aureus were maintained on Tryptic Soy agar (TSA)‚ following incubation at 37 oC for 24 h. Inocula were obtained from overnight fresh cultures adjusted to approximately log 108 CFU/ml‚ a turbidity equivalent to a 0.5 McFarland standard (Kroning et al.‚ 2016). The inocula were spread on the surface of Mueller-Hinton agar (MHA) plates. The disk diffusion test‚ recommended by the Clinical and Laboratory Standards Institute (CLSI‚
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WUS101/2 TERAS KEUSAHAWANAN SEM II 2012/2013 LAPORAN PROFIL USAHAWAN Prepared by: NORHAMIZAH BINTI IDROS 108965 930623-10-7864 KEJURUTERAAN ELEKTRONIK (EE) Prepared for: MOHD KAMIL ARIFFIN LECTURER WUS101 USM 25 APRIL 2013 Date: 20 APRIL 2013 Norhamizah Idros Kampus Kejuruteraan Universiti Sains Malaysia 14300 Nibong Tebal‚ Pulau Pinang. Mohd Shafie Ariffin Lecturer of WUS101 Universiti Sains Malaysia. Submission of WUS101
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What Sunscreen really works? Problem: Will sunscreen with an SPF of 4 be able to withstand the sunlight more effectively than SPF 45? Purpose: Due to the increasing amount of sun exposure on teens‚ we want to ensure that sunscreen can protect against the sun. An increase amount of exposure can damage the skin directly. The dangers of increased sun can lead to the 3 types of skin cancer (Basal‚ Melanoma‚ and Squamous) and these types can spread to other parts of the body. The most dangerous form
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The Treaty of Versailles The Treaty of Versailles was a controversial movement. As described in Hitlers words it is “the great villainy of the century.” (Mein Kampf). In Hitlers eyes the Treaty humiliated and further destroyed the economy in Germany. Germany had to admit complete responsibly for starting the war‚ also known as Clause 231‚ “War Guilt Clause.” He did not believe that Germany was at fault for the war‚ but instead blamed it on other groups such as the Jews and Communists. He wanted to
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Rural Urban Migration Aaapka Naam kya hai? Mera Naam “Bechaara” Hai Bechara ji..aap kahan jaa rahe ho? Mein apna gaon chod ke “Seher” Jaa raha Hoon Aap apna gaon chod ke seher kyun jaa rahe ho? Sahab jii…mein he kya…mere bahaut yaar dost jaa chuke hain..or ab mein bhi jaa raha hoon Lekin Kaaran toh bataoo….Bechara Ji Sahab jii…ek kaaran ho toh bataun…lekin aap zor de rahe ho toh sunoo.. Reasons for Rural-Urban Migration • Poverty/Low Income • • • • Unemployment Wage
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Blood agar plate This test is used to detect the hemolytic activity in the bacteria. A darkish green color on the media around the bacteria would represent incomplete hemolysis. A transparent media around the bacteria colony represents complete lysis of the red blood cells. If no change is observed around the bacteria colony then the bacteria is non-hemolytic. For my bacteria no change is observed in the media therefore the bacteria is non-hemolytic and negative result. MacConkey Agar test
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of bacteria in a given cultured sample is too great to be counted directly. The samples will be serially diluted by a factor of 10 in sterile saline‚ to be repeated 7 times. A 10 microliter aliquot from each dilution will be placed on a 5% blood agar plate or a MacConkey plate‚ depending on the type of organisms plated. These plates are selected because of the ease of differentiation between organisms. The original vial and all diluted aliquots will then be refrigerated to restrict growth until
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technique). Each group will inoculate his/her own plate of Mueller-Hinton agar with an assigned culture. To that inoculated plate‚ you will then aseptically add sterile filter paper discs (using a disc dispenser)‚ which contain a known concentration of antibiotics. As soon as the antibiotic discs touch the agar‚ the antibiotic will begin to diffuse into the surrounding agar. During incubation the bacteria you inoculated onto the agar
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This is a quantitative test and is considered to be the gold-standard method for biofilm detection (13). Organisms isolated from fresh agar plates were inoculated in 10 mL of trypticase soy broth with 1% glucose. Broths were incubated at 370C for 24 hours. The cultures were then diluted 1:100 with fresh medium. Individual wells of sterile 96 well-flat bottom polystyrene tissue culture
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Pharmaceutical Microbiology CLASS CODE: MP202 SARA ASHOUR Signature ……………………………….. Date ……………………………….. Total Word Count 1492 I declare that‚ except where specifically indicated‚ all the work presented in this report is my own and I am the sole author of all parts. Toxic shock syndrome (TSS) is an uncommon but serious systemic infection which can occur in males and females of all ages. Symptoms include fever‚ hypotension
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