“Investigate the factors affecting the rate of yeast respiration” Lab Report Introduction The aim of this experiment was to investigate the effect of different amounts of a substrate on the respiration rate of yeast and to compare this to the effect of different amounts of glucose on the rate of yeast respiration. The substrate which I chose to further investigate was fructose. Fructose is a fruit sugar which is one of the three‚ along with glucose and galactose‚ dietary monosaccharides that
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Appendix 14 1.0 Abstract In this experiment‚ Escherichia coli is used as a sample to study the growth kinetic of microorganism in shake flask. A different volume of E.coli was transferred into 250ml Erlenmeyer/shake flask containing media for the nutrient of microorganism. The different volume of microorganism transferred will give the different effect of reading on the constant volume of media used. There are three ways to test the growth kinetic rate of microorganism on shake flask‚ which are
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Purpose: To find which of solutions will produce the most alcoholic fermentation by measuring the depth of the carbon dioxide bubbles and the diameter of the balloon. Apparatus and Material: Funnel 4 test tubes Cups Sugar Water Yeast Knife Ruler Balloon Marker Tape Method: 1) Put tape on each test tube and label them‚ 0%‚ 1%‚ 5% or 10%. 2) Fill up water in each cup. 3) Add 10 ml of water in each test tube 4) For the test tube labeled 1%‚ add 0.1 ml of sugar. 5) Add 0.5 ml of sugar
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Yeast Population Lab Report During this experiment we were trying to determine how food availability affects CO2 production (related to population growth). We investigated how one factor influences the change in yeast population growth as measured by the amount of carbon dioxide produced. The yeast that you buy in the store contains living organisms–invisible small one celled‚ microorganisms. As long as they are kept dry‚ they are inactive. When they are given food‚ moisture and warmth‚ they
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this project is to study the rates of fermentation of the following fruit or vegetable juices. i. Apple juice ii. Carrot juice INTRODUCTION Fermentation is the slow decomposition of complex organic compound into simpler compounds by the action of enzymes. Enzymes are complex organic compounds‚ generally proteins. Examples of fermentation are: souring of milk or curd‚ bread making‚ wine making and brewing. The word Fermentation has been derived from Latin (Ferver which
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Effects of SO2 On Fermentation Rates Purpose SO2 is the primary inhibitor for natural microbiological growth in wine. It prevents the browning of juice by inhibiting phenol oxidase activity and kills the natural yeast cells for the utilization of fermentation-controlled commercial Saccharomyce strands (Boulton et al. 1996). SO2 is pH and temperature dependent and can exist as several forms. The bisulfate form (HSO3-) can complex with soluble solids such as anthocyanins and acetaldehydes to become
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Bio coursework Methylene blue Yeast cells explanation of respiration hence colour change etc Low temp colour change should be visible as the yeast cells are not necessarily dead‚ just inactive. Activity increases from 20-45 c High rate around 30-40 Starts to slow down basically enzyme curve see bio 1 100 degrees will kill all cells Do a few preliminary keep working down until first blue solution appears in unit of ten Then work to find degree. If more accuracy then half
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that is known as fermentation. Sugars in the form of sucrose are mostly used by humans (Alexander N.Glazer). Sugar cane and sugar beet are composed of 20% sucrose‚ 75% water‚ 5% cellulose and about 1% is inorganic salts (Alexander N.Glazer). Sucrose is extracted with water by using mechanical pressure from the sugarcane. On the other hard the beet is peeled and crushed. Sucrose that was extracted from sugarcane is a highly effective substrate that is used during yeast fermentation (Alexander N.Glazer)
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Carbohydrates Carbohydrates are the most important nutrient for the body as it is the largest possible source of energy. Carbohydrate supplies the body with energy‚ which allows the brain to function more effectively and allows organs to operate sufficiently. When the body has energy this allows each person to perform a variety of exercise regimes such as high intensity‚ fat burn and continuous training. When the body consumes carbohydrates the digestive system changes carbohydrates to glucose
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The methylene blue staining procedure is used to measure yeast viability based on the assumption that the methylene blue will enter the cells and be broken down by living yeast cells that produce the enzymes which breaks down methylene blue‚ leaving the cells colourless. The non- viable cells do not produce this enzyme (or enzymes) and as such the methylene blue that enters the cells are undegraded causing the cells to remain coloured (the oxidized form concentrates intracellularly). The coloured
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