The concentration of 1-kestose isomer increased steadily with time from 0 - 80 min. During this time‚ sucrose was significantly influencing the rate of 1-kestose formation and hence the optimum concentration of the 1-kestose isomer was observed at 80 min. After 80 min‚ the 1-kestose concentration gradually decreased. This was because the invertase active sites had become saturated with sucrose (substrate) or sucrose had been used up in the reaction. Therefore‚ there was a gradual decrease observed
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this lab‚ our goal was to look at the effects of different sucrose concentrations on osmosis in potatoes. Our research question was “How do the sugar levels affect the rate of osmosis in potatoes?”. Background Information: Solutions consist of solutes dissolved in a solvent. In all living organisms there are many different types of solutes including salts and sugars. The major solvent is water. There are different concentrations of solutes in various regions of an organism‚ including differences
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Why should the solution with highest concentration be used to determine the analytical wavelength? 3. What was the analytical wavelength of FeSCN2+? 4. Relate the analytical wavelength to the color of the solution. Hint: Use a color wheel. 5. Why should we measure absorbance at the analytical wavelength? Calibration 6. What is the importance of the blank solution? 7. Why is KSCN added in large excess? 8. What is the concentration of FeSCN2+ for each solution? 9. Determine
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Investigate osmosis between potato tissue and sugar solutions. Aim: To investigate the effect of varying concentration of a certain sugar solution on the amount of osmotic activity between the solution and a potato core of a given size 10mm Hypothesis: Osmosis is defined as the net movement of water or any other solutions molecules from a region in which they are highly concentrated to a region in which they are less concentrated. This movement must take place across a partially permeable
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using diffusion and osmosis you are trying to separate different solute concentrations on either side of the membrane. Only a solute’s relative concentration‚ or water potential‚ affects the rate of osmosis. The higher the concentration of solutes‚ the faster water will flow through the membrane to equalize the concentration. The way we describe the movement from higher to lower concentration or lower to higher concentration is called hypotonic solution and hypertonic solution. Hypothesis:
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depend on two things. 1. The length of the pathway of light 2.Concentration of colour solution. These are all explained by the Beer-Lambert Law. Absorbance (A) = ɛcl A= Absorbance ɛ = molar absorption coefficient (Depend on unit concentration ) c = concentration of colour solution. l = length of the light path. In here two experiments were carried out to clarify the relationship between concentration and absorbance in different wavelengths. According to the Beer-Lambert
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curve‚ determine the concentrations for control and patient specimens. Materials and Reagents: 2ml 1M Sulfuric Acid 2ml of 0.1M Potassium Dichromate Distilled Water Pipettes Volumetric Flask Test Tubes Procedure: 1. Prepare 1 ml glucose solution and place in test tubes 2. Add 2ml of 1M Sulfuric Acid solution 3. Add 2ml of 0.1M Potassium Dichromate solution 4. Start timing until the color disappear Results and Discussion: The fastest concentration of glucose to react
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shrink. 3. The variable that affects osmotic pressure is You correctly answered: a. the concentration of nondiffusing solutes. 4. The net movement of water would be into the cell in a Your answer : d. hypnotonic solution. Correct answer: b. hypotonic solution. 01/20/14 page 1 Experiment Results Predict Question: Predict Question 1: What effect do you think increasing the Na+ Cl- concentration will have? Your answer : a. increased osmotic pressure Predict Question 2: What do you
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the random movement of its molecules results in a multitude of collisions. These collisions‚ in turn‚ lead to a dispersion of the molecules. The overall movement of the molecules will be from an area of high concentration‚ where there will be more collisions‚ to areas of low concentration‚ where the number of collisions will be much less. This process of dispersion will continue until there is no net gain or loss of molecules in an area. The process by which this equilibrium occurs is called diffusion
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The majority of the experimentation in this project relied on the idea that there is a relationship between concentration and absorbance of a solution. This is easy to understand if you can visualize the particles in the solution; and this is especially true if the particles in solution portray a distinct color. The more particles there are in the solution‚ the more they will obstruct the path of the light. This occurs the most at the analytical wavelength‚ where the most light is absorbed by
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