Biology investigation: Liver and hydrogen peroxide By Cuong Tran Abstract: The purpose of this investigation was to determine the optimum temperature for enzyme activity. The stated hypothesis was that 37oC would be the optimum temperature for liver enzymes to break down hydrogen peroxide into water and oxygen. The dependent variable was to measure the amount of foam being produced‚ while testing 4 different temperatures with 3 repetitions each. Out of the 12 trials only 2 outliers were found
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Available online 8 December 2004 Abstract The encapsulation of enzymes in microenvironments and especially in liposomes‚ has proven to greatly improve enzyme stabilization against unfolding‚ denaturation and dilution effects. Combining this stabilization effect‚ with the fact that liposomes are optically translucent‚ we have designed nano-sized spherical biosensors. In this work liposome-based biosensors are prepared by encapsulating the enzyme acetylcholinesterase (AChE) in L-a phosphatidylcholine liposomes
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“Investigate the factors affecting the rate of yeast respiration” Lab Report Introduction The aim of this experiment was to investigate the effect of different amounts of a substrate on the respiration rate of yeast and to compare this to the effect of different amounts of glucose on the rate of yeast respiration. The substrate which I chose to further investigate was fructose. Fructose is a fruit sugar which is one of the three‚ along with glucose and galactose‚ dietary monosaccharides that
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Studying the Rate of Reaction of catechol oxidase and how it affects pH levels Introduction: In this lab‚ we studied the activity of an enzyme that is found in fruits and vegetables called catecholase or catechol oxidase. An enzyme is a protein molecule that is a catalyst. Catechol oxidase is the enzyme in fruits and vegetables that turns them that undesirable brownish color; also commonly referred to as bruising or bruised. When walking through your regular grocery store and you find yourself in
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the enzyme? Denaturing the enzyme is important because leaving it active will increase the activity of the enzyme. 1. State the optimum pH for sucrase activity and how sucrase activity changes at more acidic and alkaline pH values. 2. State the optimum temperature for sucrase activity and how sucrase activity changes at lower and higher temperatures. 3. State how sucrase activity changes with increasing sucrose concentration. Discussion 1. Explain how pH and temperature affect enzyme activity
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week 3 problem set. Enzymes and energy 1.Many spontaneous reactions occur slowly. Why don’t they all occur instantly? In biological processes‚ how is this rate sped up? 2. When the substrate (reactant) concentration is high enough enzyme A and enzyme A’ may be saturated and their reactions run at the same maximum velocity. However‚ at subsaturating concentrations of reactants‚ these different enzymes run at different velocities for the same concentration of substrate. Enzyme A’ has a Km that is
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to determine if there is a minimum temperature that increase kinetic energy and denature enzymes to slow enzymatic reactions or fail to catalyze them. The experimental results indicate an increase in temperature will increase reaction rates until proteins denature. Introduction Enzymes combine with reactant molecules (substrate) and bind them closely to one another. The three-dimensional shape of the enzyme molecule must be complementary to the shape of the substrate. Catalysts are not used up
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Def: An enzyme is a protein formed by the body that acts as a catalyst to cause a certain desired reaction. Enzymes are very specific. Each enzyme is designed to initiate a specific response with a specific result. There are many enzymes in the human body. In cystic fibrosis‚ the term enzyme generally refers to the enzymes created by the pancreas that are intended to initate a reaction that digests food. Structure :The basic mechanism by which enzymes catalyze chemical reactions begins with
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What affect does boiling have on enzyme activity? a. Boiling inactivates‚ or denatures‚ enzymes. Activity 2 Which tubes showed that starch or cellulose was present? a. 4‚ 5 and 6 Which tubes tested positive for the presence of reducing sugars? a. 1‚ 2‚3 and 7 What was the affect of freezing tube 1? a. Freezing had no affect How does the effect of freezing differ from the affect of boiling? a. Freezing does not restrict enzyme activity Does amylase use
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Glucose 6-phosphate is transferred to NADP which soon becomes NADPH. NADPH is accumulated and later used for biosynthesis. *Fate of Ribulose 5-phosphate Ribulose 5-phosphate can now proceed to different enzymatic reactions. It is acted by an enzyme (Not specified) and converting it into ribose 5-phosphate. Oxidizing it and making it into ribose 5-phosphate. Ribulose 5-phosphate and xylulose 5-phosphate can later be converted into carbohydrate intermediates (fructose 6-phosphate) which are present
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