glass tube test and the agar-water gel test. In the glass tube test‚ two cotton balls were soaked in two different substances‚ one in hydrochloric acid (HCl) and the other one in ammonium hydroxide (NH4OH). The cotton balls were inserted into the two ends of the tube at the same time. After some time‚ a white smoke was observed to evolve nearer the cotton ball soaked in HCl. This indicated that NH4OH‚ which is lighter‚ diffused faster than HCl. The next test was the agar-water gel test. It was composed
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Cyra Vose Klebsiella Pneumoniae #34 MCB 2010C Friday 9:00am Introduction: On November 16‚ 2012 at 0900 I was given case #34. Mr. Joe Blough presented with a sudden high fever (103 degrees Fahrenheit or higher) and mentioned he felt dizzy and had the chills. During this visit Mr. Blough seemed to be coughing and producing sputum. This sputum was thick and had some blood present. I had my assistant collect a sample of the sputum so we could run some tests and hopefully correctly diagnose Mr. Blough
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Effect of Molecular Weight on the Rate of Diffusion of Potassium permanganate (KMNO4)‚ Potassium dichromate (K2Cr2O7) and Methylene blue ¹ ABSTRACT The effect of molecular weight on the rate of diffusion was determined using the Agar-water Gel Test. One drop of potassium permanganate (KMNO4)‚ potassium dichromate (K2Cr2O7) and methylene blue were used. Each substance has a respective molecular weight of 158 g/mol‚ 294g/mol and 374 g/mol. The diameters (in millimeter) were measured
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this study. All procedures‚ unless otherwise noted‚ have been followed and performed exactly as stated in the laboratory manual Leboffe & Pierce(1). Using the Quadrant Streak technique the unknown and ubiquity were streaked onto a trypticase soy agar (TSA) plate. This method was performed as stated in the Leboffe & Pierce lab manual(1). The TSA plates were incubated at 30 degrees Celsius for 48 hours. This process was repeated until isolated colonies appeared. At this point cell morphology was
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All strains were provided by Ondokuz Mayıs University‚ the Faculty of Veterinary Medicine and Faculty of Medicine. Agar diffusion test The microbiological assays were performed under aseptic conditions inside a class 2 cabinet (Danlaf VFRS 1806‚ Denmark) previously sterilized. All testing procedures were carried out by a single operator using sterile instruments. Agar diffusion test (ADT) was used for determination of antimicrobial activities of test materials. The microorganisms inoculated
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negative control‚ 15 seconds‚ 30 seconds‚ 45 seconds‚ and 60 seconds). Aseptic technique was critical during each transfer of agars. Plain 50 agar was poured equally to each plate labeled. A cotton end of the applicator stick was wet with the liquid culture of bacteria‚ Serratia marcescens‚ and applied on the surface of agar the same way it was carried out to cover the agar gradient in the first part of the experiment. Plates were prepared for the UV exposure. It was in this step that each plate
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his experiment was done to find out if garlic is effective in killing bacteria. This will help us understand the effectiveness of home remedies such as the use of natural herbs (including garlic) for medicinal purposes. Hypothesis Garlic will kill bacteria and prevent its further growth. Background Herbs and spices have traditionally been used in cooking as they add flavor to our food. These herbs and spices have also been known to many cultures for their medicinal properties. The oils that are
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Clare Worley 6S 4 - 16 - 18 What Surface in OLV has the most Germs? Introduction I have tested several surfaces in Our Lady of Victory to see which surface has the most germs. I tested ten surfaces and let the petri dishes grow for five days. Over the five days I counted the spores each day and took pictures every other day. It is amazing how things can look and feel clean but they are actually filthy. It is also crazy how many surfaces we touch every day and how one
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Quotation 1: Yes‚ the Führer decided that he would rule the world with words. ‘I will never fire a gun‚’ he devised. ‘I will not have to.’ … His first plan of attack was to plant the words in as many areas of his homeland as possible. He watched them grow‚ until eventually‚ great forests of words had risen throughout Germany … It was a nation of farmed thoughts.” (Zusak 451) Speaker: The speaker of the quotation is Max Vandenburg. Context: In Germany‚ where Hitler rules‚ the Huberman’s have adopted
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For the completion of this experiment the procedures were guided with the Rainbow Transformation1 lab manual. An Escherichia coli bacterial reference plate was used to obtain colonies which were resuspended into a CaCl2 solution that was previously kept on an ice bath. The rainbow transformation mixture containing the plasmid DNA was then added to half of the E. coli cells. These cells were later placed into a water bath set to 42ºC and “heat shocked” to promote the entrance of DNA into the cells
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