human body samples by culturing them on the appropriate media using aseptic transfer techniques. Materials • Distilled water • Test tube • 6 Unopened packages of 1 sterile cotton swab • 2 sterile nutrient agar Petri dishes • 1 sterile blood agar Petri dish • Incubator • Refrigerator • Bunsen burner • Gas connection • Plastic tubing • Inoculating loop • 12 sterile glass slides • Wax pencil • Igniter • Crystal violet dye
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substances and absorb toxic metabolites this organism is often grown on media with rich concentrations of blood‚ or charcoal. ("Pertussis."). Two different solid cultures that are used in growing B. pertussis are Bordet-Gengou agar (BGA) and Regan-Lowe agar (RLA). Bordet- Gengou agar contains potato starch and is peptone free‚ also an inhibitory substance. It also contains glycerol as a stabilizer‚ and an antibiotic‚ such as penicillin‚ to restrain gram positive organisms from growing‚ although the
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2 were Pseudomonas aeruginosa. One of each was used to confirm. Finally only MTCC cultures were used to prepare inoculum. Inoculums Preparation Primary isolation was initiated by nutrient agar plating and subsequently transferred to selective media like Pseudomonas Isolation Agar‚ MacConkey Agar and EMB agar for better isolation. Using sterile inoculation loop colonies of the test organism are transferred to 5ml of sterile nutrient broth and incubated at 37 0C overnight for 24hrs. Then this bacterial
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This was done using an inoculating needle and aseptically transferring the bacteria into a slant of Simmon’s citrate agar by stabbing the needle into the butt of the agar‚ then streaking it across the top of the agar as the needle was pulled out. The tube was then placed in the 37 degrees Celsius incubator for 48 hours‚ observed for a blue color‚ then placed back in the incubator for another 5 days and observed again
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Buffers‚ and pH‚ and Diffusion oh my The pH of a solution is the measure of the concentration of charged Hydrogen ions in that given solution. A solution with a pH lower than seven is considered to be acidic. A solution with a higher pH is a base. It is very important for organisms to maintain a stable pH. Biological molecules such as proteins function only at a certain pH level and any changes in pH can result in them not functioning properly. To maintain these constant pH levels‚ buffer solutions
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Isolation and Identification of Staphylococcus aureus and epidermidis Wendy Heck Bio 175: General Microbiology Fall 2012-11-21 Staphylococcus aureus is the most pathogenic for humans and Staphylococcus epidermidis is part of the normal flora and is of low pathogenicity. Staphylococcus epidermidis and Staphylococcus aureus are two medically important species of bacteria. A culture from the nose and throat was taken to perform whether or not Staphylococcus epidermidis or Staphylococcus aureus
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ascus. The spores germinate and form hyphae (Campbell‚ 64) Species of the genus Sordaria share a number of characteristics that are advantageous for genetic studies. They all have a short life cycle‚ usually 7-12 days‚ and are easy to grow on nutrient agar in dish cultures. All kinds of mutants are easily induced and readily obtainable with particular ascospore color mutants. These visual mutants aid in tetrad analysis‚ especially in analysis of intragenic recombination. ( Campbell 72) A common
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responses in sensitised people‚ causing respiratory diseases such as hayfever and asthma (Knox‚ Ladiges‚ Evans and Saints 2006). A variety of techniques can be used to identify the diversity and density of fungi within the Sydney Basin. The use of agar plate exposure allows microbial colonies to culture within a sterile environment. These colonies can be identified using dichotomous keys (School of Biological Sciences 2010) under compound and dissecting microscopes. This type of analysis is appropriate
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folded hands to all my ministers and workers‚ please do not become proud. 9-We had to form a party to wade out arrogant politicians‚ hope that the no one else has to form a party to wade us out. 10-"Mera man kehta hai ki ane wale panch salon mein yeh desh sone ki chidiya ban jayenga". Arvind Kejriwal sworn in as Delhi’s seventh Chief Highlights of Arvind Kejriwal’s speech after oath taking ceremony 1-The struggle was to give power to the people of Delhi. 2-If anyone asks for bribe
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others. Two examples of selective medium are Mannitol Salt agar and Phenylethyl Alcohol. 2. What is a differential medium? What makes the medium differential? Name 2 examples (3 pts.) Differential medium is distinguishing microorganisms from one another based on growth characteristics. A medium is differential when you are able to visibly see the differences in growth patterns of organisms. Differential media include blood agar and Eosin Methylene Blue. Steps Used in Identifying an Unknown
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