Enzyme Catalysis Maltose sugar is broken apart by maltase enzyme Substrate are molecules enclosed in the enzyme Catalase: found in every living thing Takes two molecules of hydrogen peroxide and converts it irreversibly to create oxygen gas and water 2H2O2O2+2H2O Question: What variable affects the rate of enzyme catalysis most? Variables Tested: Hydrogen Peroxide concentration‚ yeast concentration‚ heat and pH Materials: 10% glucose mixture 1.5 %‚ 3% and 6% peroxide mixture Yeast
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The Virtual Lab – ELISA Test Lab: Immunology 09/04/2013 Instructors: Dr. Charlie Wilson Written by: Dipen Patel I. Objective: The purpose of the lab was to learn the procedure of performing an ELISA test to determine whether a particular antibody is present in a patient’s blood sample. ELISA is an abbreviation for “Enzyme-linked Immunosorbent Assay." II. Introduction: The interaction of antigen and antibody outside the body can be used to determine if patient
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Enzyme Activity How does temperature affect enzyme activity? In this practical investigation‚ my aim is to discover how temperature will affect enzyme activity‚ by looking at the rate of reaction. I predict that the higher the temperature will be‚ the faster the reactions take place. However‚ I also think that there will be an optimum temperature‚ at which the reaction will work at its fastest; if the temperature goes beyond that‚ the reactions will stop altogether as the enzymes would have
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The Effect of pH on the Rate of Enzyme Catalysis of Catalase Objectives: The objective of this lab was to develop a protocol to investigate the effect of an environmental variable on the catalytic function of an enzyme. More specifically‚ the objective was to perform an experiment in order to test the effect of pH on the function of the enzyme catalase. Introduction: Enzymes are proteins that act as catalysts for reactions. This simply means that enzymes lower the activation energy required
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Abstract The objective of this lab was to measure the amount of protein from a piece of beef liver . This was done by taking the liver‚ blending it and then using a centrifuge to separate the supernatant from the pellet. Once that was completed‚ ammonium sulfate was added to the supernatant‚ chilled and then spun for a second time. Next‚ 20 mL of water is added to the pellet‚ stirred and the volume was recorded. The teacher calculated the total mass of liver to be 10.098g. Lastly a spectronic
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Observations made that constituted a positive (+) for inotropic effect were signs of increased force in regards to the heart muscle contracting. Likewise‚ a negative (-) inotropic effect was noted for signs of less forceful contractions of the pig heart. A positive chronotropic effect was denoted by an increase in heart rate‚ and conversely a negative chronotropic effect was observed by a decrease in overall heart rate shortly after the drugs were administered. Discussion: The results obtained
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Enzyme Lab Name ___________________________ Assignment 1: Getting to Know Enzyme Lab: Setting Up an Experiment The first screen that appears in Enzyme Lab shows you a biochemistry lab containing all the reagents and equipment you will need to perform your experiments. Click on each item in the lab to learn more about its purpose. Once you are familiar with the lab‚ click on the Experiment button to begin the first assignment. This assignment is designed to help you become
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Figure 3 Superoxide radical scavenging activity. Values are mean ± SEM (n = 3). ABL‚ lyophilized aqueous extract of A. bilimbi fruits; ABP‚ petroleum ether fraction of ABL; ABE‚ ethyl acetate fraction of ABL; ABB‚ butanol fraction of ABL; ABW‚ aqueous fraction of ABL; QU‚ quercetin. 3.3.5 Nitric oxide radical scavenging activity Lyophilized aqueous extract of Averrhoa bilimbi fruits (ABL) and its different solvent fractions exhibited a dose dependent inhibition of nitric oxide radicals. The IC50
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The Effect of Enzymes on Apple Juice Production Biology Period 6 Background Info The purpose of this experiment was to determine which enzyme or the combination of the two enzymes makes the most apple juice from applesauce. We did his by‚ mixing 10 drops of an enzyme (cellulose/pectinase/both) into a few tablespoons of applesauce. We then left the rest to nature‚ and watched the liquid funnel out for 10 minutes. Lastly‚ we recorded our data. The cell wall is a complicated
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The hypothesis for this experiment was that if the baby lemurs activity level was compared to an adults‚ the baby lemur will have a higher activity level. The hypothesis was not supported. The data shows that the adult had a higher activity level. The data shows the adult being 1.8% more active than the baby lemur. The other behaviors of the adult Ring Tailed Lemur was grooming and sleeping/resting. Second‚ throughout the six trials the only behaviors that were performed by the baby lemur was sleeping/resting
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