phytochemical march allowed qualitative determining of the main groups of chemical constituents present in Licania arborea leaves. Abundant amount of amino acids, phenolic compounds, triterpenoids and tannins, moderated presence of flavonoids and leucoantocianidines, and the absence of nafto- and antroquinones, cardiotonics and alkaloids was observed. The high content of phenolic compounds happens to be interesting as there is a high probability polar extract from the leaves of this plant have antioxidant activity.
Preliminary experiments with trypan blue and MTT with different concentrations of eleven fractions of L. arborea, showed four fractions exhibit cytotoxic activity (F6, F8, F9 and F10) in Jurkat and CHO-K1 cell lines. Antiproliferative …show more content…
An approximated IC50 of 100 µg/ml was also obtained with both fractions and cell lines. Besides, it was observed that the F8 and F10 fractions reduced the cloning efficiency in both cell lines, depending on the dose. On the other hand, 100 µg/ml of the F8 and F10 fractions did not showed significant difference with positive control, nevertheless, the same concentration of the F8 fraction concentration caused almost total inhibition in the growing of CHO-K1 cells. These results are similar to others reports in K562 lymphoid cell line treated with dichlorometanolic fractions of L. arborea …show more content…
Besides, the F8 fraction showed effect in SCE number and mitosis absence in Jurkat cells treated with 100 µg/ml concentration, related with a lower RCE and reduction in viability obtained with trypan blue in the same concentration. Despite this, antiproliferative effect found in cloning efficiency was independent of the cell cycle as non-significant differences of proliferation kinetic and accumulation function corroborate. A possible explanation could consider the assessment time of the test used. In other hand, short term tests, such as MTT and trypan blue, assess immediate cytotoxic action to mitochondrial and membrane level, respectively; while long term test, such as cloning efficiency, SCE, and accumulation function, assess cell repairing or dead caused by the assessed
Preliminary experiments with trypan blue and MTT with different concentrations of eleven fractions of L. arborea, showed four fractions exhibit cytotoxic activity (F6, F8, F9 and F10) in Jurkat and CHO-K1 cell lines. Antiproliferative …show more content…
An approximated IC50 of 100 µg/ml was also obtained with both fractions and cell lines. Besides, it was observed that the F8 and F10 fractions reduced the cloning efficiency in both cell lines, depending on the dose. On the other hand, 100 µg/ml of the F8 and F10 fractions did not showed significant difference with positive control, nevertheless, the same concentration of the F8 fraction concentration caused almost total inhibition in the growing of CHO-K1 cells. These results are similar to others reports in K562 lymphoid cell line treated with dichlorometanolic fractions of L. arborea …show more content…
Besides, the F8 fraction showed effect in SCE number and mitosis absence in Jurkat cells treated with 100 µg/ml concentration, related with a lower RCE and reduction in viability obtained with trypan blue in the same concentration. Despite this, antiproliferative effect found in cloning efficiency was independent of the cell cycle as non-significant differences of proliferation kinetic and accumulation function corroborate. A possible explanation could consider the assessment time of the test used. In other hand, short term tests, such as MTT and trypan blue, assess immediate cytotoxic action to mitochondrial and membrane level, respectively; while long term test, such as cloning efficiency, SCE, and accumulation function, assess cell repairing or dead caused by the assessed