seen at the top of the solution. Lead Acetate Cloudy solution with small specks of white precipitate. Ice cold ethanol Cloudy at the bottom and about 0.5 cm of the top of the solution was clear. Table 4: The results of Gel Filtration Chromatography Eluate Component Volume collected in mL First Blue dextrin 3.00 Second Brown cytochrome 7.00 Third Clear phosphate buffer 5.00 Table 5: The result of Electrophoresis of Amino Acids Distance
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ORGANIC CHEMISTRY 1 LAB EXPERIMENT NO. 1 to 6 DISCUSSION EXPERIMENT NO. 1: Mel=ng Points And Boiling Points Of Organic Compounds Mel=ng Point -‐ temperature at which the liquid and solid are in equilibrium at a pressure of 1 atm Mel=ng Point Range -‐ determines the purity of a solid sample -‐ temp at
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High Performance Liquid Chromatography Analysis of Aspirin Problem: Was aspirin (acetylsalicylic acid) successfully synthesized? Are there impurities or by-products present in the synthesized aspirin? How pure is the synthesized aspirin? Introduction: In the last experiment‚ aspirin was synthesized followed by characterization of the product using several different techniques. Melting point was a test that provided information about the identity and purity of the aspirin product. The iron(III)chloride
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crystals started to melt in the fast ramp. The end temperature was 5°C above the end temperature in the fast ramp‚ and the ramp rate was 1°C /min. After running the slow ramp the melting point range was compared to possible known compound. The compound Fluorene was a possible match for what the unknown compound was. To confirm that the unknown compound was Flourene a mixed melting point was ran. In a weighing paper the unknown compound and flouerene was mixed and 1-2mm was placed in a capillary tube. A
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room temperature and gently poured over the resin. The tube was capped and kept on a rocker at room temperature for 1 hour. The tube was then centrifuged in a HS-4 rotor at 2500 rpm (1200g) for 5 minutes at 4ᵒ C. Supernatant was discarded and the column was washed twice (i.e. centrifuged); first in 30 mL of low salt Buffer C (20 mM HEPES‚ pH 7.9; 1 mM EDTA; 50 mM KCl)‚ followed by 30 mL of high salt Buffer C (20 mM HEPES‚ pH 7.9; 1 mM EDTA; 0.2 M KCl). Each time after adding the buffers‚ PMSF (protease
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MCOPS‚ Manipal ANALYTICAL METHODS FOR RESIDUAL SOLVENTS INTRODUCTION: Organic solvents are routinely applied during synthesis of drug substances‚ excipients‚ or during drug product formulation. They are not desirable in the final product‚ mainly because of their toxicity‚ their influence on the quality of crystals of the drug substance and their odor or taste‚ which can be unpleasant for patients. To remove them‚ various manufacturing processes or techniques (usually under
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such as activity and protein assay were employed to determine the presence and purity of LDH. The cells were initially disrupted and proteins were solubilized. LDH was purified from the ammonium sulfate precipitated protein mixture by affinity chromatography and its activity was studied by spectrophotometric determination of NADH at 340 nm. From Pierce BCA assay of crude homogenate‚ initial protein concentration was shown to be 100 mg/ml. The final protein concentration of the pooled affinity sample
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Experiment II. Separation Of a Sample Mixture By Liquid-Liquid Extraction Reading assignment: Techniques in Organic Chemistry 2nd ed pages 75-99. 3rd ed pages 113-140. Topics and Techniques i) identification of solvent layers of two immiscible solvents ii) partioning of a compound between two immiscible solvents and determination of KD iii) liquid-liquid extraction with aqueous acids and bases with organic solvents. iv) use of drying agents Introduction Liquid-liquid extraction is a method
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inhibiting enzymatic reactions involving para-aminobenzoic acid (PABA). PABA is needed in enzymatic reactions that produce folic acid which acts as a coenzyme in the synthesis of purine‚ pyrimidine and other amino acids. In part C‚ Fluorene was dissolved in three solvents. Fluorene(C13H10) is a polycyclic aromatic hydrocarbon. It forms white crystals that exhibit a characteristic‚ aromatic odor similar to that of naphthalene. It is combustible. It has a violet fluorescence‚ hence its name. For commercial
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binds the nucleoside inhibitor site. When it binds this site‚ it stabilizes the inactive T state and blocks the catalytic site which needs to be open for enzyme activity to occur. The glycogen phosphorylase b was purified with hydrophobic column chromatography and the concentration was determined with a Bradford Assay. The kinetics of glycogen phosphorylase b were studied by finding a molar extinction coefficient‚ 0.1617 mM cm-1 A-1‚ for
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