Abstract The enzyme peroxidase has been shown to break down H2O2. Enzymes are known to increase the rate at which a chemical reaction occurs. We looked at factors that affected the breakdown of hydrogen peroxide. These effects are the different temperatures and pH levels the enzymes were placed in. We found that the optimum‚ or best condition‚ temperature for the enzymes tested was about 22 degrees Celsius. The optimum pH level for the enzyme was 7. Introduction Enzymes are biochemical that catalyze
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hydrogen ion concentration of a substrate. By changing the pH of the catalase‚ the enzyme was denatured. Denaturing is the result of altering the characteristics or properties of an enzyme by temperature‚ pH level‚ or substrate concentrate. As the optimal conditions of the enzyme are changed‚ bonds in the enzyme are broken and the enzyme unfolds at the tertiary and quaternary level. When this happens‚ the structure of the enzyme and active site is changed. With the change in the active site‚ the substrate
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BIOLOGY 22 MODULE 1 – Chemical Basis of Life v2.0 * Levels of Organization – biological functions are ultimately based on the properties of atoms and molecules * Subatomic particles – neutrons‚ electrons‚ protons * Atoms * Compounds * Complexes of compounds * Organelles – bodies within cells that perform specific functions * Cell * Specific combination of organelles * Can metabolize and reproduce * Least elaborate living structure * Significance
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purpose of the first week experiment was to determine the effect of Nitrophenyl phosphate concentration‚ the substrate‚ on the enzyme‚ acid phosphatase‚ reaction. In an enzyme reaction‚ there is substrate that binds to the active site of the enzyme and product is the end result. The enzyme is the catalyst for the reaction which means that it speeds up the process. Without enzymes‚ the majority of reactions would not occur because the activation energy level would be unattainable. With this in mind‚ the
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between other cuvettes‚ for example‚ absorbance differences between cuvette 1s and 2s or 2s and 3s. It is assumed that the relative concentration of enzymes does not catch up that of iron cofactors. In other words‚ even though we put more iron cofactors to interact with enzymes after a certain point‚ it cannot speed the reaction further because no more enzymes can interact with extra iron cofactors. Furthermore‚ we can notice that even though the higher amount of iron cofactors indicates the higher absorbance
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How does temperature affect the rate of reaction for Lipase? As the temperature increases‚ so will the rate of enzyme reaction. However‚ as the temperature exceeds the optimum the rate of reaction will decrease. I predict that at temperatures above 70°C the enzyme lipase will become denatured and at temperatures below 10°C the enzyme will become inactive. Since lipase operates within the human body I’d also predict that its optimum temperature would be around human body temperature which is approximately
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Science Coursework Trypsin Investigation Aim I am going to prove that temperature effects the rate in which the enzyme Trypsin breaks down milk substrate into amino acids. Investigation "We strongly recommend the use of Vanish Stain Removal Tablets in conjunction with a 900 spin wash at 70°C" This was found on a milk carton of a well-known drinks company‚ Nesquick. The major food group constituent of milk is‚ of course‚ milk. For my Science Investigation‚ I am going to test different temperatures
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determine the effect of various factors on the rate of reaction between an enzyme and its substrate‚ and also to determine the optimal ranges under which the enzyme activity is maximized. Also to determine whether saline and alcohol are inhibitors or activators Hypothesis: PH factor prediction: I predict that as the pH increases so the activity of the enzyme will increase until it reaches optimum pH range (pH 7) because the enzyme is less denatured when it reaches the preferred pH level‚ and after this
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experiments are: Lactase Enzyme and Lactose. Lactase is a protein located in the human small intestine‚ while lactose is a term to define the sugar found in milk. Milk is a substance that contains nutrients necessary for our bodies‚ however‚ some people are unable to consume lactose related products‚ and so they are medically classified as Lactose Intolerants. The purpose of this experiment is to examine and illustrate the way that lactose gets affected by lactase enzyme whenever lactase is presented
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Biology investigation: Liver and hydrogen peroxide By Cuong Tran Abstract: The purpose of this investigation was to determine the optimum temperature for enzyme activity. The stated hypothesis was that 37oC would be the optimum temperature for liver enzymes to break down hydrogen peroxide into water and oxygen. The dependent variable was to measure the amount of foam being produced‚ while testing 4 different temperatures with 3 repetitions each. Out of the 12 trials only 2 outliers were found
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