cells use catalase to destroy cellular debris or worn out organelles. In this lab‚ we will use a catalase solution from potatoes and determine the effect of temperature and pH on the action of this enzyme. The substrate of the enzyme will be 3% hydrogen peroxide (H2O2). Catalase works by the following mechanism: 2 H2O2 ------------------>____2 H2O+ O2 Materials: • 1M HCl • 6 test tubes • hot water bath • graduated cylinder • 1M • • NaOH • test tube rack
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used is different concentrations of potato and the reactant used will be Hydrogen Peroxide. Hydrogen Peroxide which will be the buffer solution is a PH of 7.2. My hypothesis for the experiment is that as the concentration of the enzyme is increased the rate of reaction will be increased‚ producing oxygen at a faster rate. The results in my opinion will show positive correlation. The amount of buffer solution (hydrogen peroxide) will be kept the same throughout the experiment in the interest of reliability
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Enzyme Catalysis Abstract: Catalysis is an enzyme that decomposes hydrogen peroxide into oxygen and water. In this lab we will conduct a series of experiments to determine the affects of; pH‚ temperature‚ and concentration change on such catalysis. After completing the experiments we determined that catalase works most efficiently when in a 27°c‚ 50% (5 ml of catalase – 5ml of water)‚ and a normal body pH of 7°. By: Patrick Jawien Course Code: SBI 4U Performance Date: 25 September 2012
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factors help enzymes break down the poisonous chemicals into harmless substance. When we tested the liver with 2ml of hydrogen peroxide for a normal reaction it showed that it was exothermic. We added more hydrogen peroxide and the reaction rate of the liver was 3. We learned that the catalase is reusable because the liver reacted both times when we put in the hydrogen peroxide. We also tested another enzyme reaction for pH. In this test we learned that high pH equal high reaction rate. The 4 items
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without having to change its own shape‚ which makes enzymes different from other proteins. A common enzyme that we have is catalase‚ which breaks down hydrogen peroxide into the products water and oxygen. To observe this catalase activity I used small amounts of liver samples slightly smaller than the size of a pea to see the reaction when hydrogen peroxide is added to it. In this experiment I exposed the liver samples in three different temperature environments: 3°c‚ 22°c‚ and 75°c. I wanted to measure
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Prepared by Anel Behric For professor Goodrow General Biology Lab Mohawk Valley Community College 10/14/2014 6:00 PM Introduction: This experiment was conducted to examine the breakdown of substrate hydrogen peroxide (H2O2) by catalase‚ which is a specific enzyme that breaks down substrates of (H2O2). Molecules are in our bodies and nature. They move around constantly which causes them to be part of random collisions‚ which produce chemical reactions. This
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A Team Project Entitled: Feasibility of Mustard (Brassica Integrifolia) Root Extract for Water Phenol Decontamination ANNA KRIZA D. LACHICA MA. ANGELICA V. RAMOS RESEARCHERS MS. CRISTINE SEALZA SAÑADA RESEARCH TEACHER February 7‚ 2011 Table of Contents Acknowledgement.....................................................................................................3 Abstract ...............................................................................................
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temperature range and limited sample size at each group. The experiment also existed two other uncontrolled variables which are the size of liver and the amount of Hydrogen Peroxide‚ hence altering the authenticity of the practical result. At each of the five experimental group‚ there were variations amongst the amount of Hydrogen Peroxide and the size of beef liver‚ which can be a problem because in a well controlled and designed experiment‚ there is only one independent variable. These uncontrolled
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what affects the rate of reaction. We used Hydrogen peroxide to test the rate of reaction‚ with the temperature of this being our variable that we changed. Hydrogen peroxide is a clear‚ colourless liquid which has various amounts of uses within the laboratory‚ industrial purposes and even in our households. It is mainly used for cleaning products and hair dye but is also used for paper making. Hydrogen peroxide is made up of two oxygen atoms and two hydrogen atoms. The chemical formula for this is H₂O₂
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The 0% concentration of Hydrogen Peroxide was used as our control. The 3% concentration of Hydrogen Peroxide was provided so all that was needed to do was prepare the 0%‚ 1%‚ and 2% concentrations which was done by using the equation C₁V₁ = C₂V₂. Other materials consisted of pH 7 buffer‚ guaiacol which was used as our
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